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邻苯二甲酸酯与蔗糖转化酶的互用机制

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高岩, 陈文晶, 刘万贵, 王志刚. 邻苯二甲酸酯与蔗糖转化酶的互用机制[J]. 环境化学, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
引用本文: 高岩, 陈文晶, 刘万贵, 王志刚. 邻苯二甲酸酯与蔗糖转化酶的互用机制[J]. 环境化学, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
shu
GAO Yan, CHEN Wenjing, LIU Wangui, WANG Zhigang. Interaction between phthalic acid esters and invertase[J]. Environmental Chemistry, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
Citation: GAO Yan, CHEN Wenjing, LIU Wangui, WANG Zhigang. Interaction between phthalic acid esters and invertase[J]. Environmental Chemistry, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
shu

邻苯二甲酸酯与蔗糖转化酶的互用机制

  • 1. 齐齐哈尔大学生命科学与农林学院, 齐齐哈尔, 161006;

  • 2. 齐齐哈尔市富拉尔基区农业推广站, 齐齐哈尔, 161041

    • 通讯作者: 王志刚, E-mail: wzg1980830@sina.com
  • 基金项目:

    齐齐哈尔大学教育科学研究项目（2017038）和国家大学生创新创业训练计划项目（201810232034）资助.

Interaction between phthalic acid esters and invertase

  • 1. School of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, 161006, China;

  • 2. Agricultural Technology Promotion Station of Fulaerji District, Qiqihar City, Qiqihar, 161041, China

    • Corresponding author: WANG Zhigang, wzg1980830@sina.com
  • Fund Project: Supported by Qiqihar University Education Science Research Project (2017038) and College Student Innovation and Entrepreneurship Training Program (201810232034).

  • 摘要: 本研究以邻苯二甲酸酯（phthalic acid esters，PAEs）和蔗糖转化酶为试验材料，采用同步荧光光谱、共振光散射光谱（RLS）、分子平均粒径、分子对接模拟、分子动力学模拟、酶活性和酶促反应动力学，探究PAEs与蔗糖转化酶的微观作用机制.结果表明，随着PAEs浓度的增加，蔗糖转化酶的RLS强度和分子平均粒径逐渐降低；PAEs主要通过疏水作用与蔗糖转化酶活性中心氨基酸残基结合，并通过氢键作用锁定结合取向；PAEs导致蔗糖转化酶的RMSD值降低；PAEs污染导致蔗糖转化酶酶活性显著降低（P<0.05），并使得其反应动力学参数Km和Vmax减小，属于反竞争性抑制机制.研究表明，PAEs与蔗糖转化酶形成PAEs-酶复合物，改变了蔗糖转化酶的分子构象，并通过反竞争性抑制机制抑制蔗糖转化酶的酶活性.
    Abstract: In this study, mechanisms of the interaction between phthalic acid esters (PAEs) and invertase were investigated using resonance light scattering spectroscopy (RLS), particle size measurement, molecular docking, molecular dynamics simulation, enzyme activity assay and reaction kinetic experiment. The results showed that the RLS intensity and average particle size of invertase were decreased gradually with the increase of PAEs concentration. PAEs mainly bound with the amino acid residues of the catalytic domain of invertase by hydrophobic force, and locked the binding orientation by hydrogen bonding. Furthermore, the RMSD of invertase decreased by PAEs. In addition, the enzyme activity of invertase was inhibited by PAEs and the reaction kinetic parameters, Km and Vmax decreased, which belonged to the anti-competitive inhibition mechanism. We concluded that PAEs interacted with invertase to form PAEs-enzyme complex by hydrophobic force and hydrogen bond, which changed the conformations of invertase, and then inhibited the enzyme activities by anti-competitive inhibition mechanism.
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  • [1] ZENG F, CUI K, FU J, et al. Biodegradability of di(2-Ethylhexyl) phthalate by pseudomonas fluorescens FS1[J]. Water Air & Soil Pollution, 2002, 140(1-4):297-305.
    [2] RAHMAN M, BRAZEL C S. The plasticizer market:An assessment of traditional plasticizers and research trends to meet new challenges[J]. Progress in Polymer Science, 2004, 29(12):1223-1248.
    [3] BLAIR J D, IKONOMOU M G, KELLY B C, et al. Ultra-trace determination of phthalate ester metabolites in seawater, sediments, and biota from an urbanized marine inlet by LC/ESI-MS/MS[J]. Environmental Science & Technology, 2009, 43(16):6262-6268.
    [4] CHEN C W, DONG C D. Distribution of phthalate esters in sediments of Kaohsiung Harbor, Taiwan[J]. Soil and Sediment Contamination:An International Juounal, 2013, 22(2):119-131.
    [5] WANG J, BO L, LI L, et al. Occurrence of phthalate esters in river sediments in areas with different land use patterns[J]. Science of the Total Environment, 2014, 500-501:113-119.
    [6] XU D, DENG X, FANG E, et al. Determination of 23 phthalic acid esters in food by liquid chromatography tandem mass spectrometry[J]. Journal of Chromatography A, 2014, 1324:49-56.
    [7] 李天宝, 刘炜, 王春利, 等. 邻苯二甲酸酯类增塑剂提取技术研究进展[J]. 化学分析计量, 2015, 24(4):99-102.

