Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制

徐芳芳; 黄满红; 陈亮; 柿井一男

环境化学

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徐芳芳, 黄满红, 陈亮, 柿井一男. Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制[J]. 环境化学, 2012, 31(12): 1840-1848.

引用本文:

徐芳芳, 黄满红, 陈亮, 柿井一男. Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制[J]. 环境化学, 2012, 31(12): 1840-1848.

XU Fangfang, HUANG Manhong, CHEN Liang, KAZUO Kakii. Toxicity and mechanism of Cu(Ⅱ),Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli under weak acidic conditions[J]. Environmental Chemistry, 2012, 31(12): 1840-1848.

Citation:

XU Fangfang, HUANG Manhong, CHEN Liang, KAZUO Kakii. Toxicity and mechanism of Cu(Ⅱ),Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli under weak acidic conditions[J]. Environmental Chemistry, 2012, 31(12): 1840-1848.

Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制

1.
东华大学环境科学与工程学院, 上海, 201620;
2.
日本宇都宫大学材料与环境化学系, 宇都宫, 321-0904
基金项目:
国家自然科学基金(21007010)

教育部博士点基金项目(20090075120007)

上海科委项目(09230500200)

交通运输部科技项目(2010353343290)

湖南省交通厅科研项目(200908)资助.

Toxicity and mechanism of Cu(Ⅱ),Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli under weak acidic conditions

1.
College of Environmental Science and Engineering, Donghua Unviersity, Shanghai, 201620, China;
2.
Department of Material and Environmental Chemistry, Utsunomiya University, Utsunomiya, 321-0904, Japan
Fund Project:

摘要: 比较了弱酸性条件下Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)单独或加入抗坏血酸(L-AscA)对大肠杆菌(E.coli)的毒性,深入分析了Cu(Ⅱ)/L-AscA体系的特性;通过电子自旋共振(ESR)定量分析羟基自由基(·OH)浓度以分析其毒性机理.结果表明,pH 4.0下L-AscA促进了Cu(Ⅱ)、Fe(Ⅲ)而非Cr(Ⅲ)的毒性,三者毒性Cu(Ⅱ)>Cr(Ⅲ)>Fe(Ⅲ).通常被认为无毒的Cr(Ⅲ)却在0.2 mmol·L-1, pH 4.0时表现出了很高的杀菌率.与0.01% L-AscA共存时,Cu(Ⅱ)为200、20 μmol·L-1和2、0.2 μmol·L-1下,E.coli的存活率分别在30 min和2 h内迅速降至零,且该体系对自然水体中分离所得的其它7种菌株同样具有明显的制御作用.ESR结果表明L-AscA的加入使200 μmol·L-1 Cu(Ⅱ)反应体系的·OH浓度约提高两倍,·OH浓度呈Cu(Ⅱ)浓度依赖.但在Fe(Ⅲ)、Cr(Ⅲ)/L-AscA体系中未检测到·OH,表明三者对细胞的致毒机制存在明显差异.
- 过渡金属 /
- 抗坏血酸 /
- 毒性 /
- ESR
Abstract: Toxicity of three transition metal ions Cu(Ⅱ), Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli was investigated under weakly acidic conditions, both in the presence and absence of ascorbic acid (L-AscA). Furthermore, their cell killing mechanisms which showed great difference, were studied by electron spin resonance (ESR). L-AscA enhanced Cu(Ⅱ) and Fe(Ⅲ) but notCr(Ⅲ) bactericidal efficiency at pH 4.0, under which all cell death was shown in 30 minutes after full contact with 200 μmol·L-1, 20 μmol·L-1 Cu(Ⅱ) based on viable cell counting, and 2 hours with 2 μmol·L-1, 0.2 μmol·L-1 Cu(Ⅱ). Cr(Ⅲ) alone, which is widely recognized as innocuous and stable, at 0.2 mmol·L-1, pH 4.0, also showed surprisingly high disinfection capability. Toxicity in the presence of L-AscA was as follows: Cu(Ⅱ)>Cr(Ⅲ)>Fe(Ⅲ). Furthermore, their cell killing mechanisms turned out to be different. Hydroxyl radical produced by Fenton-like reaction was involved in Cupric toxicity, which has been proved by ESR using the spin trapping 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO). L-AscA almost doubled ·OH concentration produced by 200 μmol·L-1 Cu(Ⅱ), and ·OH concentration was positively related to Cu(Ⅱ). In the case of Fe(Ⅲ) and Cr(Ⅲ), no·OH was detected by ESR. Their toxic mechanism is complicated and remains to be elucidated.
- transition metal /
- L-Ascorbic acid /
- toxicity /
- ESR

[1]	Valko M, Morris H, Cronin M T. Metals, toxicity and oxidative stress[J]. Curr Med Chem, 2005, 12(10): 1161-1208
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徐芳芳, 黄满红, 陈亮, 柿井一男. Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制[J]. 环境化学, 2012, 31(12): 1840-1848.

