稀有鮈鲫胚胎急性毒性试验的实验室间比对
Inter-laboratory Comparison for Fish Embryo Acute Toxicity Test with Chinese Rare Minnows
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摘要: 评价稀有鮈鲫胚胎急性毒性试验在各实验室的重现性，验证稀有鮈鲫胚胎是否适用于鱼类胚胎急性毒性试验，为相关化学品测试国家标准的制定提供依据。选择3，4-二氯苯胺和五水硫酸铜作为参比物质，按照《OECD化学品测试准则No.236鱼类胚胎急性毒性试验》，进行6家实验室间比对试验以评价试验结果的重现性。实验室间比对试验中，3，4-二氯苯胺和五水硫酸铜对稀有鮈鲫胚胎急性毒性96 h半数致死浓度（LC50）的总体平均值和标准差分别为（9.25±3.62） mg·L-1和（1.63±0.59） mg·L-1，变异系数分别为39.1%和36.0%。采用z分数方法评价各实验室间比对试验结果的差异大小，各实验室3，4-二氯苯胺和五水硫酸铜比对结果的z分数分别在-1.42~1.23之间和-0.898~1.56之间。虽然变异系数较大，但各实验室的96 h-LC50均在xpt±2σpt范围之内。稀有鮈鲫胚胎急性毒性试验结果具有良好的重现性，且稀有鮈鲫胚胎对3，4-二氯苯胺和五水硫酸铜的敏感性和重复性与斑马鱼（Danio rerio）类似，毒性值差异均未超过一个数量级，因此稀有鮈鲫可作为鱼类胚胎急性毒性试验的本土受试鱼种。
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关键词:
- 稀有鮈鲫 /
- 鱼类胚胎急性毒性试验 /
- 实验室间比对 /
- 重现性
Abstract: By comparing the results of Chinese rare minnows (Gobiocypris rarus) embryo acute toxicity test from different laboratories, this study aims to validate whether Gobiocypris rarus embryos are applicable for the fish embryo acute toxicity test as a test organism, and to lay a foundation for the national standards of chemical testing. According to "OECD Guidelines for the Testing of Chemicals No. 236:Fish Embryo Acute Toxicity (FET) Test", six domestic laboratories were involved in the ring test program to evaluate the reproducibility of the method. Gobiocypris rarus embryo were exposed to two reference substances (3,4-dichloroaniline and copper sulfate pentahydrate), and the overall means of the concentration that is estimated to be lethal to 50% of embryos after 96 h exposure relative to controls (LC50) were (9.25±3.62) mg·L-1 for 3,4-dichloroaniline and (1.63±0.59) mg·L-1 for copper sulfate pentahydrate, with coefficient of variation (CV) of 39.1% and 36.0%, respectively. The difference in the results of the inter-laboratory testing were evaluated by z-score method, and a range from -1.42 to 1.23 for 3,4-dichloroaniline and that from -0.898 to 1.56 for copper sulfate pentahydrate were obtained. Although the coefficients of variation of both datasets were significant, the 96 h-LC50 of the six laboratories were within the range of xpt±2σpt. The reproducibility of this testing method using Gobiocypris rarus embryos has presented a promising result, and the level of sensitivity and repeatability of this native fish species embryo is comparable to that of Danio rerio embryo in relation to the exposure of 3,4-dichloroaniline and copper sulfate pentahydrate, i.e., the differences of the two species less than an order of magnitude. Therefore, Gobiocypris rarus can serve as standard test organism for fish toxicity studies. -
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