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藻毒素降解菌CQ5对MC-LR粗提液的降解动力学

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陈泽慧, 高志伟, 董小娜, 苏鹏, 毛林强, 张文艺. 藻毒素降解菌CQ5对MC-LR粗提液的降解动力学[J]. 环境化学, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
引用本文: 陈泽慧, 高志伟, 董小娜, 苏鹏, 毛林强, 张文艺. 藻毒素降解菌CQ5对MC-LR粗提液的降解动力学[J]. 环境化学, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
shu
CHEN Zehui, GAO Zhiwei, DONG Xiaona, SU Peng, MAO Linqiang, ZHANG Wenyi. Degradation kinetics of MC-LR crude extracts by strain CQ5[J]. Environmental Chemistry, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
Citation: CHEN Zehui, GAO Zhiwei, DONG Xiaona, SU Peng, MAO Linqiang, ZHANG Wenyi. Degradation kinetics of MC-LR crude extracts by strain CQ5[J]. Environmental Chemistry, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
shu

藻毒素降解菌CQ5对MC-LR粗提液的降解动力学

  • 1.

    常州大学环境与安全工程学院, 常州, 213164;

  • 2.

    常州市天宁区郑陆镇工业和科技环保服务中心, 常州, 213000

  • 基金项目:

    国家自然科学基金（41571471），江苏省和常州市科技支撑项目（BE2016653，WS201621）资助.

Degradation kinetics of MC-LR crude extracts by strain CQ5

  • 1.

    School of Environmental & Safety Engineering,Changzhou University,Changzhou, 213164, China;

  • 2.

    Zhenglu Industrial and Technology Environmental Protection Service Center, Tianning District, Changzhou, 213000, China

  • Fund Project: Supported by the National Natural Science Foundation of China (41571471), Science and Technology Project of Jiangsu Province and Changzhou City(CE20155061, WS201621).