    LI T B, LIU W, WANG C L, et al. Research progress on extraction technology of phthalate ester plasticizer[J]. Chemical Analysis and Meterage, 2015, 24(4):99-102(in Chinese).

    [8] YANG H, XIE W, LIU Q, et al. Distribution of phthalate esters in topsoil:A case study in the Yellow River Delta, China[J]. Environmental Monitoring & Assessment, 2013, 185(10):8489-8500.
    [9] 俞锞, 李志邈, 万红建, 等. 高等植物蔗糖转化酶功能的研究进展[J]. 安徽农业科学, 2013, 41(33):12815-12818.

    YU K, LI Z M, WANG H J, et al. Advances in studies on the functions of invertase in higher plants[J]. Anhui Agricultural Science, 2013, 41(33):12815-12818(in Chinese).

    [10] 王连军. 高等植物中蔗糖转化酶的研究进展[J]. 安徽农业科学, 2014, 42(24):8108-8111.

    WANG L J. Advances in invertase research in higher plants[J]. Anhui Agricultural Science, 2014, 42(24):8108-8111(in Chinese).

    [11] 刘慧英, 朱祝军. 转化酶在高等植物蔗糖代谢中的作用研究进展[J]. 植物学通报, 2002, 19(6):666-674.

    LIU H Y, ZHU Z J. Progress in the role of invertase in sucrose metabolism in higher plants[J]. Botany Bulletin, 2002, 19(6):666-674(in Chinese).

    [12] 王俊刚, 赵婷婷, 杨本鹏, 等. 甘蔗脱毒健康种苗中蔗糖转化酶表达分析[J]. 江苏农业科学, 2017, 45(21):35-39.

    WANG J G, ZHAO T T, YANG B P, et al. Expression analysis of sucrose invertase in healthy seedlings of sugarcane detoxification[J]. Jiangsu Agricultural Sciences, 2017, 45(21):35-39(in Chinese).

