高级搜索

高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态

,  ,  ,  ,  ,  , 

downloadPDF

上一篇

下一篇

邵鹏威, 路国慧, 郑宇, 胡俊栋, 赵全升, 杨永亮, 刘崴. 高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态[J]. 环境化学, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
引用本文: 邵鹏威, 路国慧, 郑宇, 胡俊栋, 赵全升, 杨永亮, 刘崴. 高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态[J]. 环境化学, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
shu
SHAO Pengwei, LU Guohui, ZHENG Yu, HU Jundong, ZHAO Quansheng, YANG Yongliang, LIU Wei. Determination of selenium species in rice flour using high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Environmental Chemistry, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
Citation: SHAO Pengwei, LU Guohui, ZHENG Yu, HU Jundong, ZHAO Quansheng, YANG Yongliang, LIU Wei. Determination of selenium species in rice flour using high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Environmental Chemistry, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
shu

高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态

  • 1. 青岛大学环境科学与工程学院, 青岛, 266071;

  • 2. 国家地质实验测试中心, 北京, 100037

    • 通讯作者: 刘崴, E-mail: hattieliu@126.com
  • 基金项目:

    中国地质科学院基本科研业务费项目（YYWF201620）和中国地质大调查项目（DD20190589）资助.

Determination of selenium species in rice flour using high performance liquid chromatography-inductively coupled plasma mass spectrometry

  • 1. College of Environmental Sciences and Engineering, Qingdao University, Qingdao, 266071, China;

  • 2. National Research Center for Geo-Analysis(NRCGA), Beijing, 100037, China

    • Corresponding author: LIU Wei, hattieliu@126.com
  • Fund Project: Supported by the Research Fund of Chinese Academy of Geological Sciences (YYWF201620) and the Geological Survey Project of China (DD20190589).

  • 摘要: 建立了高效液相色谱-电感耦合等离子体质谱联用技术（HPLC-ICP-MS）测定大米粉中硒代胱氨酸（SeCys2）、甲基-硒代半胱氨酸（MeSeCys）、亚硒酸根（Se（Ⅳ））、硒代蛋氨酸（SeMet）、硒酸根（Se（Ⅵ））等5种硒形态的分析方法.采用Hamilton PRP-X100分析柱，以pH 4和pH 6的20 mmol·L-1的柠檬酸溶液辅以少量甲醇为流动相，在梯度洗脱条件下，9 min内可实现5种硒形态完全分离.SeCys2、MeSeCys、Se（Ⅳ）、SeMet、Se（Ⅵ）的检出限分别为0.88、0.69、1.00、0.90、0.82 μg·L-1.利用富硒小麦粉标准物质RM8436探讨了不同提取方式的提取效果.最终确定75 mmol·L-1 Tris-HCl（pH 7.5）添加XIV蛋白酶和Driselase酶作为提取剂，在37℃振荡提取3 h为大米样品前处理方法.利用该方法分析了富硒地区大米粉中的硒形态，结果表明，大米粉中的硒主要以有机硒形态为主，其中SeMet是最主要的硒形态，占总硒的90%左右.
    Abstract: An analytical method for the determination of five selenium species in rice flour samples was developed using high performance liquid chromatography combined with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Seleno-Cystine(SeCys2), (Methyl)Seleno-Cysteine (MeSeCys), Selenite(Se(Ⅳ)), Selenoate(Se(Ⅵ)) and Selenomethionine (SeMet) were completely separated on a Hamilton PRP-X100 anion exchange column using 20 mmol·L-1 citric acid solution (pH 4.0), 20 mmol·L-1 citric acid solution (pH 6.0) and methyl alcohol as the mobile phase under gradient elution within 9 minutes. The detection limits of SeCys2, MeSeCys, Se(Ⅳ), SeMet and Se(Ⅵ) were 0.88, 0.69, 1.00, 0.90, 0.82 μg·L-1, respectively. 75 mmol·L-1 Tris-HCl (pH 7.5) with XIV protease and Driselase was eventually selected as extractant. Different sample preparation methods had been compared and oscillation method with 3 h at 37 ℃ was chosen finally. This method was applied to measure selenium species in rice flour samples from selenium-enriched areas. The results indicated that SeMet was the main species in rice flour, accounting for about 90% of the total amount of selenium.
  • 加载中
  • [1] MATOS R P, LIMA V M P, WINDMOLLER C C, et al. Correlation between the natural levels of selenium and soil physicochemical characteristics from the Jequitinhonha Valley (MG), Brazil[J]. J Geochem Explor, 2017, 172:195-202.
    [2] BAKıRDERE S, VOLKAN M, ATAMAN O Y. Selenium speciation in chicken breast samples from inorganic and organic selenium fed chickens using high performance liquid chromatography-inductively coupled plasma-mass spectrometry[J]. Journal of Food Composition and Analysis, 2018, 71:28-35.
    [3] 董亚蕾,刘文婧,曹进,等. 超高效液相色谱-串联三重四极杆质谱法测定富硒食品中的硒代氨基酸[J]. 食品安全质量检测学报,2017,8(7):2401-2406.

