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微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响

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顾艳芳, 邓媛, 梁婵娟. 微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响[J]. 环境化学, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
引用本文: 顾艳芳, 邓媛, 梁婵娟. 微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响[J]. 环境化学, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
shu
GU Yanfang, DENG Yuan, LIANG Chanjuan. Effect of microcystins on antioxidative enzymes activities and isozymes pattern in cucumber seedlings[J]. Environmental Chemistry, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
Citation: GU Yanfang, DENG Yuan, LIANG Chanjuan. Effect of microcystins on antioxidative enzymes activities and isozymes pattern in cucumber seedlings[J]. Environmental Chemistry, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
shu

微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响

  • 1. 江苏省厌氧生物技术重点实验室, 江南大学环境与土木工程学院, 无锡, 214122;

  • 2. 江苏省水处理技术与材料协同创新中心, 无锡, 214122

    • 通讯作者: 梁婵娟, E-mail: liangchanjuan@jiangnan.edu.cn
  • 基金项目:

    国家自然科学基金（31971407，31370517）和江苏省自然科学基金（BK20161131）资助.

Effect of microcystins on antioxidative enzymes activities and isozymes pattern in cucumber seedlings

  • 1. Jiangsu Province Key Laboratory of Anaerobic Biological Technology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, 214122, China;

  • 2. Jiangsu Cooperative Innovation Center of Technology and Material of Water Treatment, Wuxi, 214122, China

    • Corresponding author: LIANG Chanjuan, liangchanjuan@jiangnan.edu.cn
  • Fund Project: Supported by the National Natural Science Foundation of China (31971407, 31370517) and the Jiangsu Province Natural Science Foundation (BK20161131).

  • 摘要: 为了进一步清晰作物对微囊藻毒素（MCs）的适应性机制，本文通过水培实验研究了不同浓度MCs（5、10、50、100 μg·L-1）对黄瓜幼苗叶片抗氧化酶活性及其同工酶组成、活性氧（ROS）积累与质膜过氧化及生长的影响.结果表明，MCs（5—50 μg·L-1）胁迫7 d后黄瓜叶片中过氧化物酶（POD）和过氧化氢酶（CAT）同工酶条带变粗变亮，酶活性上升，其中POD活性增幅大于CAT，且POD同工酶有新条带出现（对照处理未见）.而超氧化物歧化酶（SOD）活性与其同工酶组成均无变化，因而超氧阴离子（O2·-）的增幅高于过氧化氢（H2O2），致使丙二醛（MDA）含量增加，而黄瓜幼苗干重降低.100 μg·L-1 MCs处理黄瓜叶片POD和CAT活性增幅最大，且同工酶条带宽度和亮度高于其他处理，但不同SOD同工酶出现不同的响应，SOD活性仍与对照无显著差异，O2·-增幅加大，导致氧化损伤程度加剧，黄瓜幼苗干重降幅增大.恢复7 d后，5 μg·L-1 MCs处理黄瓜叶片SOD活性、CAT活性、丙二醛含量和黄瓜幼苗干重恢复至对照水平，10 μg·L-1和50 μg·L-1 MCs处理黄瓜幼苗各指标均优于胁迫期，而100 μg·L-1 MCs处理黄瓜氧化损伤不可逆，生长难以恢复.综上，MCs胁迫下黄瓜幼苗CAT和POD同工酶表达量增加有利于增强酶活性，清除MCs诱发的H2O2积累，增强黄瓜对MCs的耐受性，且调控能力受MCs浓度限制.
    Abstract: To further clarify the adaptive mechanism of crops to microcystins (MCs), we studied effects of MCs (5, 10, 50 and 100 μg·L-1) on antioxidative enzymes activity, isozymes composition, reactive oxygen species (ROS) and plasma membrane peroxidation, and growth in cucumber (Cucumis sativus L.) seedlings by hydroponic experiments. Activities of peroxidase (POD) and catalase (CAT) were increased in cucumber leaves treated with 5, 10 or 50 μg·L-1 MCs for 7 d, and their isozyme bands were thicker and brighter. In addition, the increased degree of POD activity was greater than that of CAT as a new POD isoenzyme band appeared (not seen in the control treatment). However, the activity of superoxide dismutase (SOD) and its isoenzyme composition were not changed, and the increase in superoxide anion (O2·-) was higher than that of hydrogen peroxide (H2O2), which led malondialdehyde content increased and dry wight decreased. MCs at the concentration of 100 μg·L-1 caused the largest increase in activities of POD and CAT and the width and brightness of isozyme bands in leaves, but did not affect SOD activity. At the same treatment the increase in O2·- content aggravated oxidative damage and suppressed badly the accumulation of dry weight. After 7 d of recovery, activities of SOD and CAT, malondialdehyde content and dry weight in cucumber treated with 5 μg·L-1 MCs were all recovered to the control levels. All parameter above mentioned in cucumber seedlings treated with 10 or 50 μg·L-1 MCs were better than those measured during the stress period. However, the oxidative damage was irreversible and the growth was difficult to recover in cucumber seedlings treated with 100 μg·L-1 MCs. In conclusion, increase in CAT and POD isozymes expression in cucumber leaves caused by MCs stress were beneficial to increasing the enzyme activity and removing H2O2 induced by MCs, thus enhancing the tolerance of cucumber to MCs. In addition, the regulation ability of these antioxidative enzymes was limited by the concentration of MCs.
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  • 收稿日期:  2019-09-02
顾艳芳, 邓媛, 梁婵娟. 微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响[J]. 环境化学, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
引用本文: 顾艳芳, 邓媛, 梁婵娟. 微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响[J]. 环境化学, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
shu
GU Yanfang, DENG Yuan, LIANG Chanjuan. Effect of microcystins on antioxidative enzymes activities and isozymes pattern in cucumber seedlings[J]. Environmental Chemistry, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
Citation: GU Yanfang, DENG Yuan, LIANG Chanjuan. Effect of microcystins on antioxidative enzymes activities and isozymes pattern in cucumber seedlings[J]. Environmental Chemistry, 2020, (12): 3402-3409. doi: 10.7524/j.issn.0254-6108.2019090202
shu

