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不同磷条件下高温胁迫对铜绿微囊藻增殖的影响及其恢复

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李婷, 景元书, 韩玮. 不同磷条件下高温胁迫对铜绿微囊藻增殖的影响及其恢复[J]. 环境工程学报, 2015, 9(10): 4780-4788. doi: 10.12030/j.cjee.20151026
引用本文: 李婷, 景元书, 韩玮. 不同磷条件下高温胁迫对铜绿微囊藻增殖的影响及其恢复[J]. 环境工程学报, 2015, 9(10): 4780-4788. doi: 10.12030/j.cjee.20151026
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Li Ting, Jing Yuanshu, Han Wei. Effect of high temperature on cell proliferation and recovery of Microcystis aeruginosa at different initial phosphate concentrations[J]. Chinese Journal of Environmental Engineering, 2015, 9(10): 4780-4788. doi: 10.12030/j.cjee.20151026
Citation: Li Ting, Jing Yuanshu, Han Wei. Effect of high temperature on cell proliferation and recovery of Microcystis aeruginosa at different initial phosphate concentrations[J]. Chinese Journal of Environmental Engineering, 2015, 9(10): 4780-4788. doi: 10.12030/j.cjee.20151026
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不同磷条件下高温胁迫对铜绿微囊藻增殖的影响及其恢复

  • 1.

    气象灾害预报预警与评估协同创新中心, 南京 210044

  • 2.

    南京信息工程大学应用气象学院, 南京 210044

  • 3.

    河北省气象灾害防御中心, 石家庄 050021

  • 4.

    江苏省农业气象重点实验室, 南京 210044

  • 基金项目:

    江苏省科技支撑项目(BE2011840)

    江苏省农业气象重点实验室开放基金资助项目(JKLAM201205)

    第九批"六大人才高峰"高层次人才项目(NY-038)

    江苏高校优势学科建设工程项目(PAPD)

  • 中图分类号: X524

Effect of high temperature on cell proliferation and recovery of Microcystis aeruginosa at different initial phosphate concentrations

  • 1.

    Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Nanjing 210044, China

  • 2.

    College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China

  • 3.

    Hebei Province Meteorological Disaster Prevention Centre, Shijiazhuang 050021, China

  • 4.

    Jiangsu Key Laboratory of Agricultural Meteorology, Nanjing 210044, China

  • Fund Project:

  • 摘要: 为研究磷浓度与高温对铜绿微囊藻(Microcystis aeruginosa)细胞增殖及其恢复生长的影响,对低磷(0.05 mg/L)、中磷(0.50 mg/L)和高磷(5.00 mg/L)3种不同磷浓度下,35℃高温胁迫3、6和12 d后的生长过程进行了研究。以25℃为对照,测定了细胞密度、叶绿素a、胞内可溶性蛋白和糖含量、酶活性等指标。结果表明,低磷和中磷条件下,35℃高温未成为限制条件,反而促进细胞增殖。随磷浓度的增加,35℃高温的抑制作用增强,高磷条件下,温度成为限制因子,抑制藻的生长,第12天细胞密度仅为对照的85.51%。解除胁迫后,低、中磷条件下前期胁迫时间越长,藻细胞密度和叶绿素a含量越高,高磷条件下的细胞密度发生低补偿,胁迫3、6和12 d组比对照降低了3.44%、2.85%和10.32%。高磷组的各指标数值较高,组间差距相对较小,磷营养充足有利于微囊藻的补偿生长。不同磷营养下微囊藻会发生程度不同的补偿效应,可根据其自身变化为蓝藻生长的研究提供基本资料。
    Abstract: The cell proliferation and recovery growth after high temperature stress were discussed in this paper,with Microcystis aeruginosa as the experimental material.Two groups of 35℃ and 25℃ cultures were set at three different initial phosphate concentrations -0.05 mg/L(low-P),0.50 mg/L(medium-P) and 5.00 mg/L(high-P).They were cultured for 3,6,and 12 days before transferred to normal conditions under the same inoculation density.The algae under 25℃ all the time was set as the control.Cell density,chlorophyll-a,intracellular protein,carbohydrate and antioxidant system activities were measured during the course.The results showed that under the high temperature stress,the growth of M.aeruginosa in low and medium phosphate was promoted,without the heat inhibition.In high phosphate,it was inhibited significantly with a ratio of cell density being 85.51% of the control.The higher the phosphate content,the stronger the inhibition of temperature stress.After the relief of stress,in low and medium phosphate,the longer the stress,the higher the cell density and chlorophyll-a content.The cell density of 35℃ for 3,6,12 days groups in high phosphate were 3.44%,2.85% and 10.32% lower than those of the control,respectively,showing under-compensation.The indexes in high phosphate were higher than those in low and medium phosphate,and showed lower difference among groups,indicating that adequate phosphate was conductive to recovery.Different levels of compensation appeared in different phosphate nutrition.According to the dynamic change of biochemical characteristics,basic theoretical support of algae growth was provided in this paper.
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  • 收稿日期:  2014-08-04
  • 刊出日期:  2015-10-14

不同磷条件下高温胁迫对铜绿微囊藻增殖的影响及其恢复

  • 1.  气象灾害预报预警与评估协同创新中心, 南京 210044
  • 2.  南京信息工程大学应用气象学院, 南京 210044
  • 3.  河北省气象灾害防御中心, 石家庄 050021
  • 4.  江苏省农业气象重点实验室, 南京 210044
  • 收稿日期:  2014-08-04
基金项目:

江苏省科技支撑项目(BE2011840)

江苏省农业气象重点实验室开放基金资助项目(JKLAM201205)

第九批"六大人才高峰"高层次人才项目(NY-038)

江苏高校优势学科建设工程项目(PAPD)

摘要: 为研究磷浓度与高温对铜绿微囊藻(Microcystis aeruginosa)细胞增殖及其恢复生长的影响,对低磷(0.05 mg/L)、中磷(0.50 mg/L)和高磷(5.00 mg/L)3种不同磷浓度下,35℃高温胁迫3、6和12 d后的生长过程进行了研究。以25℃为对照,测定了细胞密度、叶绿素a、胞内可溶性蛋白和糖含量、酶活性等指标。结果表明,低磷和中磷条件下,35℃高温未成为限制条件,反而促进细胞增殖。随磷浓度的增加,35℃高温的抑制作用增强,高磷条件下,温度成为限制因子,抑制藻的生长,第12天细胞密度仅为对照的85.51%。解除胁迫后,低、中磷条件下前期胁迫时间越长,藻细胞密度和叶绿素a含量越高,高磷条件下的细胞密度发生低补偿,胁迫3、6和12 d组比对照降低了3.44%、2.85%和10.32%。高磷组的各指标数值较高,组间差距相对较小,磷营养充足有利于微囊藻的补偿生长。不同磷营养下微囊藻会发生程度不同的补偿效应,可根据其自身变化为蓝藻生长的研究提供基本资料。

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