氯虫苯甲酰胺及其代谢物IN-EQW78对蚯蚓氧化应激及繁殖能力的影响
Effects of Chlorantraniliprole and Its Metabolite IN-EQW78 on Antioxidant and Reproductive Capacity of Earthworms
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摘要: 为明确氯虫苯甲酰胺(CAP)及其代谢物氯虫苯甲酰胺脱水环化物(IN-EQW78)对蚯蚓毒性效应的差异，开展了CAP和代谢物IN-EQW78对蚯蚓的56 d单一暴露实验，探讨2种化合物分别对蚯蚓氧化应激及繁殖能力的影响。结果表明，5 mg·kg-1的CAP暴露14 d后，过氧化氢酶(CAT)活性显著上升，超氧化物歧化酶(SOD)活性在28 d时明显增强；IN-EQW78暴露7 d后，SOD和谷胱甘肽转移酶(GSH-ST)活性增强，而CAT活性降低。IN-EQW78比CAP会更早地引起蚯蚓氧化应激效应，并引发更大的氧化损伤。5 mg·kg-1的CAP处理减少了蚯蚓精子数量，破坏卵巢结构，对生殖系统造成损伤，并进一步抑制蚯蚓繁殖，导致56 d时幼蚓数量显著下降；而IN-EQW78(≥0.04 mg·kg-1)处理对卵巢无损伤，反而引起蚯蚓精子数量增加，从而促进蚯蚓繁殖，显著提高了56 d时幼蚓及蚓茧数量。本研究揭示了CAP及其代谢物对蚯蚓的潜在影响，为农业生产中CAP的风险评估提供了科学依据。Abstract: In this study, earthworms (Eisenia fetida) were singly exposed to chlorantraniliprole (CAP) and its metabolite (chlorantraniliprole dehydrated cyanide, IN-EQW78) for 56 days to investigate the toxic effects on the antioxidant system and reproduction ability of earthworms, aiming to indicate the distinctions between CAP and IN-EQW78. The results showed that the catalase (CAT) and superoxide dismutase (SOD) activities significantly increased after exposure to 5 mg·kg-1 CAP for 14 d and 28 d, respectively. After exposure to IN-EQW78 for 7 d, the activities of SOD and glutathione transferase (GSH-ST) were enhanced, while CAT activity was inhibited. Compared with CAP, IN-EQW78 induced oxidative stress in the earlier stage and triggered greater oxidative damage. In addition, exposure to 5 mg·kg-1 CAP reduced the number of earthworm spermatozoa, destroyed the structure of ovary, caused damage to the reproductive system, and further inhibited the reproduction of earthworms, resulting in a significant decrease in the number of juvenile earthworms at 56 d. By contrast, exposure to IN-EQW78 (≥0.04 mg·kg-1) induced the increase of the number of spermatozoa without any damage to the ovary, thereby facilitating the reproduction of the earthworms, and significantly enhanced the number of juvenile earthworms and cocoon numbers at 56 d. This study revealed the potential effects of CAP and its metabolites on earthworms, and provided a scientific basis for risk assessment of CAP in agricultural production.
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Key words:
- chlorantraniliprole /
- IN-EQW78 /
- earthworms (Eisenia fetida) /
- oxidative damage /
- reproductive toxicity
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