  • 摘要: 针对近年来采用微生物法降解水体中的微囊藻毒素（microcystins，MCs）这一研究热点问题，以本课题组前期从太湖芦苇荡底泥中筛出的耐硼赖氨酸芽孢杆菌CQ5（Lysinibacillus boronitolerans）为考察对象，研究微生物对MC-LR的降解动力学.分别采用Logistic生长方程和Monod动力学方程构建菌株CQ5细胞生长动力学模型和MC-LR降解动力学模型.结果表明，菌株CQ5在以MC-LR粗提液为碳、氮源的无机盐培养基中的生长曲线符合Logistic生长模型，其中菌株生长环境承载量K为1.306，菌株生长平均速率r为0.1685，无量纲参数a为1.688；该菌株在6 d内可使MC-LR的浓度由14.12 μg·L-1降至1.57 μg·L-1，降解率达88.86%，其一级反应速率常数k为0.3698，半衰期t1/2为1.88 d；该降解过程中MC-LR浓度、菌株细胞密度和MC-LR降解速率3者间的偶合关系符合低浓度下的Monod模型，其中υmax/Ks为0.342；一级反应动力学方程式S=e2.648-0.3698t和Monod模型方程式S=14.12e-0.342Nt（N=1.08）均可模拟预测降解体系中的MC-LR浓度，二者的模拟结果高度一致.本文可为研究微生物降解MC-LR的机理和推动MC-LR降解菌的工程应用提供理论参考.
    Abstract: Microcystin-LR (MC-LR) is the most common and studied variant of microcystins (MCs), which have drawn increasing environmental concerns worldwide. In this study, a bacterial strain CQ5 was investigated from the sediments sampled from Taihu Lake and the biodegradation kinetics of MC-LR by strain CQ5 were investigated. Logistic growth model and Monod kinetics model were used to establish the CQ5 cell growth kinetics model and MC-LR degradation kinetics model, respectively. The results showed that strain CQ5 was capable of utilizing MC-LR as carton and nitrogen source for growth. The growth curve of strain CQ5 fitted with the Logistic growth model, where K was 1.306, r was 0.1685 and the dimensionless parameter a was 1.688. The concentration of MC-LR was reduced from 14.12 μg·L-1 to 1.57 μg·L-1 and the degradation rate was 88.86% within 6 days. The degradation process agreed with the first-order reaction kinetics equation, where k was 0.3698 and half-time t1/2 was 1.88 d. The linkage relationship between MC-LR concentration, cell density and MC-LR degradation rate was consistent with the Monod model at low concentration, where Vmax/Ks was 0.342. The first-order reaction kinetics equation was S=e2.648-0.3698t and the Monod model equation was S=14.12e-0.342Nt(N=1.08). They could be employed to simulate and predict the growth of strain CQ5 and the MC-LR concentration in degradation system. And their predicted results were highly consistent. These results could provide theoretical reference for studying the mechanism of MC-LR biodegradation and promoting the engineering application of bacteria which could degrade MC-LR.
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  • 收稿日期:  2017-06-04
  • 刊出日期:  2018-01-15
陈泽慧, 高志伟, 董小娜, 苏鹏, 毛林强, 张文艺. 藻毒素降解菌CQ5对MC-LR粗提液的降解动力学[J]. 环境化学, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
引用本文: 陈泽慧, 高志伟, 董小娜, 苏鹏, 毛林强, 张文艺. 藻毒素降解菌CQ5对MC-LR粗提液的降解动力学[J]. 环境化学, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
shu
CHEN Zehui, GAO Zhiwei, DONG Xiaona, SU Peng, MAO Linqiang, ZHANG Wenyi. Degradation kinetics of MC-LR crude extracts by strain CQ5[J]. Environmental Chemistry, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
Citation: CHEN Zehui, GAO Zhiwei, DONG Xiaona, SU Peng, MAO Linqiang, ZHANG Wenyi. Degradation kinetics of MC-LR crude extracts by strain CQ5[J]. Environmental Chemistry, 2018, 37(1): 82-88. doi: 10.7524/j.issn.0254-6108.2017060403
shu

藻毒素降解菌CQ5对MC-LR粗提液的降解动力学

  • 1.  常州大学环境与安全工程学院, 常州, 213164;
  • 2.  常州市天宁区郑陆镇工业和科技环保服务中心, 常州, 213000
  • 收稿日期:  2017-06-04
基金项目:

国家自然科学基金（41571471），江苏省和常州市科技支撑项目（BE2016653，WS201621）资助.

摘要: 针对近年来采用微生物法降解水体中的微囊藻毒素（microcystins，MCs）这一研究热点问题，以本课题组前期从太湖芦苇荡底泥中筛出的耐硼赖氨酸芽孢杆菌CQ5（Lysinibacillus boronitolerans）为考察对象，研究微生物对MC-LR的降解动力学.分别采用Logistic生长方程和Monod动力学方程构建菌株CQ5细胞生长动力学模型和MC-LR降解动力学模型.结果表明，菌株CQ5在以MC-LR粗提液为碳、氮源的无机盐培养基中的生长曲线符合Logistic生长模型，其中菌株生长环境承载量K为1.306，菌株生长平均速率r为0.1685，无量纲参数a为1.688；该菌株在6 d内可使MC-LR的浓度由14.12 μg·L-1降至1.57 μg·L-1，降解率达88.86%，其一级反应速率常数k为0.3698，半衰期t1/2为1.88 d；该降解过程中MC-LR浓度、菌株细胞密度和MC-LR降解速率3者间的偶合关系符合低浓度下的Monod模型，其中υmax/Ks为0.342；一级反应动力学方程式S=e2.648-0.3698t和Monod模型方程式S=14.12e-0.342Nt（N=1.08）均可模拟预测降解体系中的MC-LR浓度，二者的模拟结果高度一致.本文可为研究微生物降解MC-LR的机理和推动MC-LR降解菌的工程应用提供理论参考.

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