高级搜索

阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物

,  ,  , 

downloadPDF

上一篇

下一篇

严杰能, 许燕滨, 段晓军, 杜妍晔. 阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物[J]. 环境化学, 2010, 29(2): 262-266.
引用本文: 严杰能, 许燕滨, 段晓军, 杜妍晔. 阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物[J]. 环境化学, 2010, 29(2): 262-266.
shu
YAN Jie-neng, XU Yan-bin, DUAN Xiao-jun, DU Yan-ye . EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCE FROM A Cr(Ⅵ)-REMOVAL PREDOMINATED STRAIN(Bacillus sp.)BY CATION RESIN METHOD[J]. Environmental Chemistry, 2010, 29(2): 262-266.
Citation: YAN Jie-neng, XU Yan-bin, DUAN Xiao-jun, DU Yan-ye . EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCE FROM A Cr(Ⅵ)-REMOVAL PREDOMINATED STRAIN(Bacillus sp.)BY CATION RESIN METHOD[J]. Environmental Chemistry, 2010, 29(2): 262-266.
shu

阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物

  • 1.

    广东工业大学环境科学与工程学院, 广州, 510006

  • 基金项目:

    国家自然科学基金项目(40801194)

EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCE FROM A Cr(Ⅵ)-REMOVAL PREDOMINATED STRAIN(Bacillus sp.)BY CATION RESIN METHOD

  • 1.

    Faculty of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, 510006, China

  • Fund Project:

  • 摘要: 采用阳离子交换树脂法对一株除铬优势菌(Bacillus sp.)的胞外聚合物(EPS)进行提取,综合考察树脂量、振摇频率、提取时间三个主要因素对EPS提取效率的影响,确定阳离子交换树脂法提取优势菌Bacillus sp.的最佳EPS提取条件.试验结果表明:当菌悬液OD为0.833,树脂量20g,振摇频率120r·min-1,提取时间8h时,EPS的提取效果最佳,提取量高达132.15μg·ml-1菌悬液,而DNA提取量仅1.48μg·ml-1菌悬液.
    Abstract: Cation resin method was used to extract the EPSof a Cr(Ⅵ)-removal predominated strain(Bacillus sp.),and the optimum extraction conditions,including the cation resin dose,shaking frequency and extracting time,were obtained.The experimental results showed that when the ODvalue of the bacterial suspension liquid was 0.833,with 20g cation resin,120r·min-1 and 8 h extracting time.the extraction capacity of EPSwas 132.15μg·ml-1.And the extraction capacity of DNAwas only 1.48 μg·ml-1.The cation resin method was efficient and reliable for the EPSextraction of the Cr(Ⅵ)-removal predominated strain(Bacillus sp.).
  • 加载中
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  980
  • HTML全文浏览数:  980
  • PDF下载数:  459
  • 施引文献:  0
出版历程
  • 收稿日期:  2009-07-27
严杰能, 许燕滨, 段晓军, 杜妍晔. 阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物[J]. 环境化学, 2010, 29(2): 262-266.
引用本文: 严杰能, 许燕滨, 段晓军, 杜妍晔. 阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物[J]. 环境化学, 2010, 29(2): 262-266.
shu
YAN Jie-neng, XU Yan-bin, DUAN Xiao-jun, DU Yan-ye . EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCE FROM A Cr(Ⅵ)-REMOVAL PREDOMINATED STRAIN(Bacillus sp.)BY CATION RESIN METHOD[J]. Environmental Chemistry, 2010, 29(2): 262-266.
Citation: YAN Jie-neng, XU Yan-bin, DUAN Xiao-jun, DU Yan-ye . EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCE FROM A Cr(Ⅵ)-REMOVAL PREDOMINATED STRAIN(Bacillus sp.)BY CATION RESIN METHOD[J]. Environmental Chemistry, 2010, 29(2): 262-266.
shu

阳离子交换树脂法提取一株除铬(Ⅵ)优势菌(Bacillus sp.)的胞外聚合物

  • 1. 广东工业大学环境科学与工程学院, 广州, 510006
  • 收稿日期:  2009-07-27
基金项目:

国家自然科学基金项目(40801194)

摘要: 采用阳离子交换树脂法对一株除铬优势菌(Bacillus sp.)的胞外聚合物(EPS)进行提取,综合考察树脂量、振摇频率、提取时间三个主要因素对EPS提取效率的影响,确定阳离子交换树脂法提取优势菌Bacillus sp.的最佳EPS提取条件.试验结果表明:当菌悬液OD为0.833,树脂量20g,振摇频率120r·min-1,提取时间8h时,EPS的提取效果最佳,提取量高达132.15μg·ml-1菌悬液,而DNA提取量仅1.48μg·ml-1菌悬液.

English Abstract

    全文HTML

参考文献 (0)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心