胞外多聚物在氧化亚铁硫杆菌吸附 Mn2+、Ni2+、Pb2+中的作用
The role of extracellular polymeric substances of Acidithiobacillus ferrooxidans on the adsorption of Mn2+, Ni2+ and Pb2+
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摘要: 利用从粤北大宝山酸性矿山废水(AMD)分离的氧化亚铁硫杆菌(A.f菌),采用原子吸收分光光度技术和荧光分光光度技术,研究其胞外多聚物(EPS)对水中Mn2+、Ni2+、Pb2+的吸附率及二者间的相互作用方式.结果表明,A.f菌对Mn2+、Ni2+、Pb2+具有较强的耐受性;A.f菌的EPS对3种金属离子吸附率随时间的延长而升高,当EPS吸附Mn2+、Ni2+、Pb2+后,类蛋白复合物荧光强度增强且最大发射峰位蓝移;A.f菌对Mn2+、Ni2+、Pb2+的耐受性与其胞外有机多聚物特别是类蛋白物质的生物吸附密切相关.A.f菌的EPS的生物吸附在水中重金属的去除中可发挥重要作用.
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关键词:
- Acidithiobacillus ferrooxidans /
- 锰 /
- 镍 /
- 铅 /
- 胞外多聚物
Abstract: A strain of Acidithiobacillus ferrooxidans (A.f) isolated from the acid mine drainage (AMD) of Dabaoshan Mine in North Guangdong was used to evaluate the adsorption rate of the extracellular polymeric substances (EPS) produced by the strain with Mn2+,Ni2+,Pb2+ and the interaction between EPS and Mn2+,Ni2+,Pb2+ using atomic absorption spectrophotometry and fluorescence spectrophotometry. The results indicated that the A. f showed a high resistance to Mn2+, Ni2+ and Pb2+. The adsorption rate of Mn2+, Ni2+, Pb2+ with EPS increased in a time-dependent way. The fluorescence intensity of the complex between of EPS and Mn2+, Ni2+, Pb2+ increased and the λmax had a blue-shit. The resistance of A.f to Mn2+, Ni2+ and Pb2+ is closely related with the biological adsorption of EPS, especially the extracellular proteoid. The biological adsorption of the metals by EPS might play an important role in the removal of heavy metals from water environments.-
Key words:
- Acidithiobacillus ferrooxidans /
- Mn /
- Ni /
- Pb /
- extracellular polymeric substances
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