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溶藻细菌Microbacterium oleivoran的溶藻进程与叶绿素降解动力学

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郭惠娟, 张伟, 张小梅, 毛林强, 张文艺. 溶藻细菌Microbacterium oleivoran的溶藻进程与叶绿素降解动力学[J]. 环境化学, 2019, 38(6): 1274-1281. doi: 10.7524/j.issn.0254-6108.2018082801
引用本文: 郭惠娟, 张伟, 张小梅, 毛林强, 张文艺. 溶藻细菌Microbacterium oleivoran的溶藻进程与叶绿素降解动力学[J]. 环境化学, 2019, 38(6): 1274-1281. doi: 10.7524/j.issn.0254-6108.2018082801
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GUO Huijuan, ZHANG Wei, ZHANG Xiaomei, MAO Linqiang, ZHANG Wenyi. Process of algae-lysis and chlorophyll degradation kinetics of Microbacterium oleivoran bacteria[J]. Environmental Chemistry, 2019, 38(6): 1274-1281. doi: 10.7524/j.issn.0254-6108.2018082801
Citation: GUO Huijuan, ZHANG Wei, ZHANG Xiaomei, MAO Linqiang, ZHANG Wenyi. Process of algae-lysis and chlorophyll degradation kinetics of Microbacterium oleivoran bacteria[J]. Environmental Chemistry, 2019, 38(6): 1274-1281. doi: 10.7524/j.issn.0254-6108.2018082801
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溶藻细菌Microbacterium oleivoran的溶藻进程与叶绿素降解动力学

  • 1.

    常州大学 环境与安全工程学院, 常州, 213164;

  • 2.

    常州市新北环境监测站, 常州, 213000

  • 基金项目:

    国家自然科学基金（41571471）和水体污染控制与治理科技重大专项（2017ZX07202-003/004）资助.

Process of algae-lysis and chlorophyll degradation kinetics of Microbacterium oleivoran bacteria

  • 1.

    School of Environmental & Safety Engineering, Changzhou University, Changzhou, 213164, China;

  • 2.

    Changzhou Xinbei environmental monitoring station, Changzhou, 213000, China

  • Fund Project: Supported by the National Natural Science Foundation of China (41571471)and National Basic Research Program of China(2017ZX07202-003/004)

  • 摘要: 从太湖土著花鲴鱼肝、肠等内脏中筛选出7株具有溶藻功效的菌株，其中溶藻率最高的1株菌命名为GHJ，经DNA测序并构建系统发育树，确定该菌属于微杆菌属（Microbacterium oleivoran），以太湖流域常见藻类——铜绿微囊藻为溶藻对象，以叶绿素a（Chla）含量变化表征溶藻特性，初步揭示溶藻进程中的GHJ菌生长动力学和铜绿微囊藻降解动力学机制，探讨了二者相关关系.结果表明，GHJ的溶藻进程是通过直接溶藻与间接溶藻协同作用，破碎并溶解藻细胞，其在牛肉膏蛋白胨培养基中生长曲线符合Logistic生长模型，动力学方程为Nt=1.31/1+e2.81-0.05t，R2=0.9797.菌藻比为1：12时的溶藻率最高达到99.60%，此条件下叶绿素与溶藻时间之间的关系符合一级动力学模型[Chla]=111.96×e-0.21t，R2=0.8997；所取菌藻体积比在1：8-1：50范围内的叶绿素减少量与溶藻过程时间关系均符合一级动力模型，R2介于0.6897-0.8997之间，GHJ菌在整个溶藻过程中溶藻趋势相同.本研究可为溶藻菌菌种来源和溶藻过程其他工程应用提供基础理论支撑.
    Abstract: Seven strains possessing the ability of algae lysis were screened from the liver and intestine of the indigenous Hemibarbus maculatus, in Taihu Lake. One of them with the highest algae lysis was named as GHJ, and was identified as Microbacterium oleivoran by DNA sequencing and building phylogenetic trees. In this study, Microcystis aeruginosa, which is the usual algae in Taihu Lake Basin, was employed as the target degradation algae. The GHJ algae lysis characteristic was determined with the change in the concentration of chlorophyll. The growth kinetics of GHJ and the kinetic mechanism of degradation of Microcystis aeruginosa in the algae lysis process were preliminarily revealed. In addition, the correlation between the growth kinetics of GHJ and the kinetic mechanism of degration of Microbacterium oleivoran was expored. The results showed that the algae lysis process of GHJ was accomplished by destroying and dissolving algae cells via the synergistic process of direct dissolution and indirect dissolution. The growth curve of GHJ bacteria in beef paste protein medium agreed with the Logistic growth model, and the kinetic equation was Nt=1.31/(1+e(2.81-0.05t)) (R2=0.9797). The algae-lysis ratio is up to 99.60% when the ratio of bacteria to algae is 1:12, and the relationship between chlorophyll and algal lysis time agreed with the first-order kinetic model of[Chla]=111.96×e(-0.21t) (R2=0.9167). When the ratio of bacteria to algae is in the range of 1:8 to 1:50, the relationship between the amount of chlorophyll reduction and the time of algae lysis is agreed with the first-order kinetic model, R2 is between 0.6897 and 0.8997, and GHJ bacteria have the same tendency to dissolve algae across the algae lysis process. This study provides basic theoretical support for the screening source of algae-lysis bacteria and other engineering applications of algal lysis processes.
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  • 收稿日期:  2018-08-28
  • 刊出日期:  2019-06-15

溶藻细菌Microbacterium oleivoran的溶藻进程与叶绿素降解动力学

  • 1.  常州大学 环境与安全工程学院, 常州, 213164;
  • 2.  常州市新北环境监测站, 常州, 213000
  • 收稿日期:  2018-08-28
基金项目:

国家自然科学基金（41571471）和水体污染控制与治理科技重大专项（2017ZX07202-003/004）资助.

摘要: 从太湖土著花鲴鱼肝、肠等内脏中筛选出7株具有溶藻功效的菌株，其中溶藻率最高的1株菌命名为GHJ，经DNA测序并构建系统发育树，确定该菌属于微杆菌属（Microbacterium oleivoran），以太湖流域常见藻类——铜绿微囊藻为溶藻对象，以叶绿素a（Chla）含量变化表征溶藻特性，初步揭示溶藻进程中的GHJ菌生长动力学和铜绿微囊藻降解动力学机制，探讨了二者相关关系.结果表明，GHJ的溶藻进程是通过直接溶藻与间接溶藻协同作用，破碎并溶解藻细胞，其在牛肉膏蛋白胨培养基中生长曲线符合Logistic生长模型，动力学方程为Nt=1.31/1+e2.81-0.05t，R2=0.9797.菌藻比为1：12时的溶藻率最高达到99.60%，此条件下叶绿素与溶藻时间之间的关系符合一级动力学模型[Chla]=111.96×e-0.21t，R2=0.8997；所取菌藻体积比在1：8-1：50范围内的叶绿素减少量与溶藻过程时间关系均符合一级动力模型，R2介于0.6897-0.8997之间，GHJ菌在整个溶藻过程中溶藻趋势相同.本研究可为溶藻菌菌种来源和溶藻过程其他工程应用提供基础理论支撑.

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