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根瘤菌S2对离子态和酒石酸络合态铜的吸附行为

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张进, 彭焕龙, 徐海星, 叶坚, 徐梁, 李达, 李邦, 梁咏梅, 刘伟. 根瘤菌S2对离子态和酒石酸络合态铜的吸附行为[J]. 环境化学, 2019, 38(6): 1282-1290. doi: 10.7524/j.issn.0254-6108.2018080201
引用本文: 张进, 彭焕龙, 徐海星, 叶坚, 徐梁, 李达, 李邦, 梁咏梅, 刘伟. 根瘤菌S2对离子态和酒石酸络合态铜的吸附行为[J]. 环境化学, 2019, 38(6): 1282-1290. doi: 10.7524/j.issn.0254-6108.2018080201
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ZHANG Jin, PENG Huanlong, XU Haixing, YE Jian, XU Liang, LI Da, LI Bang, LIANG Yongmei, LIU Wei. Biosorption behavior of Rhizobium sp. S2 on ionic and tartaric acid chelated copper[J]. Environmental Chemistry, 2019, 38(6): 1282-1290. doi: 10.7524/j.issn.0254-6108.2018080201
Citation: ZHANG Jin, PENG Huanlong, XU Haixing, YE Jian, XU Liang, LI Da, LI Bang, LIANG Yongmei, LIU Wei. Biosorption behavior of Rhizobium sp. S2 on ionic and tartaric acid chelated copper[J]. Environmental Chemistry, 2019, 38(6): 1282-1290. doi: 10.7524/j.issn.0254-6108.2018080201
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根瘤菌S2对离子态和酒石酸络合态铜的吸附行为

  • 1.

    中山大学环境科学与工程学院, 广东省环境污染控制与修复技术重点实验室, 广州, 510006

  • 基金项目:

    广东省应用型科技研发专项资金（2015B020235006）和广东省水利科技创新项目（2017-20）资助.

Biosorption behavior of Rhizobium sp. S2 on ionic and tartaric acid chelated copper

  • 1.

    Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510006, China

  • Fund Project: Supported by Guangdong Provincial Special Science and Technology Fund for Practical Research and Development (2015B020235006) and Water Resource Science and Technology Innovation Program of Guangdong Province (2017-20).

  • 摘要: 探究根瘤菌S2的吸附动力学和热力学行为以及根据不同投菌量和pH的影响分析其对于Cu2+及酒石酸络合铜的吸附性能；结合对吸附前后上清液及菌体进行EEM、FTIR、XRD以及XPS分析解释菌株吸附机理.结果表明，菌株吸附2 mg·L-1 Cu2+及酒石酸络合铜均可分为快速吸附和慢速平衡阶段，12 h后基本达到平衡，吸附率达97%以上；温度的升高会导致吸附量增加.最适投菌量约为0.6 g·L-1 wet cells；菌株吸附Cu2+的最适pH值为6，而当pH值小于10时菌株对于酒石酸络合铜的吸附几乎不受影响.在吸附过程中，溶解性微生物产物（SMP）、细胞壁上的还原和官能团络合机制均发挥关键作用，吸附铜离子过程中SMP的作用更明显，而细胞壁在络合铜的吸附过程中的作用较大，其破络效率决定于细菌活性.
    Abstract: The biosorption kinetics, thermodynamic behaviors, and influence factors (microbial dosages and pH) experiments were conducted to investigate the biosorption capacity of ionic and tartaric acid chelated copper with Rhizobium sp. strain S2. EEM, FTIR, XRD and XPS were employed to characterized the solution and strain before and after biosorption. The results showed that the biosorption process of 2 mg·L-1 ionic and chelated copper involved rapid and slow biosorption stages and reached equilibrium after 12 h with the biosorption efficiency up to 97%. Higher temperature led to higher adsorption, and the optimum microbial dosage was approximately 0.6 g wet cells·L-1. The optimum pH of biosorption of ionic copper was 6, and the biosorption capacity of tartaric acid chelated copper was independent of pH below 10. The effects of SMP, reduction and complexation of cell wall were pivotal, the role of SMP was more significant in biosorption for ionic copper while cell wall played a greater role in biosorption for chelated copper.
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  • 收稿日期:  2018-08-02
  • 刊出日期:  2019-06-15

根瘤菌S2对离子态和酒石酸络合态铜的吸附行为

  • 1. 中山大学环境科学与工程学院, 广东省环境污染控制与修复技术重点实验室, 广州, 510006
  • 收稿日期:  2018-08-02
基金项目:

广东省应用型科技研发专项资金（2015B020235006）和广东省水利科技创新项目（2017-20）资助.

摘要: 探究根瘤菌S2的吸附动力学和热力学行为以及根据不同投菌量和pH的影响分析其对于Cu2+及酒石酸络合铜的吸附性能；结合对吸附前后上清液及菌体进行EEM、FTIR、XRD以及XPS分析解释菌株吸附机理.结果表明，菌株吸附2 mg·L-1 Cu2+及酒石酸络合铜均可分为快速吸附和慢速平衡阶段，12 h后基本达到平衡，吸附率达97%以上；温度的升高会导致吸附量增加.最适投菌量约为0.6 g·L-1 wet cells；菌株吸附Cu2+的最适pH值为6，而当pH值小于10时菌株对于酒石酸络合铜的吸附几乎不受影响.在吸附过程中，溶解性微生物产物（SMP）、细胞壁上的还原和官能团络合机制均发挥关键作用，吸附铜离子过程中SMP的作用更明显，而细胞壁在络合铜的吸附过程中的作用较大，其破络效率决定于细菌活性.

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