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不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能

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宋丹丹, 梁生康, 尚玉俊, 王秀莉. 不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能[J]. 环境化学, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
引用本文: 宋丹丹, 梁生康, 尚玉俊, 王秀莉. 不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能[J]. 环境化学, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
shu
SONG Dandan, LIANG Shengkang, SHANG Yujun, WANG Xiuli. Properties of sophorolipid produced by the yeast Candida bombicola grown on different lipophilic carbon substrates[J]. Environmental Chemistry, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
Citation: SONG Dandan, LIANG Shengkang, SHANG Yujun, WANG Xiuli. Properties of sophorolipid produced by the yeast Candida bombicola grown on different lipophilic carbon substrates[J]. Environmental Chemistry, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
shu

不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能

  • 1.

    河南省科学院高新技术研究中心, 郑州, 450002;

  • 2.

    中国海洋大学海洋化学理论与工程技术教育部重点实验室, 青岛, 266100

  • 基金项目:

    国家自然科学基金(41371314)资助.

Properties of sophorolipid produced by the yeast Candida bombicola grown on different lipophilic carbon substrates

  • 1.

    High & New Technology Research Center of Henan Academy of Sciences, Zhengzhou, 450002, China;

  • 2.

    Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao, 266100, China

  • Fund Project:

  • 摘要: 采用不同碳源,如油酸、菜籽油、棉籽油和煎炸废油作为假丝酵母菌(Candida. bombicola)ATCC 22214发酵生产槐糖脂的脂溶性碳源,考察了在不同碳源条件下该菌株合成槐糖脂的能力、所产槐糖脂的结构及表面活性特点.结果表明,以油酸、菜籽油及棉籽油为脂溶性碳源时,该菌株有着较高的槐糖脂产量,分别为101、73、51 g·L-1;通过高效液相色谱-高分辨质谱(HPLC-MS/MS)对以不同脂溶性碳源为发酵底物产生的槐糖脂进行分析,发现所产槐糖脂均由乙酰基取代的内酯型和酸型槐糖脂同系物构成且以内酯型槐糖脂结构为主.槐糖内酯中脂肪酸基的结构有所差异,但主要以脂肪链不饱和度为1的十八烯酸(C18:1)结构为主.所产槐糖脂具有较低的表面张力和临界胶束浓度(CMC),其中最低表面张力结果一致约36.0—37.0 mN·m-1,而CMC有所差异.同时,该槐糖脂具有良好的稳定性,其水溶液的最低表面张力在100 ℃下保持2 h基本不变,在0—20%的NaCl盐溶液及pH值为2—10范围内均保持良好的表面活性.
    Abstract: In the present study, sophorolipids were produced by yeast Candida bombicola ATCC 22214 when cultured on various lipophilic carbon substrates, such as oleic acid, rapeseed oil, cottonseed oil, and frying waste oil. The structure and surface active properties were investigated. Fed-batch fermentations of Candida bombicola resulted in sophorolipids yields of 101, 73 and 51 g·L-1 from oleic acid, rapeseed oil, and cottonseed oil. The structures of individual sophorolipid components were quantified and identified by HPLC-MS/MS system. Results showed that the products were heterogeneous mixtures of acetylated lactonized and acidic sophorolipids, and the large majority of the sophorolipids existed in the lactonic forms. Among the lactonized sophorolipids, the predominant component is sophorolipid containing hydroxyoleic acid (C18:1) as the lipid moiety. Sophorolipids had high surface activity, such as low surface tension and CMC and good stability. The minimum surface tension of the produced sophorolipids in aqueous solution were found to be 36.0—37.0 mN·m-1 regardless of the carbon substrates while disparity occurred in the CMC values. Unhindered surface activity of the sophorolipids was found at wide range of pH(2—10), temperature (heating at 100 ℃ for 2 h) and salt concentrations (0—20% NaCl).
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  • [1] Pekin G, Vardar-Sukan F, Kosaric N. Production of sophorolipids from Candida bombicola ATCC 22214 using Turkish corn oil and honey[J]. Engineering in Life Sciences, 2005, 5(4): 357-362
    [2] Hu Y, Ju L K. Sophorolipid production from different lipid precursors observed with LC-MS[J]. Enzyme and Microbial Technology, 2001, 29(10): 593-601
    [3] Van B I N A, Zhang J X, Soetaert W. Microbial synthesis of sophorolipids[J]. Process Biochemistry, 2011, 46(4): 821-833
    [4] Thavasi R, Jayalakshmi S, Balasubramanian T, et al. Biosurfactant production by Corynebacterium kutscheri from waste motor lubricant oil and peanut oil cake[J]. Letters in Applied Microbiology, 2007, 45(6): 686-691
    [5] Solaiman D K Y, Ashby R D, Zerkowski J A, et al. Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola[J]. Biotechnology Letters, 2007, 29(9): 1341-1347
    [6] 宋丹丹, 梁生康, 王江涛. 槐糖脂生物表面活性剂的结构特征及理化性质初探[J]. 环境化学, 2011, 30(8): 1474-1479
    [7] Solaiman D K Y, Ashby R D, Nunez A, et al. Production of sophorolipids by Candida bombicola grown on soy molasses as substrate[J]. Biotechnology Letters, 2004, 26(15): 1241-1245
    [8] Ratsep P, Shah V. Identification and quantification of sophorolipid analogs using ultra-fast liquid chromatography-mass spectrometry[J]. Journal of Microbiological Methods, 2009, 78(3): 354-356
    [9] Hu Y, Ju L K. Purification of lactonic sophorolipids by crystallization[J]. Journal of Biotechnology, 2001, 87(3): 263-272
    [10] Nunez A, Ashby R, Foglia T A, et al. Analysis and characterization of sophorolipids by liquid chromatography with atmospheric pressure chemical ionization[J]. Chromatographia, 2001, 53(11): 673-677
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  • 收稿日期:  2014-12-08
  • 刊出日期:  2015-07-15
宋丹丹, 梁生康, 尚玉俊, 王秀莉. 不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能[J]. 环境化学, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
引用本文: 宋丹丹, 梁生康, 尚玉俊, 王秀莉. 不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能[J]. 环境化学, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
shu
SONG Dandan, LIANG Shengkang, SHANG Yujun, WANG Xiuli. Properties of sophorolipid produced by the yeast Candida bombicola grown on different lipophilic carbon substrates[J]. Environmental Chemistry, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
Citation: SONG Dandan, LIANG Shengkang, SHANG Yujun, WANG Xiuli. Properties of sophorolipid produced by the yeast Candida bombicola grown on different lipophilic carbon substrates[J]. Environmental Chemistry, 2015, 34(7): 1252-1258. doi: 10.7524/j.issn.0254-6108.2015.07.2014120802
shu

