槐糖脂生物表面活性剂的结构特征及理化性质初探

宋丹丹; 梁生康; 王江涛

环境化学

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宋丹丹, 梁生康, 王江涛. 槐糖脂生物表面活性剂的结构特征及理化性质初探[J]. 环境化学, 2011, 30(8): 1474-1479.

引用本文:

宋丹丹, 梁生康, 王江涛. 槐糖脂生物表面活性剂的结构特征及理化性质初探[J]. 环境化学, 2011, 30(8): 1474-1479.

SONG Dandan, LIANG Shengkang, WANG Jiangtao. STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS[J]. Environmental Chemistry, 2011, 30(8): 1474-1479.

Citation:

SONG Dandan, LIANG Shengkang, WANG Jiangtao. STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS[J]. Environmental Chemistry, 2011, 30(8): 1474-1479.

槐糖脂生物表面活性剂的结构特征及理化性质初探

1.
中国海洋大学化学化工学院, 青岛, 266100;
2.
中国海洋大学海洋化学理论与工程技术教育部重点实验室, 青岛, 266100
基金项目:
山东省自然科学基金项目(Y2008B28)

中国海洋大学与中海石油环保服务有限公司攻关项目(2006004)资助.

STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS

1.
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, China;
2.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao, 266100, China
Fund Project:

摘要: 通过摇瓶培养实验,考察了假丝酵母菌(Candida bombicola)ATCC 22214以葡萄糖和油酸双底物为碳源,采用一次加料培养和分批补料培养方式进行发酵制备槐糖脂的过程,并利用高效液相色谱/电喷雾飞行时间质谱联用仪确定了所产槐糖脂的结构,初步研究了其主要的理化性质.结果表明,一次加料培养中,该菌株生长稳定期的中期即120 h时槐糖脂产率最高,达45 g·L-1,分批补料培养方式中,196 h时糖脂产率达到101g·L-1.所产糖脂由7种乙酰基取代的含有17-羟基十八烯酸或十八烷酸的内酯型和酸型槐糖脂同系物构成,其中,保留时间为45.46 min的17-L--十八烯酸-1',4″-内酯-6',6″二乙酸酯和保留时间48.35 min的17-L--十八烷酸-1',4″-内酯-6',6″二乙酸酯比例较高,分别达27.38%和21.53%.该生物表面活性剂具有良好的表面活性和乳化能力,临界胶束浓度较低,为36.5 mg·L-1.
- 槐糖脂 /
- 发酵 /
- 高效液相色谱-电喷雾飞行时间质谱 /
- 表面张力 /
- 乳化性能
Abstract: The process of sophorolipids biosynthesis by yeast Candida bombicola ATCC 22214 from the mixture of glucose and oleic acid was evaluated by batch fermentation and fed-batch fermentation in the shake Erlenmeyer flask. The chemical composition of the sophorolipids mixture was identified by high performance liquid chromatography (HPLC) method combined with positive ion electrospray-time of flight-mass spectrometry (ESI-TOF/MS). The yield of the biosurfactant reached its maximu of 45 g·L-1 in the middle of the steady phase of the strain growth (i.e. 120 h) in batch culture, and it reached 101 g·L-1 in fed-batch culture. The biosurfactant mixture contained seven homologues of acetylated lactonic and acid sophorolipids with saturated and unsaturated C18 fatty acid moieties. The content of two major components, 17-L-[(2'-O-β-D- glucopyranosyl-β-D-glucopyranosyl)-oxy-]-octadecenoic acid -1',4″-lactone-6',6″-diactate and 17-L- [(2'-O-β-D-glucopyranosyl-β-D-glucopyranosyl)-oxy-]-octadecanoic acid -1',4″-lactone- 6',6″- diactate was 27.38% and 21.53%, respectively. The sophorolipid biosurfactant exhibited high surface-activity and good emulsification capacity and a low critical micelle concentration of 36.5 mg·L-1.
- sophorolipid /
- fermentation /
- high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry /
- surface tension /
- emulsification activities

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宋丹丹, 梁生康, 王江涛. 槐糖脂生物表面活性剂的结构特征及理化性质初探[J]. 环境化学, 2011, 30(8): 1474-1479.

