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污泥减量菌剂及其水解污泥条件优化

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汪顺丽, 查君茹, 张宏才, 魏荷芬, 胡子全, 赵海泉. 污泥减量菌剂及其水解污泥条件优化[J]. 环境化学, 2016, 35(4): 817-825. doi: 10.7524/j.issn.0254-6108.2016.04.2015110202
引用本文: 汪顺丽, 查君茹, 张宏才, 魏荷芬, 胡子全, 赵海泉. 污泥减量菌剂及其水解污泥条件优化[J]. 环境化学, 2016, 35(4): 817-825. doi: 10.7524/j.issn.0254-6108.2016.04.2015110202
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WANG Shunli, ZHA Junru, ZHANG Hongcai, WEI Hefen, HU Ziquan, ZHAO Haiquan. Compound sludge-disintegration bacteria agent and the optimization of culture conditions[J]. Environmental Chemistry, 2016, 35(4): 817-825. doi: 10.7524/j.issn.0254-6108.2016.04.2015110202
Citation: WANG Shunli, ZHA Junru, ZHANG Hongcai, WEI Hefen, HU Ziquan, ZHAO Haiquan. Compound sludge-disintegration bacteria agent and the optimization of culture conditions[J]. Environmental Chemistry, 2016, 35(4): 817-825. doi: 10.7524/j.issn.0254-6108.2016.04.2015110202
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污泥减量菌剂及其水解污泥条件优化

  • 1.

    安徽农业大学生命科学学院, 合肥, 230036;

  • 2.

    安徽全民环保科技有限公司, 合肥, 230036

  • 基金项目:

    安徽省省级环保科研课题项目(11008727)资助.

Compound sludge-disintegration bacteria agent and the optimization of culture conditions

  • 1.

    Life Science College, Anhui Agriculture University, Hefei, 230036, China;

  • 2.

    Anhui Quanmin Environmental Technology Co., Ltd., Hefei, 230036, China

  • Fund Project: Supported by the Environmental Protection Scientific Research Foundation of Anhui Province (11008727).

  • 摘要: 为获取高效降解污泥胞外聚合物的优势菌种以用于最大程度使污泥减量化,从合肥望塘污水厂活性污泥中筛选3株具有高效代谢淀粉酶和蛋白酶的菌株SL-10、SL-11和SL-16.通过形态特征、16S rRNA gene序列分析和系统发育分析,菌株SL-10、SL-11和SL-16分别鉴定为枯草芽孢杆菌属(Bacillus subtilis)、解淀粉芽孢杆菌属(Bacillus amyloliquefaciens)、巨大芽孢杆菌属(Bacillus megaterium).以污泥SCOD为指标,通过正交实验确定污泥减量菌剂各菌株最佳配比为SL-10:SL-11:SL-16=3:2:1,菌剂培养条件优化实验确定最佳培养时间为32 h,最佳培养温度为35 ℃,发酵培养基最适初始pH值为6.0,发酵最适装液量为80 mL/250 mL.优化污泥减量菌剂培养条件后用于处理污泥,确定处理时间为150 min,此时SCOD为841.19 mg·L-1,VSS为2316.54 mg·L-1,VSS溶解率为24.51%.
    Abstract: To obtain predominant strains with efficient degradation activity of extracellular polymeric substances (EPS) for sludge reduction, 3 strains of SL-10, SL-11, SL-16 with the amylase and protease activity were isolated from Wangtang sewage treatment plant in Hefei. The strains were identified as Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus megaterium respectively according to the traditional morphological,physiological and 16S rRNA gene sequence similarity analysis against GenBank. The optimum ratio of Compound Sludge-disintegration Bacteria Agent was determined as SL-10:SL-11:SL-16=3:2:1 by orthogonal test. The best culture conditions of the Compound Sludge-disintegration Bacteria Agent were investigated. The results indicated that the optimum culture conditions of the strain were as follow: time 32 h, temperature 35 ℃, pH 6.0, medium volume 80 mL/250 mL. Under the optimized conditions of compound sludge-disintegration bacteria agent, the soluble chemical oxygen demanded (SCOD) of WAS reached 841.19 mg·L-1 after 150 min treatment, and the volatile suspended solids (VSS) reached the minimum value of 2316.54 mg·L-1,with a removal efficiency of 24.51%.
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  • 收稿日期:  2015-11-02
  • 刊出日期:  2016-04-15

污泥减量菌剂及其水解污泥条件优化

  • 1.  安徽农业大学生命科学学院, 合肥, 230036;
  • 2.  安徽全民环保科技有限公司, 合肥, 230036
  • 收稿日期:  2015-11-02
基金项目:

安徽省省级环保科研课题项目(11008727)资助.

摘要: 为获取高效降解污泥胞外聚合物的优势菌种以用于最大程度使污泥减量化,从合肥望塘污水厂活性污泥中筛选3株具有高效代谢淀粉酶和蛋白酶的菌株SL-10、SL-11和SL-16.通过形态特征、16S rRNA gene序列分析和系统发育分析,菌株SL-10、SL-11和SL-16分别鉴定为枯草芽孢杆菌属(Bacillus subtilis)、解淀粉芽孢杆菌属(Bacillus amyloliquefaciens)、巨大芽孢杆菌属(Bacillus megaterium).以污泥SCOD为指标,通过正交实验确定污泥减量菌剂各菌株最佳配比为SL-10:SL-11:SL-16=3:2:1,菌剂培养条件优化实验确定最佳培养时间为32 h,最佳培养温度为35 ℃,发酵培养基最适初始pH值为6.0,发酵最适装液量为80 mL/250 mL.优化污泥减量菌剂培养条件后用于处理污泥,确定处理时间为150 min,此时SCOD为841.19 mg·L-1,VSS为2316.54 mg·L-1,VSS溶解率为24.51%.

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