不同碳源培养条件下假丝酵母菌产槐糖脂的结构及性能

  • 1.  河南省科学院高新技术研究中心, 郑州, 450002;
  • 2.  中国海洋大学海洋化学理论与工程技术教育部重点实验室, 青岛, 266100
  • 收稿日期:  2014-12-08
基金项目:

国家自然科学基金(41371314)资助.

摘要: 采用不同碳源,如油酸、菜籽油、棉籽油和煎炸废油作为假丝酵母菌(Candida. bombicola)ATCC 22214发酵生产槐糖脂的脂溶性碳源,考察了在不同碳源条件下该菌株合成槐糖脂的能力、所产槐糖脂的结构及表面活性特点.结果表明,以油酸、菜籽油及棉籽油为脂溶性碳源时,该菌株有着较高的槐糖脂产量,分别为101、73、51 g·L-1;通过高效液相色谱-高分辨质谱(HPLC-MS/MS)对以不同脂溶性碳源为发酵底物产生的槐糖脂进行分析,发现所产槐糖脂均由乙酰基取代的内酯型和酸型槐糖脂同系物构成且以内酯型槐糖脂结构为主.槐糖内酯中脂肪酸基的结构有所差异,但主要以脂肪链不饱和度为1的十八烯酸(C18:1)结构为主.所产槐糖脂具有较低的表面张力和临界胶束浓度(CMC),其中最低表面张力结果一致约36.0—37.0 mN·m-1,而CMC有所差异.同时,该槐糖脂具有良好的稳定性,其水溶液的最低表面张力在100 ℃下保持2 h基本不变,在0—20%的NaCl盐溶液及pH值为2—10范围内均保持良好的表面活性.

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