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生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地

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鲍根莲, 邓欢欢, 李少君, 马斌斌, 叶盛, 葛利云. 生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地[J]. 环境化学, 2019, (7): 1497-1506. doi: 10.7524/j.issn.0254-6108.2018092705
引用本文: 鲍根莲, 邓欢欢, 李少君, 马斌斌, 叶盛, 葛利云. 生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地[J]. 环境化学, 2019, (7): 1497-1506. doi: 10.7524/j.issn.0254-6108.2018092705
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BAO Genlian, DENG Huanhuan, LI Shaojun, MA Binbin, YE Sheng, GE Liyun. Biosurfactants generated from Marine Microbe and Chelator assisted remediation of Cadmium and Copper contaminated Saline-alkaline Soil by Ficus concinna var.Subsessilis[J]. Environmental Chemistry, 2019, (7): 1497-1506. doi: 10.7524/j.issn.0254-6108.2018092705
Citation: BAO Genlian, DENG Huanhuan, LI Shaojun, MA Binbin, YE Sheng, GE Liyun. Biosurfactants generated from Marine Microbe and Chelator assisted remediation of Cadmium and Copper contaminated Saline-alkaline Soil by Ficus concinna var.Subsessilis[J]. Environmental Chemistry, 2019, (7): 1497-1506. doi: 10.7524/j.issn.0254-6108.2018092705
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生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地

  • 1. 温州医科大学公共卫生与管理学院, 温州, 325035;

  • 2. 浙南水科学研究院, 温州, 325035;

  • 3. 上海海庭环境工程有限公司, 上海, 200092;

  • 4. 浙江伟明环保股份有限公司, 温州, 325088;

  • 5. 温州医科大学, 温州, 325035

    • 通讯作者: 葛利云, E-mail: 437720244@qq.com
  • 基金项目:

    浙江省自然科学基金（LY17E090006，LY14D060009，GF18C050005），温州市科技局项目（Y20170202）和浙江省医药卫生科技项目（2018271164）资助.

Biosurfactants generated from Marine Microbe and Chelator assisted remediation of Cadmium and Copper contaminated Saline-alkaline Soil by Ficus concinna var.Subsessilis

  • 1. College of Public Health and Management, Wenzhou Medical University, Wenzhou, 325035, China;

  • 2. Southern Zhejiang Water Research Institute, Wenzhou, 325035, China;

  • 3. Shanghai Haiting Environmental Engineering Co., Ltd., Shanghai, 200092, China;

  • 4. Zhejiang Weiming Environment Protection Co., Ltd., Wenzhou, 325088, China;

  • 5. Wenzhou Medical University, Wenzhou, 325035, China

    • Corresponding author: GE Liyun, 437720244@qq.com
  • Fund Project: Supported by the Natural Science Foundation of Zhejiang Province (LY17E090006, LY14D060009, GF18C050005), the Wenzhou Municipal Science and Technology Bureau (Y20170202) and Medical Health Science and Technology Project of Zhejiang Province (2018271164).

