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不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响

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韩枫, 刘轶群, 王琴, 黄振武. 不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响[J]. 环境化学, 2019, 38(4): 957-960.
引用本文: 韩枫, 刘轶群, 王琴, 黄振武. 不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响[J]. 环境化学, 2019, 38(4): 957-960.
shu
HAN Feng, LIU Yiqun, WANG Qin, HUANG Zhenwu. Effects on chromium and selenium in blood of rats to different serine intakes[J]. Environmental Chemistry, 2019, 38(4): 957-960.
Citation: HAN Feng, LIU Yiqun, WANG Qin, HUANG Zhenwu. Effects on chromium and selenium in blood of rats to different serine intakes[J]. Environmental Chemistry, 2019, 38(4): 957-960.
shu

不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响

  • 1.

    中国疾病预防控制中心营养与健康所, 北京, 100050

  • 基金项目:

    中国疾病预防控制中心营养与健康所青年基金（NINH20160010）资助.

Effects on chromium and selenium in blood of rats to different serine intakes

  • 1.

    National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China

  • Fund Project: Supported by the Science Foundation for Young Scientists of National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention (NINH20160010).

  • 摘要: 本文研究了丝氨酸摄入水平对大鼠血液中铬和硒水平的影响.孕期wistar大鼠适应性饲养后，按体重随机分为4组.对照组和干预A组给予正常基础饲料；干预B组和C组给予缺丝氨酸和甘氨酸饲料；干预A组和C组在分组后腹腔注射PHGDH抑制剂.孕鼠生产后进行腹主动脉取血后处死，测定并比较各组血液中Cr和Se的含量.从对照组孕鼠产下的健康仔鼠中随机选取10只仔鼠，继续给予正常基础饲料，作为对照组.从D组孕鼠产下的健康仔鼠中随机选取40只，并随机分为4组（SD组、SD+L组、SD+M组、SD+H组），每组10只.SD+L组、SD+M组、SD+H组继续给予缺丝氨酸和甘氨酸饲料，饲养一个月后，这三组开始每日灌胃丝氨酸，剂量分别为：0.76 mg/100 g bw、1.52 mg/100 g bw和 3.04 mg/100 g bw.丝氨酸补充两个月后，对仔鼠进行腹主动脉取血后处死，测定并比较各组血液中Cr和Se的含量.结果：各组孕鼠之间的血Cr水平无统计学差异（P>0.05）.与对照组比较，干预B组和干预C组孕鼠血硒含量显著升高.与干预A组的孕鼠比较，干预C组的孕鼠血硒含量同样显著升高.血液中Cr水平与Se水平呈正相关（PPPP>0.05），而SD+M和SD+H组仔鼠的血硒水平则显著增加（PPPP>0.05）.仔鼠血液中Cr水平与Se含量无显著相关性（P>0.05）. 丝氨酸的摄入水平可以不同程度影响体内铬和硒的含量.
    Abstract: The effects on chromium (Cr) and selenium (Se) in blood of rats to different serine intakes was studied. After adapting for 5 days, pregnant rats sere randomly divided into 4 groups:control group, A, B and C group. Basic feed was given in control group and A group, meanwhile serine and glycine deficiency feed was given in B and C group. PHGDH inhibitor was intraperitoneal injected into A and C group. The chromium and selenium contents in pregnant rats were determined by inductively coupled plasma mass spectrometry (ICP-MS). Offspring rats from control group pregant rats were collected as control group. Offspring rats of C group were divided into 4 groups:SD group, SD+L group, SD+M group and SD+H group. Control group rats were feeded with basic food. SD group, SD+L group, SD+M group and SD+H group were given serine and glycine deficiency feed. After 1 months, 0.76 mg/100 g bw,1.52 mg/100 g bw and 3.04 mg/100 g bw serine were given to SD+L group, SD+M group and SD+H group, repectively. The chromium and selenium contents in offspring rats were determined by ICP-MS. There is no significant difference for the Cr contents betweent different groups. As the level of serine and glycine intakes decreased, the levels of Se in blood were increased. A positive relationship between blood Se and Cr content was found in pregnant rats, and not observed in offspring rats. For offsping rats, as the serine intakes increased, the Se and Cr content were increased. Conclusion the blood Cr and Se level were effected by the serine intakes.
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  • [1] HEIDEN M G V, THOMPSON C B. Understanding the warburg effect:The metabolic requirements of cell proliferation[J]. Science, 2009, 324(5930):1029.
    [2] FEDIRKO V, LUKANOVA A, BAMIA C, et al. Glycemic index, glycemic load, dietaiy carbohydrate, and dietary fiber intake and risk of liver and biliary tract cancers in Western Europeans[J]. Annals of Oncology, 2013, 24(2):543-553.
    [3] KRYUKOV G V, CASTELLANO S, NOVOSELOV S V, et al. Characterization of mammalian selenoproteomes[J]. Science, 2003, 300:1439-1443.
    [4] WANG Q, SUN LC, LIU YQ, et al. The Synergistic effect of serine with selenocompounds on the expression of SelP and GPx in HepG2 Cells[J]. Biol Trace Elem Res,2016,173(2):291-296.
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  • 刊出日期:  2019-04-15
韩枫, 刘轶群, 王琴, 黄振武. 不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响[J]. 环境化学, 2019, 38(4): 957-960.
引用本文: 韩枫, 刘轶群, 王琴, 黄振武. 不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响[J]. 环境化学, 2019, 38(4): 957-960.
shu
HAN Feng, LIU Yiqun, WANG Qin, HUANG Zhenwu. Effects on chromium and selenium in blood of rats to different serine intakes[J]. Environmental Chemistry, 2019, 38(4): 957-960.
Citation: HAN Feng, LIU Yiqun, WANG Qin, HUANG Zhenwu. Effects on chromium and selenium in blood of rats to different serine intakes[J]. Environmental Chemistry, 2019, 38(4): 957-960.
shu

