高级搜索

高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

,  ,  ,  , 

downloadPDF

上一篇

下一篇

徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
引用本文: 徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu
XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
Citation: XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu

高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

  • 1.

    浙江省气象科学研究所, 杭州, 310008;

  • 2.

    宁波诺丁汉大学化学与环境工程系, 宁波, 315100

  • 基金项目:

    国家重点研发计划（2016YFC0201900），国家自然科学基金（41303091，91544226），浙江省公益技术研究社会发展项目（2015C33011）和宁波市室内空气污染净化技术创新团队（2017C510001）资助.

Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry

  • 1.

    Zhejiang Institute of Meteorological Sciences, Hangzhou, 310008, China;

  • 2.

    Department of Chemical and Environmental Engineering, The University of Nottingham Ningbo China, Ningbo, 315100, China

  • Fund Project: Supported by the National Key R&D Program of China(2016YFC0201900), the National Natural Science Foundation of China (41303091,91544226), Nonprofit Technology Applied Research Project of the Zhejiang Province(2015C33011) and Ningbo Innovation Team on Indoor Air Pollution Control Technology (2017C510001).

  • 摘要: 为实现真菌气溶胶示踪物的准确、快速、高灵敏测定，通过高效液相色谱和三重四极杆质谱联用，优化相关分析参数，建立了对阿糖醇和甘露醇这2种目标有机示踪物无需衍生化处理即可进行直接检测的快速分析方法.结果显示，在流动相氨水的浓度0.0001%、流速选择0.4 mL·min-1、柱温箱温度45 ℃、负离子模式条件下，2种目标有机示踪物的线性范围是0.01—1 mg·L-1（R2>0.99）， 检测限分别达到5.6 μg·L-1（阿糖醇）、9.4 μg·L-1 （甘露醇）.该方法检测限低、灵敏度高、样品回收率高、重现性好，并具有较好的实用性.
    Abstract: An accurate, fast and sensitive analytical method for fungi aerosol tracers including both arabitol and mannitol has been developed using high performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) without any derivatization process, which is able to greatly reduce sample preparation time. The optimum analytical condition has been established as below:ammonium hydroxide (ω:0.0001%) as mobile phase with a flow rate of 0.4 mL·min-1, oven temperature of 45℃, and negative ion electrospray ionization. Under the abovementioned analytical condition, the linear range of the two organic tracers is 0.01-1 mg·L-1(R2>0.99) and limits of detection (LOD) are 5.6 μg·L-1(arabitol) and 9.4 μg·L-1 (mannitol), respectively. After validation and application to the real aerosol samples, this new method has been proved to be a reliable and cost-effective alternative technique for analysis of both arabitol and mannitol.
  • 加载中
  • [1] BURROWS S M, HOOSE C, POESCHL U, et al. Ice nuclei in marine air:Biogenic particles or dust?[J]. Atmospheric Chemistry and Physics, 2013, 13(1):245-267.
    [2] CORLETT R T. Climate change in the tropics:The end of the world as we know it?[J]. Biological Conservation, 2012, 151(1):22-25.
    [3] SHARMA S, CHAN E, ISHIZAWA M, et al. Influence of transport and ocean ice extent on biogenic aerosol sulfur in the Arctic atmosphere[J]. Journal of Geophysical Research-Atmospheres, 2012, 117(D12):48-50.
    [4] 高敏, 仇天雷, 贾瑞志,等. 北京雾霾天气生物气溶胶浓度和粒径特征[J]. 环境科学, 2014,35(12):4415-4421.

    GAO M, QIU T L, JIA R Z, et al. Concentration and size distribution of bioaerosols at no-haze and haze days in Beijing[J]. Environmental Science, 2014,35(12):4415-4421(in Chinese).

    [5] 胡凌飞,张柯,王洪宝, 等. 北京雾霾天大气颗粒物中微生物气溶胶的浓度及粒谱特征[J]. 环境科学,2015, 36(9):3144-3149.

    HU L F, ZHANG K, WANG H B, et al. Concentration and particle size distribution of microbiological aerosol during haze days in Beijing[J]. Environmental Science, 2015, 36(9):3144-3149(in Chinese).

