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醚菌酯对斑马鱼早期生命阶段的慢性毒性效应

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胡浩泽, 王卢燕, 李烨青, 张昌朋, 赵学平, 张鑫, 蒋金花. 醚菌酯对斑马鱼早期生命阶段的慢性毒性效应[J]. 生态毒理学报, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
引用本文: 胡浩泽, 王卢燕, 李烨青, 张昌朋, 赵学平, 张鑫, 蒋金花. 醚菌酯对斑马鱼早期生命阶段的慢性毒性效应[J]. 生态毒理学报, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
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Hu Haoze, Wang Luyan, Li Yeqing, Zhang Changpeng, Zhao Xueping, Zhang Xin, Jiang Jinhua. Chronic Toxic Effects of Kresoxim-methyl during Zebrafish Early Life Stage[J]. Asian journal of ecotoxicology, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
Citation: Hu Haoze, Wang Luyan, Li Yeqing, Zhang Changpeng, Zhao Xueping, Zhang Xin, Jiang Jinhua. Chronic Toxic Effects of Kresoxim-methyl during Zebrafish Early Life Stage[J]. Asian journal of ecotoxicology, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
shu

醚菌酯对斑马鱼早期生命阶段的慢性毒性效应

  • 1. 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 宁波大学食品与药学学院, 宁波 315800;

  • 2. 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 浙江省农业科学院农产品质量安全与营养研究所, 杭州 310021

    • 作者简介: 胡浩泽(1998-),男,硕士研究生,研究方向为农药环境安全评估,E-mail:huhaoze0130@163.com
    通讯作者: 蒋金花,E-mail: jiangjh@zaas.ac.cn
  • 基金项目:

    国家自然科学基金面上项目(32072455)

  • 中图分类号: X171.5

Chronic Toxic Effects of Kresoxim-methyl during Zebrafish Early Life Stage

  • 1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, College of Food and Pharmacy, Ningbo University, Ningbo 315800, China;

  • 2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

    • Corresponding author: Jiang Jinhua, jiangjh@zaas.ac.cn
  • Fund Project:

  • 摘要: 为探明甲氧基丙烯酸酯类杀菌剂(strobilurins)对水生生物早期生命阶段的慢性毒性，以斑马鱼为模式生物，测定了醚菌酯长期暴露对斑马鱼早期发育的亚致死效应。将斑马鱼胚胎持续暴露于不同浓度醚菌酯(0.000750～0.180 mg·L-1)34 d后，醚菌酯对斑马鱼早期生命阶段的144 h和34 d半致死浓度(LC50)分别为0.141 mg·L-1和0.0106 mg·L-1。研究发现，醚菌酯暴露34 d后，0.00460、0.0115和0.0288 mg·L-1醚菌酯可显著降低仔鱼的体质量，0.0115 mg·L-1和0.0288 mg·L-1醚菌酯能够降低仔鱼体长，而0.180 mg·L-1醚菌酯会增加仔鱼的体长和体质量。醚菌酯对斑马鱼早期生命阶段体长的34 d-NOEC为0.00460 mg·L-1，34 d-LOEC为0.0115 mg·L-1，对早期生命阶段体质量的34 d-NOEC为0.00185 mg·L-1，34 d-LOEC为0.00460 mg·L-1。在试验期间，分别于1、3、7、14、21和34 d测定斑马鱼胚胎/仔鱼体内与线粒体能量代谢和氧化应激相关因子的活性和水平。研究发现，醚菌酯长期暴露会导致斑马鱼早期生命阶段过氧化氢酶(CAT)活性升高，过氧化物酶(POD)活性先升高后降低再升高，超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量先降低再升高。此外，醚菌酯长期暴露期间，斑马鱼体内钙离子(Ca2+)水平和钙离子腺苷三磷酸酶(Ca2+-ATP酶)活性受到不同程度的抑制，三磷酸腺苷(ATP)水平先升高后降低。研究表明，醚菌酯长期暴露会对斑马鱼早期生命阶段的生长指标和抗氧化系统产生一定的负面影响，醚菌酯可能通过调控ATP和Ca2+水平、Ca2+-ATP酶活性，干扰线粒体能量代谢进而诱导斑马鱼早期生命阶段产生氧化应激效应。
    Abstract: In order to investigate the chronic toxicity of strobilurins to the early life stage of aquatic organisms, the sublethal effect of kresoxim-methyl on the early development of zebrafish after long-term exposure was determined. After continuous exposure of zebrafish embryos to different concentrations of kresoxim-methyl (0.000750～0.180 mg·L-1) for 34 d, the median lethal concentrations (LC50) of kresoxim-methyl for 144 h and 34 d of zebrafish early life stage was 0.141 mg·L-1 and 0.0106 mg·L-1, respectively. It was found that 0.00460 mg·L-1, 0.0115 mg·L-1 and 0.0288 mg·L-1 kresoxim-methyl significantly decreased the body weight of larvae after 34 d exposure, 0.0115 mg·L-1 and 0.0288 mg·L-1 kresoxim-methyl decreased the body length of larvae, and 0.180 mg·L-1 kresoxim-methyl increased the body length and body weight of larvae. The 34 d-NOEC and 34 d-LOEC of kresoxim-methyl was 0.00460 mg·L-1 and 0.0115 mg·L-1 for body length, and was 0.00185 mg·L-1 and 0.00460 mg·L-1 for body weight during zebrafish early life development, respectively. During the 34 d exposure, the activities and levels of factors related to mitochondrial energy metabolism and oxidative stress in zebrafish embryos/larvae were measured at 1, 3, 7, 14, 21 and 34 d. Results showed that long-term exposure of kresoxim-methyl could increase the catalase (CAT) activity, the peroxide (POD) activity increased then decreased and then increased, the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content decreased first then increased during zebrafish early life development. It was found that the calcium ion (Ca2+) level and the activity of calcium ion adenosine triphosphatase (Ca2+-ATPase) was inhibited during the 34 d exposure of kresoxim-methyl, and the adenosine triphosphate (ATP) level was increased first then decreased. The results suggested long-term exposure to kresoxim-methyl caused negative effects on the growth indexes and antioxidant system during zebrafish early life development, kresoxim-methyl might interfere with mitochondrial energy metabolism by regulating the ATP and Ca2+ levels, and Ca2+-ATPase activity, then induced oxidative stress during the early life stage of zebrafish.
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  • 收稿日期:  2022-11-03
胡浩泽, 王卢燕, 李烨青, 张昌朋, 赵学平, 张鑫, 蒋金花. 醚菌酯对斑马鱼早期生命阶段的慢性毒性效应[J]. 生态毒理学报, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
引用本文: 胡浩泽, 王卢燕, 李烨青, 张昌朋, 赵学平, 张鑫, 蒋金花. 醚菌酯对斑马鱼早期生命阶段的慢性毒性效应[J]. 生态毒理学报, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
shu
Hu Haoze, Wang Luyan, Li Yeqing, Zhang Changpeng, Zhao Xueping, Zhang Xin, Jiang Jinhua. Chronic Toxic Effects of Kresoxim-methyl during Zebrafish Early Life Stage[J]. Asian journal of ecotoxicology, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
Citation: Hu Haoze, Wang Luyan, Li Yeqing, Zhang Changpeng, Zhao Xueping, Zhang Xin, Jiang Jinhua. Chronic Toxic Effects of Kresoxim-methyl during Zebrafish Early Life Stage[J]. Asian journal of ecotoxicology, 2024, 19(1): 275-285. doi: 10.7524/AJE.1673-5897.20221103001
shu

