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pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响

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璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
引用本文: 璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu
QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
Citation: QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu

pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响

  • 1.

    天津大学环境科学与工程学院, 天津, 300072

  • 基金项目:

    天津市应用基础研究计划项目(07JCZDJC02100)资助.

pH activated sludge sedimentation performance and the structure of the microbe of SBR technology

  • 1.

    School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China

  • Fund Project: Supported by Applied Basic Research Project of Tianjin(07JCZDJC02100).

  • 摘要: 本文主要研究了不同pH值对SBR工艺污泥膨胀和微生物结构的影响,试验进水pH值为5.0-10.0.实验结果表明,在试验初期,污泥容积指数(SVI)值无明显变化.但随着时间的增长,在pH值为5.0和6.0时,开始出现污泥膨胀.在不同pH下提取总细菌的DNA组进行PCR扩增,分析总细菌的Shannon多样性指数,对微生物群落的部分优势总细菌进行克隆测序和系统发育树分析.结果表明,在不同pH条件下微生物结构以及数量有所区别,在酸性条件下以丝状菌为主,易发生污泥膨胀;而在中性和偏碱性条件下以菌胶团为主,污泥活性较为稳定.
    Abstract: This paper mainly studied the influence of pH on SBR sludge bulking and microbial structure with the pH values of the inflow water from 5.0 to 10.0. The results showed that the did not change initially of Sludge Volume Index (SVI) value. However, the sludge bulking was seen over time when pH values became 5.0 and 6.0. At various pH values, the total bacterial DNA groups were extracted for PCR amplification, and then relevant analyses were given about Shannon diversity index, cloned sequencing and phylogenetic tree of partial dominant total bacteria in the microbial communities. This study demonstrated that the structures and quantities of microorganisms were different at different pH values:in acidic conditions filamentous bacteria became dominant and they were more accessible to sludge bulking in neutral and alkaline condition while zoogloea were the dominant ones and the activity of sludge was relatively stable.
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  • [1] NOVAK L, LARREA L, WARNER J, et al. Non-filamentous activated sludge bulking in a laboratory scale system[J]. Water research, 1993, 27(8):1339-1346.
    [2] 雷中方.高浓度钠盐对废水生物处理系统的失稳影响综述[J].工业水处理, 2000,20(4):6-10.

    LEI ZH F. The summarize of high concentration of sodium salt influence waster biological treatment system instability[J]. Industrial water treatment,2000,20(4):6-10(in Chinese).

    [3] 陈谊,孙宝盛, 张斌,等.不同MBR反应器中硝化菌群落结构的研究[J].中国环境科学,2010,30(1):69-75.

    CHEN Y, SUN B SH, ZHANG B, et al. The study of Nitrifying bacteria community structure in different MBR reactors[J].China Environment Science, 2010,30(1):69-75(in Chinese).

    [4] ZHOU J, MARY B, JAMES M T. DNA recovery from soils of diverse composition[J]. Applied and Environmental Microbiology, 1996,62(2):316-322.
    [5] GERARD MUYZER, WAAL E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16S rRNA[J]. Applied and Environmental Microbiology, 1993, 59(3):695-700.
    [6]
    [7] NICOLAISCN M H, RAMSING N B. Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria[J].Journal of Microbiologic-al Methods, 2002, 50(2):189-203.
    [8] ROB M R, JAN F M, LISE Y M. Assessment of activated sludge stability in lab-scal eexperiments[J].Journal of Biotechnology,2009, 141(3/4):147-154.
    [9] 朱哲,李涛,王东升,等.pH对活性污泥表面特性和形态结构的影响[J].环境工程学报,2008, 2(12):1599-1604.

    ZHU Z, LI T, WANG D S H, et al. Surface properties and morphology structure of activated sludge is influenced by pH[J].Journal of Environmental Engineering,2008,2(12):1599-06(in Chinese).