微囊藻毒素对黄瓜幼苗抗氧化酶及其同工酶的影响

    通讯作者: 梁婵娟, E-mail: liangchanjuan@jiangnan.edu.cn
  • 1. 江苏省厌氧生物技术重点实验室, 江南大学环境与土木工程学院, 无锡, 214122;
  • 2. 江苏省水处理技术与材料协同创新中心, 无锡, 214122
  • 收稿日期:  2019-09-02
基金项目:

国家自然科学基金（31971407，31370517）和江苏省自然科学基金（BK20161131）资助.

摘要: 为了进一步清晰作物对微囊藻毒素（MCs）的适应性机制，本文通过水培实验研究了不同浓度MCs（5、10、50、100 μg·L-1）对黄瓜幼苗叶片抗氧化酶活性及其同工酶组成、活性氧（ROS）积累与质膜过氧化及生长的影响.结果表明，MCs（5—50 μg·L-1）胁迫7 d后黄瓜叶片中过氧化物酶（POD）和过氧化氢酶（CAT）同工酶条带变粗变亮，酶活性上升，其中POD活性增幅大于CAT，且POD同工酶有新条带出现（对照处理未见）.而超氧化物歧化酶（SOD）活性与其同工酶组成均无变化，因而超氧阴离子（O2·-）的增幅高于过氧化氢（H2O2），致使丙二醛（MDA）含量增加，而黄瓜幼苗干重降低.100 μg·L-1 MCs处理黄瓜叶片POD和CAT活性增幅最大，且同工酶条带宽度和亮度高于其他处理，但不同SOD同工酶出现不同的响应，SOD活性仍与对照无显著差异，O2·-增幅加大，导致氧化损伤程度加剧，黄瓜幼苗干重降幅增大.恢复7 d后，5 μg·L-1 MCs处理黄瓜叶片SOD活性、CAT活性、丙二醛含量和黄瓜幼苗干重恢复至对照水平，10 μg·L-1和50 μg·L-1 MCs处理黄瓜幼苗各指标均优于胁迫期，而100 μg·L-1 MCs处理黄瓜氧化损伤不可逆，生长难以恢复.综上，MCs胁迫下黄瓜幼苗CAT和POD同工酶表达量增加有利于增强酶活性，清除MCs诱发的H2O2积累，增强黄瓜对MCs的耐受性，且调控能力受MCs浓度限制.

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