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镉对小鼠骨生物力学及成骨细胞MG-63的影响

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黄泽昊, 侯艳丽, 李君平, 王立新, 张昌文, 王小岩, 荣海钦, 任建武, 曲广波. 镉对小鼠骨生物力学及成骨细胞MG-63的影响[J]. 环境化学, 2012, 31(12): 1835-1839.
引用本文: 黄泽昊, 侯艳丽, 李君平, 王立新, 张昌文, 王小岩, 荣海钦, 任建武, 曲广波. 镉对小鼠骨生物力学及成骨细胞MG-63的影响[J]. 环境化学, 2012, 31(12): 1835-1839.
shu
HUANG Zehao, HOU Yanli, LI Junping, WANG Lixin, ZHANG Changwen, WANG Xiaoyan, RONG Haiqin, REN Jianwu, QU Guangbo. Effects of cadmium on bone biomechanics in mice and osteoblast MG-63[J]. Environmental Chemistry, 2012, 31(12): 1835-1839.
Citation: HUANG Zehao, HOU Yanli, LI Junping, WANG Lixin, ZHANG Changwen, WANG Xiaoyan, RONG Haiqin, REN Jianwu, QU Guangbo. Effects of cadmium on bone biomechanics in mice and osteoblast MG-63[J]. Environmental Chemistry, 2012, 31(12): 1835-1839.
shu

镉对小鼠骨生物力学及成骨细胞MG-63的影响

  • 1.

    北京林业大学, 北京, 100083;

  • 2.

    中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室, 北京, 100085;

  • 3.

    山东省内分泌与代谢病研究所, 济南, 250062

  • 基金项目:

    国家自然基金(21077128, 20921063, 21177151)资助.

Effects of cadmium on bone biomechanics in mice and osteoblast MG-63

  • 1.

    Beijing Forestry University, Beijing, 100083, China;

  • 2.

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China;

  • 3.

    Shandong Institute of Endocrine and Metabolic Diseases, Jinan, 250062, China

  • Fund Project:

