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一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用

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王黎芸, 方迪, 周立祥. 一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用[J]. 环境化学, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
引用本文: 王黎芸, 方迪, 周立祥. 一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用[J]. 环境化学, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
shu
WANG Liyun, FANG Di, ZHOU Lixiang. Isolation and identification of a sulfur-reducing bacterium and its role in treatment of heavy metal-loaded acidic wastewater[J]. Environmental Chemistry, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
Citation: WANG Liyun, FANG Di, ZHOU Lixiang. Isolation and identification of a sulfur-reducing bacterium and its role in treatment of heavy metal-loaded acidic wastewater[J]. Environmental Chemistry, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
shu

一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用

  • 1.

    南京农业大学资源与环境科学学院, 南京, 210095

  • 基金项目:

    国家自然科学基金（21677077）资助

Isolation and identification of a sulfur-reducing bacterium and its role in treatment of heavy metal-loaded acidic wastewater

  • 1.

    College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

  • Fund Project: Supported by the National Natural Science Foundation of China (21677077)

  • 摘要: 采用Hungate厌氧滚管法，从酸性重金属废水中筛选了1株嗜酸性的硫单质还原菌NAU-16.经形态学和16S rDNA基因序列分析鉴定为脱硫菌属硫磺细菌种Desulfurella amilsii.研究了其在不同温度（20—60 ℃）、pH（1.0—7.0）和碳源（乙酸、丙醇、乳酸、葡萄糖、丙三醇、丙酮酸）条件下的生长特性，并通过序批式厌氧瓶培养考察了该菌株对污泥生物沥浸酸液中Zn2+、Cu2+、Ni2+的去除效果.结果表明，菌株NAU-16最适生长温度为35—45 ℃，可在pH值3.0—7.0范围内较好生长，能利用乙酸、葡萄糖作为电子供体和碳源.同时，Desulfurella amilsii NAU-16介导的生物硫单质还原可有效处理含Zn2+、Cu2+、Ni2+的污泥生物沥浸酸液.对于初始pH 3.0—4.0的沥浸液，处理12 d，Zn2+、Cu2+的去除率达99%以上，Ni2+的去除率90%—99%.上述研究结果为酸性重金属废水生物处理提供了一种新途径.
    Abstract: Strain NAU-16 was isolated from heavy metal-loaded acidic wastewater by the method of Hungate operation. According to its morphology and 16S rDNA gene sequencing, this strain was identified to be Desulfurella amilsii. Its growth property and sulfur reduction ability at different temperatures (20-60℃), initial pH (1.0-7.0) and electron donors (acetate, propionate, lactate, glucose, glycerol, pyruvate) were investigated. The role of this strain in removing heavy metals in acid wastewater containing Zn2+, Cu2+, Ni2+ were evaluated in sequencing batch. The results showed that strain NAU-16 growed at optimum temperatures from 35℃ to 45℃ and a broad pH range from 3.0 to 7.0. It was able to couple the oxidation of acetate and glucose to the reduction of elemental sulfur. Furthermore, this isolate-mediated biological sulfur reduction strain NAU-16 showed efficient removal of Zn2+, Cu2+, Ni2+. Up to 99% Zn2+, Cu2+ and up to 90%-99% Ni2+ were removed after 12 days, with initial pH 3.0 and 4.0 treatment. Strain NAU-16 could be a good candidate to cope with low pH and high metal concentration wastewater.
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  • 收稿日期:  2018-03-30
  • 刊出日期:  2019-02-15
王黎芸, 方迪, 周立祥. 一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用[J]. 环境化学, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
引用本文: 王黎芸, 方迪, 周立祥. 一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用[J]. 环境化学, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
shu
WANG Liyun, FANG Di, ZHOU Lixiang. Isolation and identification of a sulfur-reducing bacterium and its role in treatment of heavy metal-loaded acidic wastewater[J]. Environmental Chemistry, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
Citation: WANG Liyun, FANG Di, ZHOU Lixiang. Isolation and identification of a sulfur-reducing bacterium and its role in treatment of heavy metal-loaded acidic wastewater[J]. Environmental Chemistry, 2019, 38(2): 370-376. doi: 10.7524/j.issn.0254-6108.2018033002
shu

一株嗜酸硫单质还原菌的分离鉴定及其在酸性重金属废水处理中的作用

  • 1. 南京农业大学资源与环境科学学院, 南京, 210095
  • 收稿日期:  2018-03-30
基金项目:

国家自然科学基金（21677077）资助

摘要: 采用Hungate厌氧滚管法，从酸性重金属废水中筛选了1株嗜酸性的硫单质还原菌NAU-16.经形态学和16S rDNA基因序列分析鉴定为脱硫菌属硫磺细菌种Desulfurella amilsii.研究了其在不同温度（20—60 ℃）、pH（1.0—7.0）和碳源（乙酸、丙醇、乳酸、葡萄糖、丙三醇、丙酮酸）条件下的生长特性，并通过序批式厌氧瓶培养考察了该菌株对污泥生物沥浸酸液中Zn2+、Cu2+、Ni2+的去除效果.结果表明，菌株NAU-16最适生长温度为35—45 ℃，可在pH值3.0—7.0范围内较好生长，能利用乙酸、葡萄糖作为电子供体和碳源.同时，Desulfurella amilsii NAU-16介导的生物硫单质还原可有效处理含Zn2+、Cu2+、Ni2+的污泥生物沥浸酸液.对于初始pH 3.0—4.0的沥浸液，处理12 d，Zn2+、Cu2+的去除率达99%以上，Ni2+的去除率90%—99%.上述研究结果为酸性重金属废水生物处理提供了一种新途径.

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