醚菌酯对斑马鱼早期生命阶段的慢性毒性效应

    通讯作者: 蒋金花,E-mail: jiangjh@zaas.ac.cn
    作者简介: 胡浩泽(1998-),男,硕士研究生,研究方向为农药环境安全评估,E-mail:huhaoze0130@163.com
  • 1. 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 宁波大学食品与药学学院, 宁波 315800;
  • 2. 省部共建农产品质量安全危害因子与风险防控国家重点实验室, 浙江省农业科学院农产品质量安全与营养研究所, 杭州 310021
  • 收稿日期:  2022-11-03
基金项目:

国家自然科学基金面上项目(32072455)

摘要: 为探明甲氧基丙烯酸酯类杀菌剂(strobilurins)对水生生物早期生命阶段的慢性毒性，以斑马鱼为模式生物，测定了醚菌酯长期暴露对斑马鱼早期发育的亚致死效应。将斑马鱼胚胎持续暴露于不同浓度醚菌酯(0.000750～0.180 mg·L-1)34 d后，醚菌酯对斑马鱼早期生命阶段的144 h和34 d半致死浓度(LC50)分别为0.141 mg·L-1和0.0106 mg·L-1。研究发现，醚菌酯暴露34 d后，0.00460、0.0115和0.0288 mg·L-1醚菌酯可显著降低仔鱼的体质量，0.0115 mg·L-1和0.0288 mg·L-1醚菌酯能够降低仔鱼体长，而0.180 mg·L-1醚菌酯会增加仔鱼的体长和体质量。醚菌酯对斑马鱼早期生命阶段体长的34 d-NOEC为0.00460 mg·L-1，34 d-LOEC为0.0115 mg·L-1，对早期生命阶段体质量的34 d-NOEC为0.00185 mg·L-1，34 d-LOEC为0.00460 mg·L-1。在试验期间，分别于1、3、7、14、21和34 d测定斑马鱼胚胎/仔鱼体内与线粒体能量代谢和氧化应激相关因子的活性和水平。研究发现，醚菌酯长期暴露会导致斑马鱼早期生命阶段过氧化氢酶(CAT)活性升高，过氧化物酶(POD)活性先升高后降低再升高，超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量先降低再升高。此外，醚菌酯长期暴露期间，斑马鱼体内钙离子(Ca2+)水平和钙离子腺苷三磷酸酶(Ca2+-ATP酶)活性受到不同程度的抑制，三磷酸腺苷(ATP)水平先升高后降低。研究表明，醚菌酯长期暴露会对斑马鱼早期生命阶段的生长指标和抗氧化系统产生一定的负面影响，醚菌酯可能通过调控ATP和Ca2+水平、Ca2+-ATP酶活性，干扰线粒体能量代谢进而诱导斑马鱼早期生命阶段产生氧化应激效应。

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