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低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究

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白琦锋, 高礼, 袁涛. 低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究[J]. 环境化学, 2012, 31(5): 720-725.
引用本文: 白琦锋, 高礼, 袁涛. 低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究[J]. 环境化学, 2012, 31(5): 720-725.
shu
BAI Qifeng, GAO Li, YUAN Tao. Toxicity of low concentration exposures of triclosan and triclocarban on Tetrahymena thermophila[J]. Environmental Chemistry, 2012, 31(5): 720-725.
Citation: BAI Qifeng, GAO Li, YUAN Tao. Toxicity of low concentration exposures of triclosan and triclocarban on Tetrahymena thermophila[J]. Environmental Chemistry, 2012, 31(5): 720-725.
shu

低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究

  • 1.

    上海交通大学环境科学与工程学院, 上海, 200240;

  • 2.

    宁夏大学资源与环境学院, 银川, 750021

  • 基金项目:

    上海科委基础研究重点项目(10JC1407800)资助.

Toxicity of low concentration exposures of triclosan and triclocarban on Tetrahymena thermophila

  • 1.

    School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai, 200240, China;

  • 2.

    School of Resource and Environment, Ningxia University, Yinchuan, 750021, China

  • Fund Project:

  • 摘要: 研究了环境相关浓度水平(μg·L-1)的三氯生(TCS)和三氯卡班(TCC)暴露对嗜热四膜虫的生长抑制效应,并尝试探索这两种新生污染物对四膜虫细胞活性的影响.结果表明,μg·L-1水平的TCS和TCC对四膜虫的生长存在明显抑制作用,24 h-EC50分别为141 μg·L-1、728 μg·L-1;最低无效应浓度(NOEC)分别为2 μg·L-1、30 μg·L-1;最低效应浓度(LOEC)分别为4 μg·L-1、61 μg·L-1.其中,TCC在1—10 μg·L-1水平表现出促进作用,可能是hormesis效应的体现.另外,在TCS和TCC浓度达到1000 μg·L-1时均对四膜虫细胞膜产生明显损伤;TCS和TCC也影响其溶酶体活性,1 μg·L-1暴露2 h时,荧光值(RFU)百分比分别降低至对照样品的88.62%、95.75%.本研究结果有助于进一步从亚细胞和分子水平认识环境污染水平TCS和TCC的生态毒性,也为这两种新生污染物在环境中的生态风险评价提供重要参考依据.
    Abstract: Triclosan (TCS) and triclocarban (TCC),as two typical antibacterial agents, have been widely used in many kinds of cleaning products. Their ecotoxicity has become increasing concern, as their occurrences in environmental matrix have been frequently reported. In this paper, we studied the growth inhibition effects of environmentally relevant concentration exposure of TCS and TCC on Tetrahymena thermophila. The changes of the cell viability of Tetrahymena were further investigated. The results showed that the growth of Tetrahymena was inhibited significantly with μg·L-1 level TCS and TCC. The 24 h-EC50 of TCS and TCC was 141 μg·L-1and 728 μg·L-1, with NOEC of 2 μg·L-1 and 30 μg·L-1, LOEC of 4 μg·L-1 and 61 μg·L-1,respectively. Particularly,TCC seemly presented hormesis effect as it had growth stimulation at 1 to10 μg·L-1. In addition, when the concentrations of TCS and TCC reached 1000 μg·L-1, obvious damages of cell membrane were observed. Also, the activity of lysosomes was reduced to 88.62% and 95.75% of the control fluorescence values, respectively, when the cells were exposed to 1 μg·L-1of TCS and TCC for 2 h. This study provides important references for the ecological risk assessments of these two emerging pollutants.
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  • 收稿日期:  2011-09-21
白琦锋, 高礼, 袁涛. 低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究[J]. 环境化学, 2012, 31(5): 720-725.
引用本文: 白琦锋, 高礼, 袁涛. 低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究[J]. 环境化学, 2012, 31(5): 720-725.
shu
BAI Qifeng, GAO Li, YUAN Tao. Toxicity of low concentration exposures of triclosan and triclocarban on Tetrahymena thermophila[J]. Environmental Chemistry, 2012, 31(5): 720-725.
Citation: BAI Qifeng, GAO Li, YUAN Tao. Toxicity of low concentration exposures of triclosan and triclocarban on Tetrahymena thermophila[J]. Environmental Chemistry, 2012, 31(5): 720-725.
shu

低剂量三氯生和三氯卡班对嗜热四膜虫的毒性研究

  • 1.  上海交通大学环境科学与工程学院, 上海, 200240;
  • 2.  宁夏大学资源与环境学院, 银川, 750021
  • 收稿日期:  2011-09-21
基金项目:

上海科委基础研究重点项目(10JC1407800)资助.

摘要: 研究了环境相关浓度水平(μg·L-1)的三氯生(TCS)和三氯卡班(TCC)暴露对嗜热四膜虫的生长抑制效应,并尝试探索这两种新生污染物对四膜虫细胞活性的影响.结果表明,μg·L-1水平的TCS和TCC对四膜虫的生长存在明显抑制作用,24 h-EC50分别为141 μg·L-1、728 μg·L-1;最低无效应浓度(NOEC)分别为2 μg·L-1、30 μg·L-1;最低效应浓度(LOEC)分别为4 μg·L-1、61 μg·L-1.其中,TCC在1—10 μg·L-1水平表现出促进作用,可能是hormesis效应的体现.另外,在TCS和TCC浓度达到1000 μg·L-1时均对四膜虫细胞膜产生明显损伤;TCS和TCC也影响其溶酶体活性,1 μg·L-1暴露2 h时,荧光值(RFU)百分比分别降低至对照样品的88.62%、95.75%.本研究结果有助于进一步从亚细胞和分子水平认识环境污染水平TCS和TCC的生态毒性,也为这两种新生污染物在环境中的生态风险评价提供重要参考依据.

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