采用电雾式检测器测定掺假蜂蜜中的果葡糖浆
Determination of fructose-glucose syrup in adulterated honey by HPLC-CAD
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摘要: 采用电雾式检测器建立了鉴别掺假蜂蜜的方法,实验采用乙腈和水(65:35)流动相等度洗脱,流速1.2 mL·min-1,Shodex Asahipak NH2P-50 4E色谱柱(4.6×150 mm,5 μm)进行分离,Corona Veo RS检测器采集频率5 Hz,蒸发温度35℃,Filter5.不同聚合度的麦芽糖在8.0-60.0 μg·mL-1浓度范围,线性相关系数为0.999;方法灵敏度高,1%果葡糖浆可明显检出.方法快捷高效,可用于掺假蜂蜜的快速鉴别.Abstract: A HPLC-CAD Method was established for rapidly quantification of oligosaccharides adulterated honey. We use a Shodex Asahipak NH2P-50 4E column(4.6×150 mm, 5 μ m)to seperate these compounds, with the mobile phase consisting of acetonitrile and H2O (65:35). The samples were detected by Corona Veo RS detector, with collect rate 5Hz,evaporative temperature 35℃,Filter 5. As a result, using the chromatographic peaks of maltooligosaccharide (maltose to maltoheptaose) standards as reference, the differences of honey, fructose-glucose syrup and pure honey containing fructose-glucose syrup were detected by CAD. The linear ranges of these different malto oligosaccharides were 8.0-60.0 mg·L-1 and the correlation coefficients(r) were all 0.999. 1% fructose-glucose syrup in adulterated honey can be detected, this method showed high accuracy, sensitivity and short time for analysis.
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Key words:
- HPLC-CAD /
- adulterated honey /
- fructose-glucose syrup /
- maltose
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