引用本文:

徐芳芳, 黄满红, 陈亮, 柿井一男. Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制[J]. 环境化学, 2012, 31(12): 1840-1848.

Citation:

Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制

1. 东华大学环境科学与工程学院, 上海, 201620;
2. 日本宇都宫大学材料与环境化学系, 宇都宫, 321-0904

收稿日期: 2012-02-29

基金项目:

国家自然科学基金(21007010)

教育部博士点基金项目(20090075120007)

上海科委项目(09230500200)

交通运输部科技项目(2010353343290)

湖南省交通厅科研项目(200908)资助.

关键词:

过渡金属 /
抗坏血酸 /
毒性 /
ESR

摘要: 比较了弱酸性条件下Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)单独或加入抗坏血酸(L-AscA)对大肠杆菌(E.coli)的毒性,深入分析了Cu(Ⅱ)/L-AscA体系的特性;通过电子自旋共振(ESR)定量分析羟基自由基(·OH)浓度以分析其毒性机理.结果表明,pH 4.0下L-AscA促进了Cu(Ⅱ)、Fe(Ⅲ)而非Cr(Ⅲ)的毒性,三者毒性Cu(Ⅱ)>Cr(Ⅲ)>Fe(Ⅲ).通常被认为无毒的Cr(Ⅲ)却在0.2 mmol·L-1, pH 4.0时表现出了很高的杀菌率.与0.01% L-AscA共存时,Cu(Ⅱ)为200、20 μmol·L-1和2、0.2 μmol·L-1下,E.coli的存活率分别在30 min和2 h内迅速降至零,且该体系对自然水体中分离所得的其它7种菌株同样具有明显的制御作用.ESR结果表明L-AscA的加入使200 μmol·L-1 Cu(Ⅱ)反应体系的·OH浓度约提高两倍,·OH浓度呈Cu(Ⅱ)浓度依赖.但在Fe(Ⅲ)、Cr(Ⅲ)/L-AscA体系中未检测到·OH,表明三者对细胞的致毒机制存在明显差异.

English Abstract

Toxicity and mechanism of Cu(Ⅱ),Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli under weak acidic conditions

1. College of Environmental Science and Engineering, Donghua Unviersity, Shanghai, 201620, China;
2. Department of Material and Environmental Chemistry, Utsunomiya University, Utsunomiya, 321-0904, Japan

Received Date: 2012-02-29

Fund Project:

Keywords:

Abstract: Toxicity of three transition metal ions Cu(Ⅱ), Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli was investigated under weakly acidic conditions, both in the presence and absence of ascorbic acid (L-AscA). Furthermore, their cell killing mechanisms which showed great difference, were studied by electron spin resonance (ESR). L-AscA enhanced Cu(Ⅱ) and Fe(Ⅲ) but notCr(Ⅲ) bactericidal efficiency at pH 4.0, under which all cell death was shown in 30 minutes after full contact with 200 μmol·L-1, 20 μmol·L-1 Cu(Ⅱ) based on viable cell counting, and 2 hours with 2 μmol·L-1, 0.2 μmol·L-1 Cu(Ⅱ). Cr(Ⅲ) alone, which is widely recognized as innocuous and stable, at 0.2 mmol·L-1, pH 4.0, also showed surprisingly high disinfection capability. Toxicity in the presence of L-AscA was as follows: Cu(Ⅱ)>Cr(Ⅲ)>Fe(Ⅲ). Furthermore, their cell killing mechanisms turned out to be different. Hydroxyl radical produced by Fenton-like reaction was involved in Cupric toxicity, which has been proved by ESR using the spin trapping 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO). L-AscA almost doubled ·OH concentration produced by 200 μmol·L-1 Cu(Ⅱ), and ·OH concentration was positively related to Cu(Ⅱ). In the case of Fe(Ⅲ) and Cr(Ⅲ), no·OH was detected by ESR. Their toxic mechanism is complicated and remains to be elucidated.

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Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制

Toxicity and mechanism of Cu(Ⅱ),Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli under weak acidic conditions

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Cu(Ⅱ)、Fe(Ⅲ)和Cr(Ⅲ)在弱酸性条件下对大肠杆菌的毒性和致毒机制

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Toxicity and mechanism of Cu(Ⅱ),Fe(Ⅲ) and Cr(Ⅲ) against Escherichia coli under weak acidic conditions

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