    DONG Y L, LIU W J, CAO J, et al. Determination of selenoaminoacid in selenium enriched foods by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Journal of Food Safety and Quality 2017, 8(7):2401-2406(in Chinese).

    [4] 汪敏,庄海铃. 关于人体补硒标准的研究[J]. 数理医药学杂志,2007,20(4):549-550.

    WANG M, ZHUANG H L. Research on human body selenium supplementation standards[J]. Journal of Mathematical Medicine, 2007, 20(4):549-550(in Chinese).

    [5] PYRZYNSKA K. Determination of selenium species in environmental samples[J]. Microchimica Acta, 2002, 140(1-2):55-62.
    [6] MISRA S, BOYLAN M, SELVAM A, et al. Redox-active selenium compounds——from toxicity and cell death to cancer treatment[J]. Nutrients, 2015, 7(5):3536-3556.
    [7] HAWRYLAK-NOWAK B, MATRASZEK R, POGORZELEC M. The dual effects of two inorganic selenium forms on the growth, selected physiological parameters and macronutrients accumulation in cucumber plants[J]. Acta Physiol Plant, 2015, 37(2):41-54.
    [8] YIN H, QI Z, LI M, et al. Selenium forms and methods of application differentially modulate plant growth, photosynthesis, stress tolerance, selenium content and speciation in Oryza sativa L[J]. Ecotoxicology and Environmental Safety, 2019, 169:911-917.
    [9] 邵秀清,秦文友,张梦,等. 高效液相色谱串联质谱法检测蚯蚓体内硒代氨基酸[J]. 环境化学,2019,38(5):1057-1064.

    SHAO X Q, QIN W Y, ZHANG M, et al. Determination of seleno amino acids in earthworms by high performance liquid chromatography-electrospray ionization-mass spectrometry[J]. Environmental Chemistry, 2019, 38(5):1057-1064(in Chinese).

    [10] ZHOU F, DINH Q T, YANG W, et al. Assessment of speciation and in vitro bioaccessibility of selenium in Se-enriched Pleurotus ostreatus and potential health risks[J]. Ecotoxicol Environ Saf, 2019, 185:109675-109684.
    [11] 王欣,幸苑娜,陈泽勇,等. 高效液相色谱-电感耦合等离子体质谱法检测富硒食品中6种硒形态[J]. 分析化学,2013,41(11):1669-1674.

    WANG X, XING Y N, CHEN Z Y, et al. Determination of 6 selenium species in selenium-enriched food by hyphenated technique of high performance liquid chromatography inductively coupled plasma mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2013, 41(11):1669-1674(in Chinese).

    [12] 王铁良,张会芳,魏亮亮,等. 高效液相色谱-氢化物发生-原子荧光光谱联用技术测定富硒大米中的5种硒形态[J]. 食品安全质量检测学报,2017,8(6):2185-2190.

