弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原

刘宝敏; 袁东星; 吴翠琴

近海海洋环境科学国家重点实验室, 厦门大学环境科学研究中心, 厦门, 361005

基金项目:

国家自然科学基金(20277030)资助项目

PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION

State Key Laboratory of Marine Environmental ScienceEnvironmental Science Research Center, Xiamen University, Xiamen, 361005, China

Fund Project:

摘要: 利用弱阴离子交换色谱,从雌激素17β-雌二醇诱导的真鲷鱼血清中分离纯化出卵黄蛋白原粗组分,以超滤法进一步除去杂质蛋白,并脱盐浓缩,获得纯度较高的卵黄蛋白原(Vtg),两级纯化得到的Vtg经常规聚丙烯酰胺凝胶电泳(Native-PAGE)分析,证实真鲷鱼Vtg有三种形式,分子量分别为665kDa,635kDa和355kDa;变性聚丙烯酰胺凝胶电泳(SDS-PAGE)分析结果表明,它们由185kDa左右的蛋白亚基组成.

Abstract: With ultrafiltration and anion exchange liquid chromatography,a two-step method had been developed for purification of vitellogenin(Vtg) from the serum of Pagromus major induced by 17β-estradiol(E2).The(results) showed that most impurities could be removed during the first step by weak anion exchange liquid(chromatography) and further clean-up through ultrafiltration.In Native-PAGE,the Vtg purified by the two steps showed three forms,with molecular weights approximately 665kDa,635kDa and 355kDa,respectively.The result of SDS-PAGE indicated that the molecular weight of Vtg monomers was about 185kDa.High resolution anion exchange high performance liquid chromatography was applied to analyze the Vtg purified from the two-step method,and approved the high purity.The purified Vtg could be used for further analysis.

Key words:

weak anion exchange liquid chromatography /
ultrafiltration /
vitellogenin

弱阴离子交换-超滤两级纯化浓缩真鲷卵黄蛋白原

1. 近海海洋环境科学国家重点实验室, 厦门大学环境科学研究中心, 厦门, 361005

基金项目:

国家自然科学基金(20277030)资助项目

关键词:

PURIFICATION AND CONCENTRATION OF VITELLOGENIN FROM PAGROSOMUS BY ANION EXCHANGE LIQUID CHROMATOGRAPHY AND ULTRAFILTRATION

1. State Key Laboratory of Marine Environmental ScienceEnvironmental Science Research Center, Xiamen University, Xiamen, 361005, China

Fund Project:

Keywords:

weak anion exchange liquid chromatography /
ultrafiltration /
vitellogenin

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