硫酸铜暴露对牛蛙心缩能力及神经冲动传导影响的研究
Effects of CuSO4 Exposure on Bullfrog Heart Activity and Its Nerve Conduction Velocity
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摘要: 铜离子作为工业废水中最常见的重金属离子，对两栖动物的生存及发育有重要的影响。为研究工业废水中重金属Cu2+对两栖动物的毒害作用，本试验采用不同浓度CuSO4对美国牛蛙的离体蛙心以及坐骨神经干进行处理。通过以浓度梯度为0.01、0.1、1.0、10.0和100.0 mg·L-1的CuSO4溶液进行蛙心灌流和神经干浸泡，使用生物机能实验系统测定离体蛙心肌张力和心率的变化，以及坐骨神经干动作电位传导速度的变化。试验结果表明，美国牛蛙暴露于较低浓度的CuSO4溶液（0.01 mg·L-1）中时心缩张力即显著降低（P<0.01）；在较低浓度的Cu2+影响下，心率呈现出上升的趋势，暴露于1.0 mg·L-1 CuSO4中时心率达到最大值（109.73±13.54）次·min-1，显著高于对照组（P<0.01），再增加CuSO4浓度，心率逐渐降低，但依旧显著高于对照组（P<0.01），在实验暴露剂量下没有观察到显著性改变；牛蛙坐骨神经干经较低浓度CuSO4溶液处理后其传导速度呈现出上升的趋势，当暴露于0.1 mg·L-1 CuSO4溶液中时出现了最大值（7.56±1.29） m·s-1，再增大CuSO4溶液的浓度，传导速度显著降低，当暴露于1.0 mg·L-1 CuSO4溶液中时即表现出了明显的抑制作用（P<0.01）。本研究发现当牛蛙暴露于不同浓度的CuSO4溶液中时，各种生理特性均呈现出明显的变化，整体呈现出抑制作用，可为工业废水中Cu2+的排放标准的制定提供数据参考。Abstract: As the most common heavy metal ion in industrial wastewater, Cu2+ has adverse effects on the survival and development of amphibians. In order to examine the toxic effects of heavy metal Cu2+ exposure on amphibians. Different concentrations of CuSO4 were used to poison the American bullfrog's heart and the sciatic nerve trunk. The CuSO4 solutions with a concentration gradient of 0.01, 0.1, 1.0, 10.0, 100.0 mg·L-1 were used for perusing the bullfrog's heart and soaking the nerve trunk. The biological function experimental system was used to determine the heart tension and rate, as well as the sciatic nerve action potential conduction velocity. The results showed that the systolic tension decreased (P<0.01) when the bullfrog was exposed to low concentration CuSO4 solution (0.01 mg·L-1). The beating rate of the heart showed an upward trend with the gradual increase of CuSO4 concentration and reached the maximum (109.73±13.54) order·min-1 at 1.0 mg·L-1 CuSO4 (P<0.01). With a further increase of CuSO4 concentration, the heart rate gradually decreased, but it was still higher than control group (P<0.01). Similarly, the conduction velocity of bullfrog sciatic nerve trunk showed an upward trend when exposed to low concentration of CuSO4 solutions, and reached the maximum value (7.56±1.29) m·s-1 at 0.1 mg·L-1 CuSO4, and thereafter the value decreased with a further increase of the concentration. Our results suggest that exposure to various concentrations of industrial wastewater containing CuSO4 would adversely affect various physiological characteristics of bullfrog, which thus can provide reference for formulation and revision of Cu2+ discharge standard.
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Key words:
- CuSO4 /
- isolated bullfrog heart /
- contraction tension /
- heart rate /
- nerve conduction velocity
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