  • 摘要: 采用盆栽试验研究海洋细菌和酵母产生物表面活性剂、化学螯合剂对无柄小叶榕（Ficus concinna var.Subsessilis）修复盐碱地重金属Cd、Cu的强化效果.结果表明，强化试验下无柄小叶榕能耐受Cd、Cu胁迫正常生长，且体内重金属含量随生物表面活性剂投加浓度增大而升高，表现为根>地上部分；300 mg·kg-1细菌产生物表面活性剂强化下，根部Cd含量最大值为313 mg·kg-1，1 mmol·kg-1柠檬酸（CA）-300 mg·kg-1酵母产生物表面活性剂强化下，根部Cu最大含量为2156 mg·kg-1.强化剂添加下，能显著提高Cd、Cu在小叶榕体内的累积量，无柄小叶榕对土壤Cd、Cu的吸收富集能力显著提高，1 mmol·kg-1 CA-300 mg·kg-1酵母产生物表面活性剂强化下Cd的最大富集系数为（9.76±0.10），是对照组S1（1.1±0.02）的8.90倍，300 mg·kg-1酵母产生物表面活性剂单独强化下Cu的最大富集系数为（7.42±0.16），是S1（0.77±0.03）的9.60倍；无柄小叶榕向地上转移Cu的能力较弱，TF<1，对Cu的提取修复潜能有限；300 mg·kg-1细菌产生物表面活性剂强化下Cd的最大修复率为2.56%，是对照组S1的4.70倍，300 mg·kg-1细菌产生物表面活性剂-1 mmol·kg-1 EDTA联合强化下Cu的最大修复率为1.80%，为S1的3.30倍.综上，无柄小叶榕对重金属污染的盐碱地有良好的修复潜力，生物表面活性剂和化学螯合剂的添加可有效提高小叶榕对重金属Cd和Cu的吸收富集效率.
    Abstract: The strengthening effects of surfactants produced by marine bacteria and yeast and chemical chelators on the removal of heavy metals Cd and Cu in saline-alkaline soil by Ficus concinna var.subsessilis were studied in pot experiments. The results showed when adding Cd and Cu, Ficus concinna var.subsessilis was able to grow well, and the heavy metal content in the plants increased with the increasing addition of the biosurfactant, and the heavy metal content in the root was higher than in the shoot. With the enhancement of 300 mg·kg-1 bacterial surfactant, the maximum content of Cd in the root was 313 mg·kg-1, whereas with 1 mmol·kg-1 CA-300 mg·kg-1 yeast surfactant addition, and the maximum content of Cu in the root was 2156 mg·kg-1. The accumulation of Cd and Cu in Ficus concinna var.subsessilis was significantly increased by the addition of the fortifier, and the absorption and enrichment capacity of Ficus concinna var.subsessilis to Cd and Cu in the soil of was remarkably increased. The maximum enrichment coefficient of Cd under the enhancement of 1 mmol·kg-1 CA-300 mg·kg-1 yeast surfactant was (9.76±0.10), which was 8.90 times as many as the control group S1 (1.1±0.02). The maximum enrichment coefficient of Cu under the single enhancement of 300 mg·kg-1 yeast surfactant was (7.42±0.16), which was 9.60 times as many as the control group S1 (0.77±0.03). It was weak for Ficus concinna var.subsessilis to transfer Cu from the shoot to the upper parts, and TF was below to1, which showed a limited extraction and repair potential of Ficus concinna var.subsessilis for Cu. The maximum repair rate of Cd with the combined enhancement of 300 mg·kg-1 bacterial surfactant was 2.56%, which was 4.70 times as many as in the blank reaction system "S1", and the maximum repair rate of Cu with the combined enhancement of 300 mg·kg-1 bacterial surfactant-1 mmol·kg-1 EDTA was 1.80%, which was 3.30 times as many as in the blank reaction system "S1". Then, the Ficus concinna var.subsessilis has a better potential to remove the heavy metal in saline-alkali soil, and the addition of biological surfactant and chemical chelator can effectively improve the absorption and enrichment efficiency of heavy metal Cd and Cu in Ficus concinna var.subsessilis.
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  • 收稿日期:  2018-09-27

生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地

    通讯作者: 葛利云, E-mail: 437720244@qq.com
  • 1. 温州医科大学公共卫生与管理学院, 温州, 325035;
  • 2. 浙南水科学研究院, 温州, 325035;
  • 3. 上海海庭环境工程有限公司, 上海, 200092;
  • 4. 浙江伟明环保股份有限公司, 温州, 325088;
  • 5. 温州医科大学, 温州, 325035
  • 收稿日期:  2018-09-27
基金项目:

浙江省自然科学基金（LY17E090006，LY14D060009，GF18C050005），温州市科技局项目（Y20170202）和浙江省医药卫生科技项目（2018271164）资助.

摘要: 采用盆栽试验研究海洋细菌和酵母产生物表面活性剂、化学螯合剂对无柄小叶榕（Ficus concinna var.Subsessilis）修复盐碱地重金属Cd、Cu的强化效果.结果表明，强化试验下无柄小叶榕能耐受Cd、Cu胁迫正常生长，且体内重金属含量随生物表面活性剂投加浓度增大而升高，表现为根>地上部分；300 mg·kg-1细菌产生物表面活性剂强化下，根部Cd含量最大值为313 mg·kg-1，1 mmol·kg-1柠檬酸（CA）-300 mg·kg-1酵母产生物表面活性剂强化下，根部Cu最大含量为2156 mg·kg-1.强化剂添加下，能显著提高Cd、Cu在小叶榕体内的累积量，无柄小叶榕对土壤Cd、Cu的吸收富集能力显著提高，1 mmol·kg-1 CA-300 mg·kg-1酵母产生物表面活性剂强化下Cd的最大富集系数为（9.76±0.10），是对照组S1（1.1±0.02）的8.90倍，300 mg·kg-1酵母产生物表面活性剂单独强化下Cu的最大富集系数为（7.42±0.16），是S1（0.77±0.03）的9.60倍；无柄小叶榕向地上转移Cu的能力较弱，TF<1，对Cu的提取修复潜能有限；300 mg·kg-1细菌产生物表面活性剂强化下Cd的最大修复率为2.56%，是对照组S1的4.70倍，300 mg·kg-1细菌产生物表面活性剂-1 mmol·kg-1 EDTA联合强化下Cu的最大修复率为1.80%，为S1的3.30倍.综上，无柄小叶榕对重金属污染的盐碱地有良好的修复潜力，生物表面活性剂和化学螯合剂的添加可有效提高小叶榕对重金属Cd和Cu的吸收富集效率.

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