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弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原

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刘宝敏, 袁东星, 吴翠琴. 弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原[J]. 环境化学, 2006, 25(3): 306-309.
引用本文: 刘宝敏, 袁东星, 吴翠琴. 弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原[J]. 环境化学, 2006, 25(3): 306-309.
shu
LIU Bao-min, YUAN Dong-xing, WU Cui-qin. PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION[J]. Environmental Chemistry, 2006, 25(3): 306-309.
Citation: LIU Bao-min, YUAN Dong-xing, WU Cui-qin. PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION[J]. Environmental Chemistry, 2006, 25(3): 306-309.
shu

弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原

  • 1.

    近海海洋环境科学国家重点实验室, 厦门大学环境科学研究中心, 厦门, 361005

  • 基金项目:

    国家自然科学基金(20277030)资助项目

PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION

  • 1.

    State Key Laboratory of Marine Environmental ScienceEnvironmental Science Research Center, Xiamen University, Xiamen, 361005, China

  • Fund Project:

  • 摘要: 利用弱阴离子交换色谱,从雌激素17β-雌二醇诱导的真鲷鱼血清中分离纯化出卵黄蛋白原粗组分,以超滤法进一步除去杂质蛋白,并脱盐浓缩,获得纯度较高的卵黄蛋白原(Vtg),两级纯化得到的Vtg经常规聚丙烯酰胺凝胶电泳(Native-PAGE)分析,证实真鲷鱼Vtg有三种形式,分子量分别为665kDa,635kDa和355kDa;变性聚丙烯酰胺凝胶电泳(SDS-PAGE)分析结果表明,它们由185kDa左右的蛋白亚基组成.
    Abstract: With ultrafiltration and anion exchange liquid chromatography,a two-step method had been developed for purification of vitellogenin(Vtg) from the serum of Pagromus major induced by 17β-estradiol(E2).The(results) showed that most impurities could be removed during the first step by weak anion exchange liquid(chromatography) and further clean-up through ultrafiltration.In Native-PAGE,the Vtg purified by the two steps showed three forms,with molecular weights approximately 665kDa,635kDa and 355kDa,respectively.The result of SDS-PAGE indicated that the molecular weight of Vtg monomers was about 185kDa.High resolution anion exchange high performance liquid chromatography was applied to analyze the Vtg purified from the two-step method,and approved the high purity.The purified Vtg could be used for further analysis.
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刘宝敏, 袁东星, 吴翠琴. 弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原[J]. 环境化学, 2006, 25(3): 306-309.
引用本文: 刘宝敏, 袁东星, 吴翠琴. 弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原[J]. 环境化学, 2006, 25(3): 306-309.
shu
LIU Bao-min, YUAN Dong-xing, WU Cui-qin. PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION[J]. Environmental Chemistry, 2006, 25(3): 306-309.
Citation: LIU Bao-min, YUAN Dong-xing, WU Cui-qin. PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION[J]. Environmental Chemistry, 2006, 25(3): 306-309.
shu

弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原

  • 1. 近海海洋环境科学国家重点实验室, 厦门大学环境科学研究中心, 厦门, 361005
基金项目:

国家自然科学基金(20277030)资助项目

摘要: 利用弱阴离子交换色谱,从雌激素17β-雌二醇诱导的真鲷鱼血清中分离纯化出卵黄蛋白原粗组分,以超滤法进一步除去杂质蛋白,并脱盐浓缩,获得纯度较高的卵黄蛋白原(Vtg),两级纯化得到的Vtg经常规聚丙烯酰胺凝胶电泳(Native-PAGE)分析,证实真鲷鱼Vtg有三种形式,分子量分别为665kDa,635kDa和355kDa;变性聚丙烯酰胺凝胶电泳(SDS-PAGE)分析结果表明,它们由185kDa左右的蛋白亚基组成.

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