    WANG T L, ZHANG H F, WEI L L, et al. Determination of 5 kinds of selenium species in rice by high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry[J]. Journal of Food Safety and Quality 2017, 8(6):2185-2190(in Chinese).

    [13] 季海冰,方敏,林洋,等. 反相离子对色谱联合电感耦合等离子体质谱法测定环境水样中5种形态硒[J]. 分析仪器,2019,223(2):156-160.

    JI H B, FANG M, LIN Y, et al. Determination of five selenium species in environmental water by ionpair reversed phase liquid chroma tography with inductively coupled plasma mass spectrometry[J]. Analytical Instrumentation, 2019, 223(2):156-160(in Chinese).

    [14] 杨培慧,郑志雯,冯德雄,等. 硒代胱氨酸和硒代蛋氨酸电化学检测的比较[J]. 分析化学,2003,31(2):194-197.

    YANG P H, ZHENG Z W, FENG D X, et al. A comparison of electrochemical behavior between selenocystine and selenomethionine with cyclic voltammetry[J]. Chinese Journal of Analytical Chemistry, 2003, 31(2):194-197(in Chinese).

    [15] 倪张林,汤富彬,张玮,等. HPLC-DRC-ICP-MS测定富硒蔬菜中的硒形态[J]. 分析试验室,2013,32(2):39-43.

    YI Z L, TANG F B, ZHANG W, et al. Determination of selenium species in Se-enriched vegetables by HPLC-DRC-ICP-MS[J]. Chinese Journal of Analysis Laboratory 2012, 32(2):39-43(in Chinese).

    [16] 姚真真,马智宏,王北洪,等. 高效液相色谱-电感耦合等离子体质谱法检测富硒苹果中5种硒形态[J]. 食品安全质量检测学报,2018,9(3):475-480.

    YAO Z Z, HA X J, MA Z H, et al. Determination of 5 kinds of selenium species in selenium-enriched apples by high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Journal of Food Safety and Quality 2018, 9(3):475-480(in Chinese).

    [17] 曹玉嫔,闫丽珍,黄红丽,等. 超声辅助提取结合高效液相色谱-电感耦合等离子体质谱联用技术测定牛蒡和三七中硒形态[J]. 分析化学,2015,43(9):1329-1334.

    CAO Y P, YAN L Z, HUANG H L, et al. Determination of selenium species in burdock and panax notoginseng using ultrasonic assistant extraction combined with high performance liquid chromatographyinductively coupled plasma mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2015, 43(9):1329-1334(in Chinese).

    [18] 秦玉燕,时鹏涛,王运儒,等. 高效液相色谱-氢化物发生-原子荧光光谱法测定富硒食品中5种形态硒的含量[J]. 理化检测-化学分册,2018,54(5):566-572.

    QIN Y T, SHI P T, WANG Y, et al. HPLC-HG-AFS determination of 5 species of selenium in foodstuffs rich in selenium[J]. PTCA (Part B:Chem Anal), 2018, 54(5):556-572(in Chinese).

    [19] GOSETTI F, FRASCAROLO P, POLATI S, et al. Speciation of selenium in diet supplements by HPLC-MS/MS methods[J]. Food Chemistry, 2007, 105(4):1738-1747.
    [20] 张明,杜业刚,林少彬,等. 液相色谱串联质谱法定量检测青花菜中L-硒甲基硒代半胱氨酸[J]. 分析化学,2007,35(2):244-264.

    ZHANG M, DU Y G, LIN S B. Quantitaion of methylselenocysteine in broccoli powder with liquid chromatography-tandem mass spectrometry[J]. Analytical Chemistry, 2007, 35(2):244-264(in Chinese).