    [10] DAIMS H, LUCKER S, WAGNER M. Daime a novel image analysis program for microbial ecology and biofilm research[J]. Environmental Microbiology, 2006, 8(2):200-213
    [11] 王晓慧,文湘华.MAR-FISH技术及其在环境微生物群落与功能研究中的应用[J].微生物学通报,2009, 36(1):142-148.

    WANG X H, Wen X H. MBR-FISH technology and its application in environmental microbial community and function research[J].Microbiology Journal,2009, 36(1):142-148(in Chinese).

    [12] LEE N, NIELSEN P H, ANDREASEN K H, et al. Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology[J]. Applied and Environmental Microbiology, 1999, 65(3):1289-1297.
    [13] 余志晟,齐嵘,张洪勋.丝状细菌污泥膨胀的FISH探针研究进展[J].应用与环境生物学报,2012, 18(4):705-712.

    YU ZH SH, QI R, ZHANG H X. FISH probe research development of filamentous bacteria sludge expansion[J]. Journal of Application and Environment Biology,2012,18(4):705-712(in Chinese).

    [14] BRADFORD D, HUGENHOLTZ P, SEVIOUR E M, et al.16S rRNA analysis of isolates obtained from gram-negative, filament-tous bacteria micromanipulated from activated sludge[J]. Systematic and Applied Microbilogy, 1996, 19(3):334-343.
    [15] XIA Y, KONG Y H, NIELSEN P H. In situ detection of protein-hydrolysing microorganisms in activated sludge[J]. Fems Microbiology Ecology, 2007, 60(1):156-165.
    [16] KRAGELUND C, LEVANTESI C, BORGER A, et al. Identity abundance and ecophysiology of filamentous Chloroflexi species present in activated sludge treatment plants[J]. Fems Microbiology Ecology, 2007, 59(3):671-682.
    [17] SCHADE M, BEIMFOHR C, LEMMER H. Phylogenetic and physiological characterization of a "Nostocoida limicola"-like organism isolated from activated sludge[J]. Water Science and Technology, 2002, 46(1/2):91-97.
    [18] NIELSEN P H, KRAGELUND C, SEVIOUR R J, et al. Identity and ecophysiology of filamentous bacteria in activated sludge[J]. Fems Microbiology Reviews. 2009, 33(6):969-998.
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  • 收稿日期:  2015-07-31
  • 刊出日期:  2016-03-15
璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
引用本文: 璩绍雷, 孙宝盛, 赵双红, 臧向荣, 李志静, 魏佳虹. pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响[J]. 环境化学, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu
QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
Citation: QU Shaolei, SUN Baosheng, ZHAO Shuanghong, ZANG Xiangrong, LI Zhijing, WEI Jiahong. pH activated sludge sedimentation performance and the structure of the microbe of SBR technology[J]. Environmental Chemistry, 2016, 35(3): 508-515. doi: 10.7524/j.issn.0254-6108.2016.03.2015073101
shu

pH对间歇进水序批式生物反应(SBR)工艺活性污泥沉降性能和微生物结构的影响

  • 1. 天津大学环境科学与工程学院, 天津, 300072
  • 收稿日期:  2015-07-31
基金项目:

天津市应用基础研究计划项目(07JCZDJC02100)资助.

摘要: 本文主要研究了不同pH值对SBR工艺污泥膨胀和微生物结构的影响,试验进水pH值为5.0-10.0.实验结果表明,在试验初期,污泥容积指数(SVI)值无明显变化.但随着时间的增长,在pH值为5.0和6.0时,开始出现污泥膨胀.在不同pH下提取总细菌的DNA组进行PCR扩增,分析总细菌的Shannon多样性指数,对微生物群落的部分优势总细菌进行克隆测序和系统发育树分析.结果表明,在不同pH条件下微生物结构以及数量有所区别,在酸性条件下以丝状菌为主,易发生污泥膨胀;而在中性和偏碱性条件下以菌胶团为主,污泥活性较为稳定.

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