    [13] 李欢可. 微生物有机肥对土壤改良及烟草钾素累积规律研究[D]. 长沙:湖南大学, 2013. LI H K. Study on soil improvement and potassium accumulation in tobacco by microbial organic fertilizer[D]. Changsha:Hunan University, 2013(in Chinese).
    [14] ZHANG R, ZHAO L, LIU R. Deciphering the toxicity of bisphenol a to Candida rugosa lipase through spectrophotometric methods[J]. Journal of Photochemistry & Photobiology B Biology, 2016, 163:40-46.
    [15] SOMAN S, CHACKO A S, PRASAD V S, et al. Self-assembly of oleylamine modified nano-fibrillated cellulose from areca husk fibers into giant vesicles[J]. Carbohydrate Polymers, 2018, 182:69-74.
    [16] TAN S, CHI Z, SHAN Y, et al. Interaction studies of polybrominated diphenyl ethers (PBDEs) with human serum albumin (HSA):Molecular docking investigations[J]. Environmental Toxicology & Pharmacology, 2017, 54:34-39.
    [17] WU Z, YI Z, DONG L, et al. Molecular simulation study of the specific combination between four kinds of phthalic acid esters and human serum albumin[J]. Environmental Toxicology & Pharmacology, 2016, 41:259-265.
    [18] ZHOU H, WANG C, DENG T, et al. Novel urushiol derivatives as HDAC8 inhibitors:Rational design, virtual screening, molecular docking and molecular dynamics studies[J]. Journal of Biomolecular Structure and Dynamics, 2018, 36(8):1966-1978.
    [19] 李静媛, 张莹, 姜楠楠, 等. 加热处理对蜂蜜中4种酶活性的影响[J]. 安徽农学通报, 2017, 23(7):20-22.

    LI J Y, ZHANG Y, JIANG N N, et al. Effect of heating treatment on activity of 4 enzymes in honey[J]. Anhui Agricultural Science Bulletin, 2017, 23(7):20-22(in Chinese).

    [20] SUN H, XIA Q, LIU R. Comparison of the binding of the dyes Sudan Ⅱ and Sudan IV to bovine hemoglobin[J]. Journal of Luminescence, 2014, 148(7):143-150.
    [21] ZHANG P, LAN P, MA Y, et al. Spectroscopic investigation on the interaction of Cr(VI) with bovine serum albumin[J]. Journal of Biochemical and Molecular Toxicology, 2012, 26(2):54-59.
    [22] ZHANG R, ZHAO L, LIU R. Deciphering the toxicity of bisphenol a to Candida rugosa lipase through spectrophotometric methods[J]. Journal of Photochemistry & Photobiology B Biology, 2016, 163:40-46.
    [23] LIU Y, CAO R, QIN P, et al. Assessing the potential toxic effect of one persistent organic pollutant:Non-covalent interaction of dicofol with the enzyme trypsin[J]. Spectrochim Acta A Mol Biomol Spectrosc, 2012, 89(4):210-215.
    [24] WU Z, YI Z, DONG L, et al. Molecular simulation study of the specific combination between four kinds of phthalic acid esters and human serum albumin[J]. Environmental Toxicology & Pharmacology, 2016, 41(8):259-265.
    [25] TAN S, CHI Z, SHAN Y, et al. Interaction studies of polybrominated diphenyl ethers (PBDEs) with human serum albumin (HSA):Molecular docking investigations[J]. Environmental Toxicology & Pharmacology, 2017, 54:34-39.
    [26] TAN S, WANG D, CHI Z, et al. Study on the interaction between typical phthalic acid esters (PAEs) and human haemoglobin (hHb) by molecular docking[J]. Environmental Toxicology and Pharmacology, 2017, 53:206-211.
    [27] LI J, LI J, JIAO Y, et al. Spectroscopic analysis and molecular modeling on the interaction of jatrorrhizine with human serum albumin (HSA)[J]. Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy, 2014, 118(2):48-54.
    [28] PAWEL S'ledz', CAFLISCH A. Protein structure-based drug design:from docking to molecular dynamics[J]. Current Opinion in Structural Biology, 2017, 48:93-102.
    [29] JANOWSKI P A, LIU C, DECKMAN J, et al. Molecular dynamics simulation of triclinic lysozyme in a crystal lattice[J]. Protein Science, 2015, 25(1):87-102.
    [30] ZHANG R, ZHAO L, LIU R. Deciphering the toxicity of bisphenol a to Candida rugosa lipase through spectrophotometric methods[J]. Journal of Photochemistry & Photobiology B Biology, 2016, 163, 40-46.
    [31] ZENG F, CUI K, XIE Z, et al. Distribution of phthalate esters in urban soils of Subtropical City[J]. Journal of Hazardous Materials, 2009, 164:1171-1178.
    [32] GARCIA J M, ROBERTSON M L. The future of plastics recycling[J]. Science, 2017, 358(6365):870-872.
    [33] MO C, CAI Q, TANG S, et al. Polycyclic aromatic hydrocarbons and phthalic acid esters in vegetables from nine farms of the Pearl River Delta[J]. Archives of Environmental Contamination and Toxicology, 2009, 56(2):181-189.
    [34] CRABBE M J C. Book review:Enzyme kinetics:a modern approach[J]. Computational Biology & Chemistry, 2003, 27(2):161-162.
    [35] 陈文晶, 王志刚, 徐伟慧, 等. 邻苯二甲酸酯污染对黑土转化酶与脲酶反应动力学的影响[J]. 浙江农业学报, 2018, 30(9):1555-1560.

