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高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

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徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
引用本文: 徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
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XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
Citation: XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu

高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

  • 1.

    浙江省气象科学研究所, 杭州, 310008;

  • 2.

    宁波诺丁汉大学化学与环境工程系, 宁波, 315100

  • 基金项目:

    国家重点研发计划（2016YFC0201900），国家自然科学基金（41303091，91544226），浙江省公益技术研究社会发展项目（2015C33011）和宁波市室内空气污染净化技术创新团队（2017C510001）资助.

Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry

  • 1.

    Zhejiang Institute of Meteorological Sciences, Hangzhou, 310008, China;

  • 2.

    Department of Chemical and Environmental Engineering, The University of Nottingham Ningbo China, Ningbo, 315100, China

  • Fund Project: Supported by the National Key R&D Program of China(2016YFC0201900), the National Natural Science Foundation of China (41303091,91544226), Nonprofit Technology Applied Research Project of the Zhejiang Province(2015C33011) and Ningbo Innovation Team on Indoor Air Pollution Control Technology (2017C510001).

  • 摘要: 为实现真菌气溶胶示踪物的准确、快速、高灵敏测定，通过高效液相色谱和三重四极杆质谱联用，优化相关分析参数，建立了对阿糖醇和甘露醇这2种目标有机示踪物无需衍生化处理即可进行直接检测的快速分析方法.结果显示，在流动相氨水的浓度0.0001%、流速选择0.4 mL·min-1、柱温箱温度45 ℃、负离子模式条件下，2种目标有机示踪物的线性范围是0.01—1 mg·L-1（R2>0.99）， 检测限分别达到5.6 μg·L-1（阿糖醇）、9.4 μg·L-1 （甘露醇）.该方法检测限低、灵敏度高、样品回收率高、重现性好，并具有较好的实用性.
    Abstract: An accurate, fast and sensitive analytical method for fungi aerosol tracers including both arabitol and mannitol has been developed using high performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) without any derivatization process, which is able to greatly reduce sample preparation time. The optimum analytical condition has been established as below:ammonium hydroxide (ω:0.0001%) as mobile phase with a flow rate of 0.4 mL·min-1, oven temperature of 45℃, and negative ion electrospray ionization. Under the abovementioned analytical condition, the linear range of the two organic tracers is 0.01-1 mg·L-1(R2>0.99) and limits of detection (LOD) are 5.6 μg·L-1(arabitol) and 9.4 μg·L-1 (mannitol), respectively. After validation and application to the real aerosol samples, this new method has been proved to be a reliable and cost-effective alternative technique for analysis of both arabitol and mannitol.
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    HU L F, ZHANG K, WANG H B, et al. Concentration and particle size distribution of microbiological aerosol during haze days in Beijing[J]. Environmental Science, 2015, 36(9):3144-3149(in Chinese).

    [6] 杜睿,周宇光. 北京及周边地区大气近地面层真菌气溶胶的变化特征[J]. 中国环境科学,2010, 30(3):296-301.

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  • 收稿日期:  2017-04-20
  • 刊出日期:  2017-12-15
徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
引用本文: 徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu
XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
Citation: XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu

高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

  • 1.  浙江省气象科学研究所, 杭州, 310008;
  • 2.  宁波诺丁汉大学化学与环境工程系, 宁波, 315100
  • 收稿日期:  2017-04-20
基金项目:

国家重点研发计划（2016YFC0201900），国家自然科学基金（41303091，91544226），浙江省公益技术研究社会发展项目（2015C33011）和宁波市室内空气污染净化技术创新团队（2017C510001）资助.

摘要: 为实现真菌气溶胶示踪物的准确、快速、高灵敏测定，通过高效液相色谱和三重四极杆质谱联用，优化相关分析参数，建立了对阿糖醇和甘露醇这2种目标有机示踪物无需衍生化处理即可进行直接检测的快速分析方法.结果显示，在流动相氨水的浓度0.0001%、流速选择0.4 mL·min-1、柱温箱温度45 ℃、负离子模式条件下，2种目标有机示踪物的线性范围是0.01—1 mg·L-1（R2>0.99）， 检测限分别达到5.6 μg·L-1（阿糖醇）、9.4 μg·L-1 （甘露醇）.该方法检测限低、灵敏度高、样品回收率高、重现性好，并具有较好的实用性.

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