    [6] 杜睿,周宇光. 北京及周边地区大气近地面层真菌气溶胶的变化特征[J]. 中国环境科学,2010, 30(3):296-301.

    DU R, ZHOU Y G. Variation characteristics of fungi aerosols in the near-surface atmospheric layer in Beijing and surrounding area[J]. China Environmental Science, 2010, 30(3):296-301(in Chinese).

    [7] YANG Y, CHAN C Y, TAO J, et al. Observation of elevated fungal tracers due to biomass burning in the Sichuan Basin at Chengdu City, China[J]. Science of the Total Environment, 2012, 431:68-77.
    [8] ZHANG T, ENGLING G, CHAN C Y, et al. Contribution of fungal spores to particulate matter in a tropical rainforest[J]. Environmental Research Letters, 2010, 5(2):024010.
    [9] FU P, KAWAMURA K, KOBAYASHI M, et al. Seasonal variations of sugars in atmospheric particulate matter from Gosan, Jeju Island:Significant contributions of airborne pollen and Asian dust in spring[J]. Atmospheric Environment, 2012, 55:234-239.
    [10] DESPRES V R, HUFFMAN J A, BURROWS S M, et al. Primary biological aerosol particles in the atmosphere:A review[J]. Tellus Series B-Chemical and Physical Meteorology, 2012, 64:1-58.
    [11] BAUER H, CLAEYS M, VERMEYLEN R, et al. Arabitol and mannitol as tracers for the quantification of airborne fungal spores[J]. Atmospheric Environment, 2008, 42(3):588-593.
    [12] MEDEIROS P M, SIMONEIT B R T. Analysis of sugars in environmental samples by gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2007, 1141(2):271-278.
    [13] SCHUMMER C, DELHOMME O, APPENZELLER B M R, et al. Comparison of MTBSTFA and BSTFA in derivatization reactions of polar compounds prior to GC/MS analysis[J]. Talanta, 2009, 77(4):1473-1482.
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  1071
  • HTML全文浏览数:  1009
  • PDF下载数:  221
  • 施引文献:  0
出版历程
  • 收稿日期:  2017-04-20
  • 刊出日期:  2017-12-15
徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
引用本文: 徐宏辉, 徐婧莎, 何俊, 姜瑜君, 浦静姣. 高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物[J]. 环境化学, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu
XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
Citation: XU Honghui, XU Jingsha, HE Jun, JIANG Yujun, PU Jingjiao. Rapid determination of fungal aerosol tracers using high performance liquid chromatography-tandem mass spectrometry[J]. Environmental Chemistry, 2017, 36(12): 2683-2689. doi: 10.7524/j.issn.0254-6108.2017042001
shu

高效液相色谱-三重四极杆质谱法快速测定真菌气溶胶示踪物

  • 1.  浙江省气象科学研究所, 杭州, 310008;
  • 2.  宁波诺丁汉大学化学与环境工程系, 宁波, 315100
  • 收稿日期:  2017-04-20
基金项目:

国家重点研发计划（2016YFC0201900），国家自然科学基金（41303091，91544226），浙江省公益技术研究社会发展项目（2015C33011）和宁波市室内空气污染净化技术创新团队（2017C510001）资助.

摘要: 为实现真菌气溶胶示踪物的准确、快速、高灵敏测定，通过高效液相色谱和三重四极杆质谱联用，优化相关分析参数，建立了对阿糖醇和甘露醇这2种目标有机示踪物无需衍生化处理即可进行直接检测的快速分析方法.结果显示，在流动相氨水的浓度0.0001%、流速选择0.4 mL·min-1、柱温箱温度45 ℃、负离子模式条件下，2种目标有机示踪物的线性范围是0.01—1 mg·L-1（R2>0.99）， 检测限分别达到5.6 μg·L-1（阿糖醇）、9.4 μg·L-1 （甘露醇）.该方法检测限低、灵敏度高、样品回收率高、重现性好，并具有较好的实用性.

English Abstract

    全文HTML

参考文献 (13)

返回顶部

目录

/

返回文章
返回

Copyright © 2019 中国科学院生态环境研究中心