  • 摘要: 为了探讨镉对骨的影响及机制,选取7周龄C57BL/6J小鼠给予氯化镉2 mgkg-1腹腔注射,每周2次,染毒4周后进行了骨力学参数的测量.结果显示,暴露组股骨的最大弯曲应力及弹性模量降低,骨灰干重比下降.体外结果显示,氯化镉(CdCl2)的暴露影响人成骨肉瘤细胞MG-63的细胞形态、活力及增殖,并且呈剂量依赖性.因此,氯化镉暴露可导致小鼠骨量下降和骨力学性能下降,并对成骨细胞有明显的毒性.
    Abstract: Cadmium is one of the most widely distributed heavy metals in the environment. In this study, C57BL/6 J mice were injected intraperitoneally with cadmium chloride at 2 mg穔g-1 twice per week. After 4 week exposure, femur was taken out for 3-point-bending test and ashes-dry weight ratio evaluation. The maximal bending stress, elastic modulus and ashes-dry weight ratio of the femur were lower in the exposure group than that in the control one. Cadmium also affected the morphology, activity and proliferation of osteoblast-like cells MG-63 in a dose-dependent manner. Our results suggest that cadmium exposure induces the decrease in bone mass and biomechanics in mice, and obvious toxicity to osteoblasts.
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  • [1] 王崇臣, 黄忠臣, 王鹏. 北京四环公路两侧植物铅、镉污染现状调查[J]. 环境化学, 2009,28(4):604-605
    [2] [6] Kazantzis G. Cadmium,osteoporosis and calcium metabolism [J]. Biometals, 2004, 17(5):493-498
    [3] [7] Aitto A, Tritscher A. Effects on health of cadmium-WHO approaches and conclusions [J]. Biometals, 2004, 17(5):491-491
    [4] [8] Akesson A, Bjellerup P, Lundh T, et al. Cadmium-induced effects on bone in a population-based study of women [J]. Environ Health Persp, 2006, 114(6):830-834
    [5] [9] Alfvén T, Elinder C G, Carlsson M D, et al. Low-level cadmium exposure and osteoporosis [J]. Bone Miner Res, 2000, 15(8):1579-1586
    [6] [10] Alfvén T, Järup L, Elinder C G. Cadmium and lead in blood in relation to low bone mineral density and tubular proteinuria. [J]. Environ Health Persp, 2002, 110(7):699-702
    [7] [11] Ando M, Sayato Y, Osawa T. Studies on the disposition of calcium in bones of rats after continuous oral administration of cadmium [J]. Toxicol Appl Pharm, 1978, 46:625-632
    [8] [12] Brzóska M M, Moniuszko-Jakoniuk J. Low-level exposure to cadmium during the lifetime increases the risk of osteoporosis and fractures of the lumbar spine in the elderly: Studies on a rat model of human environmental exposure [J]. Toxicol Sci, 2004, 82(2):468-477
    [9] [13] Brzóska M M, Moniuszko-Jakoniuk J. Low-level lifetime exposure to cadmium decreases skeletal mineralization and enhances bone loss in aged rats [J]. Bone, 2004, 35(5):1180-1191
    [10] [14] Miyahara T, Tonoyama H, Watanabe M, et al. Stimulative effect of cadmium on prostaglandin E2 production in primary mouse osteoblastic cells [J]. Calcified Tissue Int, 2001, 68(3):185-191
    [11] [15] Comelekoglu U,Yalin S,Bagis S,et al. Low-exposure cadmium is more toxic on osteoporotic rat femoral bone: mechanical, biochemical, and histopathological evaluation [J]. Ecotox Environ Safe, 2007, 66(2):267-271
    [12] [16] Brzóska M M, Moniuszko-Jakoniuk J. Bone metabolism of male rats chronically exposed to cadmium [J]. Toxicol Appl Pharm, 2005, 207(3):195-211
    [13] [17] Lill CA, Hesseln J, Schlegel U, et al. Biomechanical evaluation of healing in a non-critical defect in a large animal model of osteoporosis [J]. J Orthop Res, 2003, 21(5):836-842
    [14] [19] Lévesque M, Martineau C, Jumarie C, et al. Characterization of cadmium uptake and cytotoxicity in human osteoblast-like MG-63 cells [J]. Toxicol Appl Pharm, 2008, 231(8):308-317
    [15] [20] Chen X, Zhu G, Gu S, et al. Effects of cadmium on osteoblasts and osteoclasts in vitro [J]. Environ Toxicol Pharm, 2009, 28(2):232-236
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  • 收稿日期:  2012-02-21
黄泽昊, 侯艳丽, 李君平, 王立新, 张昌文, 王小岩, 荣海钦, 任建武, 曲广波. 镉对小鼠骨生物力学及成骨细胞MG-63的影响[J]. 环境化学, 2012, 31(12): 1835-1839.
引用本文: 黄泽昊, 侯艳丽, 李君平, 王立新, 张昌文, 王小岩, 荣海钦, 任建武, 曲广波. 镉对小鼠骨生物力学及成骨细胞MG-63的影响[J]. 环境化学, 2012, 31(12): 1835-1839.
shu
HUANG Zehao, HOU Yanli, LI Junping, WANG Lixin, ZHANG Changwen, WANG Xiaoyan, RONG Haiqin, REN Jianwu, QU Guangbo. Effects of cadmium on bone biomechanics in mice and osteoblast MG-63[J]. Environmental Chemistry, 2012, 31(12): 1835-1839.
Citation: HUANG Zehao, HOU Yanli, LI Junping, WANG Lixin, ZHANG Changwen, WANG Xiaoyan, RONG Haiqin, REN Jianwu, QU Guangbo. Effects of cadmium on bone biomechanics in mice and osteoblast MG-63[J]. Environmental Chemistry, 2012, 31(12): 1835-1839.
shu

镉对小鼠骨生物力学及成骨细胞MG-63的影响

  • 1.  北京林业大学, 北京, 100083;
  • 2.  中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室, 北京, 100085;
  • 3.  山东省内分泌与代谢病研究所, 济南, 250062
  • 收稿日期:  2012-02-21
基金项目:

国家自然基金(21077128, 20921063, 21177151)资助.

摘要: 为了探讨镉对骨的影响及机制,选取7周龄C57BL/6J小鼠给予氯化镉2 mgkg-1腹腔注射,每周2次,染毒4周后进行了骨力学参数的测量.结果显示,暴露组股骨的最大弯曲应力及弹性模量降低,骨灰干重比下降.体外结果显示,氯化镉(CdCl2)的暴露影响人成骨肉瘤细胞MG-63的细胞形态、活力及增殖,并且呈剂量依赖性.因此,氯化镉暴露可导致小鼠骨量下降和骨力学性能下降,并对成骨细胞有明显的毒性.

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