引用本文:

宋丹丹, 梁生康, 王江涛. 槐糖脂生物表面活性剂的结构特征及理化性质初探[J]. 环境化学, 2011, 30(8): 1474-1479.

SONG Dandan, LIANG Shengkang, WANG Jiangtao. STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS[J]. Environmental Chemistry, 2011, 30(8): 1474-1479.

Citation:

SONG Dandan, LIANG Shengkang, WANG Jiangtao. STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS[J]. Environmental Chemistry, 2011, 30(8): 1474-1479.

槐糖脂生物表面活性剂的结构特征及理化性质初探

1. 中国海洋大学化学化工学院, 青岛, 266100;
2. 中国海洋大学海洋化学理论与工程技术教育部重点实验室, 青岛, 266100

收稿日期: 2010-10-10

基金项目:

山东省自然科学基金项目(Y2008B28)

中国海洋大学与中海石油环保服务有限公司攻关项目(2006004)资助.

关键词:

摘要: 通过摇瓶培养实验,考察了假丝酵母菌(Candida bombicola)ATCC 22214以葡萄糖和油酸双底物为碳源,采用一次加料培养和分批补料培养方式进行发酵制备槐糖脂的过程,并利用高效液相色谱/电喷雾飞行时间质谱联用仪确定了所产槐糖脂的结构,初步研究了其主要的理化性质.结果表明,一次加料培养中,该菌株生长稳定期的中期即120 h时槐糖脂产率最高,达45 g·L-1,分批补料培养方式中,196 h时糖脂产率达到101g·L-1.所产糖脂由7种乙酰基取代的含有17-羟基十八烯酸或十八烷酸的内酯型和酸型槐糖脂同系物构成,其中,保留时间为45.46 min的17-L--十八烯酸-1',4″-内酯-6',6″二乙酸酯和保留时间48.35 min的17-L--十八烷酸-1',4″-内酯-6',6″二乙酸酯比例较高,分别达27.38%和21.53%.该生物表面活性剂具有良好的表面活性和乳化能力,临界胶束浓度较低,为36.5 mg·L-1.

English Abstract

STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS

1. College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, China;
2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao, 266100, China

Received Date: 2010-10-10

Fund Project:

Keywords:

sophorolipid /
fermentation /
high performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry /
surface tension /
emulsification activities

Abstract: The process of sophorolipids biosynthesis by yeast Candida bombicola ATCC 22214 from the mixture of glucose and oleic acid was evaluated by batch fermentation and fed-batch fermentation in the shake Erlenmeyer flask. The chemical composition of the sophorolipids mixture was identified by high performance liquid chromatography (HPLC) method combined with positive ion electrospray-time of flight-mass spectrometry (ESI-TOF/MS). The yield of the biosurfactant reached its maximu of 45 g·L-1 in the middle of the steady phase of the strain growth (i.e. 120 h) in batch culture, and it reached 101 g·L-1 in fed-batch culture. The biosurfactant mixture contained seven homologues of acetylated lactonic and acid sophorolipids with saturated and unsaturated C18 fatty acid moieties. The content of two major components, 17-L-[(2'-O-β-D- glucopyranosyl-β-D-glucopyranosyl)-oxy-]-octadecenoic acid -1',4″-lactone-6',6″-diactate and 17-L- [(2'-O-β-D-glucopyranosyl-β-D-glucopyranosyl)-oxy-]-octadecanoic acid -1',4″-lactone- 6',6″- diactate was 27.38% and 21.53%, respectively. The sophorolipid biosurfactant exhibited high surface-activity and good emulsification capacity and a low critical micelle concentration of 36.5 mg·L-1.

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槐糖脂生物表面活性剂的结构特征及理化性质初探

STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS

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槐糖脂生物表面活性剂的结构特征及理化性质初探

English Abstract

STRUCTURE CHARACTERIZATION AND PHYSI-CHEMICAL PROPERTIES OF SOPHOROLIPID BIOSURFACTANTS

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