    CHEN W J, WANG Z G, XU W H, et al. Effect of phthalate esters contamination on urease and invertase reaction kinetics of black soil[J]. Acta Agriculturae Zhejiangensis, 2018, 30(9):1555-1560(in Chinese).

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  • 收稿日期:  2019-03-09
高岩, 陈文晶, 刘万贵, 王志刚. 邻苯二甲酸酯与蔗糖转化酶的互用机制[J]. 环境化学, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
引用本文: 高岩, 陈文晶, 刘万贵, 王志刚. 邻苯二甲酸酯与蔗糖转化酶的互用机制[J]. 环境化学, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
shu
GAO Yan, CHEN Wenjing, LIU Wangui, WANG Zhigang. Interaction between phthalic acid esters and invertase[J]. Environmental Chemistry, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
Citation: GAO Yan, CHEN Wenjing, LIU Wangui, WANG Zhigang. Interaction between phthalic acid esters and invertase[J]. Environmental Chemistry, 2020, (2): 482-488. doi: 10.7524/j.issn.0254-6108.2019030901
shu

邻苯二甲酸酯与蔗糖转化酶的互用机制

    通讯作者: 王志刚, E-mail: wzg1980830@sina.com
  • 1. 齐齐哈尔大学生命科学与农林学院, 齐齐哈尔, 161006;
  • 2. 齐齐哈尔市富拉尔基区农业推广站, 齐齐哈尔, 161041
  • 收稿日期:  2019-03-09
  • 网络出版日期:  2020-02-26
基金项目:

齐齐哈尔大学教育科学研究项目（2017038）和国家大学生创新创业训练计划项目（201810232034）资助.

摘要: 本研究以邻苯二甲酸酯（phthalic acid esters，PAEs）和蔗糖转化酶为试验材料，采用同步荧光光谱、共振光散射光谱（RLS）、分子平均粒径、分子对接模拟、分子动力学模拟、酶活性和酶促反应动力学，探究PAEs与蔗糖转化酶的微观作用机制.结果表明，随着PAEs浓度的增加，蔗糖转化酶的RLS强度和分子平均粒径逐渐降低；PAEs主要通过疏水作用与蔗糖转化酶活性中心氨基酸残基结合，并通过氢键作用锁定结合取向；PAEs导致蔗糖转化酶的RMSD值降低；PAEs污染导致蔗糖转化酶酶活性显著降低（P<0.05），并使得其反应动力学参数Km和Vmax减小，属于反竞争性抑制机制.研究表明，PAEs与蔗糖转化酶形成PAEs-酶复合物，改变了蔗糖转化酶的分子构象，并通过反竞争性抑制机制抑制蔗糖转化酶的酶活性.

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