壳寡糖对镉致肝脏损伤的保护作用及机理研究
Chitooligosaccharide Alleviation on Cadmium-induced Liver Injury
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摘要: 为研究壳寡糖（chitooligosaccharide，COS）对镉致肝脏损伤的作用及机制，选用32只体质量在16~19 g的健康雌性C57BL/6小鼠，随机分为4个组：空白对照组（Control）、氯化镉造模组（Cd）、壳寡糖组（COS）、壳寡糖+氯化镉组（COS+Cd）。空白对照组和氯化镉造模组每日饲喂基础日粮，饮用纯净水；壳寡糖组和壳寡糖+氯化镉组每日饲喂基础日粮，在饮水中添加0.3 g·L-1 COS。于第28天对氯化镉造模组和壳寡糖+氯化镉组小鼠采取腹腔注射5.45 mg·kg-1氯化镉的处理，12 h后采样。结果显示：（1）添加COS缓解了重金属镉累积导致的肝细胞颗粒变性、细胞结构模糊等病理症状；（2） COS缓解了由于重金属镉暴露导致的氧化应激，激活了小鼠体内的氧化型谷胱甘肽（oxidized glutathione，GSSG）/谷胱甘肽（glutathione，GSH）系统（P<0.05），显著减少了肝脏由于氧化损伤而产生的活性氧（reactive oxygen species，ROS）（P<0.05），有利于缓解肝脏由于重金属富集而导致的肝脏氧化损伤；（3）镉暴露使小鼠肝脏细胞中Nrf2的水平显著升高（P<0.01），提高了机体应激信号通路关键分子p38 MAPK的磷酸化水平（P<0.05），同时抑制了关键炎症信号分子p-NF-кB p65（P<0.05）。综上可知，COS可通过调控p38 MAPK/NF-кB p65/Nrf2等关键因子的表达缓解镉对肝脏的氧化损伤，从而达到对肝脏的保护作用。Abstract: This research aims to study the effect and mechanism of chitooligosaccharide (COS) on alleviating liver injury induced by cadmium. A total of 32 healthy female C57BL/6 mice with a body weight of 16 to 19 g were randomly divided into four groups:Control group (Control), cadmium chloride model group (Cd), COS group (COS), and COS+cadmium chloride group (COS+Cd). Control group and cadmium chloride model group were fed basal diet and pure water every day. Mice in the COS group and the COS+Cd group were fed a basal diet and pure water with 0.3 g·L-1 COS. On the 28th day of the experiment, mice in the Cd group and the COS+Cd group were intraperitoneally injected with 5.45 mg·kg-1 CdCl2 and sampled 12 h later. The results showed that:(1) COS supplement alleviated the pathological symptoms of liver cell granule degeneration, and repaired the cellular structure caused by cadmium induction. (2) COS supplement alleviated the oxidative stress caused by cadmium induction, activated the oxidized glutathione (GSSG)/glutathione (GSH) system in mice (P<0.05), and significantly reduced the reactive oxygen species (ROS) in the liver (P<0.05). It is beneficial to alleviate liver damage caused by heavy metal exposure. (3) Cd induction significantly increased the relative level of Nrf2 in the liver (P<0.01), activated the relative expression of phosphorylated p38 MAPK, a key molecule of stress signaling pathway (P<0.05), and inhibited the phosphorylated NF-кB p65 (P<0.05). In conclusion, COS supplement could alleviate oxidative damage in the liver induced by cadmium via regulating the expression of key factors, such as p38 MAPK/NF-кB p65/Nrf2, then achieving a protective effect.
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Key words:
- cadmium /
- liver cells of mouse /
- acute toxicity /
- chitooligosaccharide /
- oxidative stress
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