    [21] THOSAIKHAM W, JITMANEE K, SITTIPOUT R, et al. Evaluation of selenium species in selenium-enriched pakchoi (Brassica chinensis Jusl var parachinensis (Bailey) Tsen & Lee) using mixed ion-pair reversed phase HPLC-ICP-MS[J]. Food Chem, 2014, 145:736-742.
    [22]
    [23] ZHENG J, OHATA M, FURUTA N, et al. Speciation of selenium compounds with ion-pair reversed-phase liquid chromatography using inductively coupled plasma mass spectrometry as element-specific detection[J]. Journal of Chromatography A, 2000, 874(1):55-64.
    [24] JAGTAP R, MAHER W. Determination of selenium species in biota with an emphasis on animal tissues by HPLC-ICP-MS[J]. Microchemical Journal, 2016, 124:422-529.
    [25] TIE M, LI B, LIU Y, et al. HPLC-ICP-MS analysis of selenium speciation in selenium-enriched Cordyceps militaris[J]. RSC Adv, 2014, 4(107):62071-62075.
    [26] FUNES-COLLADO V, MORELL-GARCIA A, RUBIO R, et al. Selenium uptake by edible plants from enriched peat[J]. Scientia Horticulturae, 2013, 164(164):428-433.
    [27] GALINHA C, SANCHEZ-MARTINEZ M, PACHECO A M, et al. Characterization of selenium-enriched wheat by agronomic biofortification[J]. Food Sci Technol, 2015, 52(7):4236-4245.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  2178
  • HTML全文浏览数:  2178
  • PDF下载数:  87
  • 施引文献:  0
出版历程
  • 收稿日期:  2019-08-15
邵鹏威, 路国慧, 郑宇, 胡俊栋, 赵全升, 杨永亮, 刘崴. 高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态[J]. 环境化学, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
引用本文: 邵鹏威, 路国慧, 郑宇, 胡俊栋, 赵全升, 杨永亮, 刘崴. 高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态[J]. 环境化学, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
shu
SHAO Pengwei, LU Guohui, ZHENG Yu, HU Jundong, ZHAO Quansheng, YANG Yongliang, LIU Wei. Determination of selenium species in rice flour using high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Environmental Chemistry, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
Citation: SHAO Pengwei, LU Guohui, ZHENG Yu, HU Jundong, ZHAO Quansheng, YANG Yongliang, LIU Wei. Determination of selenium species in rice flour using high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Environmental Chemistry, 2020, (5): 1434-1441. doi: 10.7524/j.issn.0254-6108.2019081504
shu

高效液相色谱-电感耦合等离子体质谱测定大米粉中的硒形态

    通讯作者: 刘崴, E-mail: hattieliu@126.com
  • 1. 青岛大学环境科学与工程学院, 青岛, 266071;
  • 2. 国家地质实验测试中心, 北京, 100037
  • 收稿日期:  2019-08-15
基金项目:

中国地质科学院基本科研业务费项目（YYWF201620）和中国地质大调查项目（DD20190589）资助.

摘要: 建立了高效液相色谱-电感耦合等离子体质谱联用技术（HPLC-ICP-MS）测定大米粉中硒代胱氨酸（SeCys2）、甲基-硒代半胱氨酸（MeSeCys）、亚硒酸根（Se（Ⅳ））、硒代蛋氨酸（SeMet）、硒酸根（Se（Ⅵ））等5种硒形态的分析方法.采用Hamilton PRP-X100分析柱，以pH 4和pH 6的20 mmol·L-1的柠檬酸溶液辅以少量甲醇为流动相，在梯度洗脱条件下，9 min内可实现5种硒形态完全分离.SeCys2、MeSeCys、Se（Ⅳ）、SeMet、Se（Ⅵ）的检出限分别为0.88、0.69、1.00、0.90、0.82 μg·L-1.利用富硒小麦粉标准物质RM8436探讨了不同提取方式的提取效果.最终确定75 mmol·L-1 Tris-HCl（pH 7.5）添加XIV蛋白酶和Driselase酶作为提取剂，在37℃振荡提取3 h为大米样品前处理方法.利用该方法分析了富硒地区大米粉中的硒形态，结果表明，大米粉中的硒主要以有机硒形态为主，其中SeMet是最主要的硒形态，占总硒的90%左右.

English Abstract

    全文HTML

参考文献 (27)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心