不同丝氨酸摄入水平对大鼠血液中微量元素含量的影响

  • 1. 中国疾病预防控制中心营养与健康所, 北京, 100050
基金项目:

中国疾病预防控制中心营养与健康所青年基金（NINH20160010）资助.

摘要: 本文研究了丝氨酸摄入水平对大鼠血液中铬和硒水平的影响.孕期wistar大鼠适应性饲养后，按体重随机分为4组.对照组和干预A组给予正常基础饲料；干预B组和C组给予缺丝氨酸和甘氨酸饲料；干预A组和C组在分组后腹腔注射PHGDH抑制剂.孕鼠生产后进行腹主动脉取血后处死，测定并比较各组血液中Cr和Se的含量.从对照组孕鼠产下的健康仔鼠中随机选取10只仔鼠，继续给予正常基础饲料，作为对照组.从D组孕鼠产下的健康仔鼠中随机选取40只，并随机分为4组（SD组、SD+L组、SD+M组、SD+H组），每组10只.SD+L组、SD+M组、SD+H组继续给予缺丝氨酸和甘氨酸饲料，饲养一个月后，这三组开始每日灌胃丝氨酸，剂量分别为：0.76 mg/100 g bw、1.52 mg/100 g bw和 3.04 mg/100 g bw.丝氨酸补充两个月后，对仔鼠进行腹主动脉取血后处死，测定并比较各组血液中Cr和Se的含量.结果：各组孕鼠之间的血Cr水平无统计学差异（P>0.05）.与对照组比较，干预B组和干预C组孕鼠血硒含量显著升高.与干预A组的孕鼠比较，干预C组的孕鼠血硒含量同样显著升高.血液中Cr水平与Se水平呈正相关（PPPP>0.05），而SD+M和SD+H组仔鼠的血硒水平则显著增加（PPPP>0.05）.仔鼠血液中Cr水平与Se含量无显著相关性（P>0.05）. 丝氨酸的摄入水平可以不同程度影响体内铬和硒的含量.

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