NaF对美国白蛾生长发育及生殖的影响
Effects of NaF on Growth, Development and Reproduction of Hyphantria cunea
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摘要: 氟化物是环境污染物之一，严重危害着动植物的健康。本研究采用饲料混药法，测定不同浓度氟化钠(NaF)对美国白蛾6龄幼虫的毒力。NaF处理后10 d的LC50为181.70 mg·L-1(95%置信区间为159.60～209.10 mg·L-1)，LC30为155.60 mg·L-1，最大无观察效应浓度(no observed effect concentration, NOEC)为80.00 mg·L-1。采用亚致死浓度NaF(LC10)处理美国白蛾6龄幼虫，统计幼虫体质量、发育历期、蛹质量、蛹历期、化蛹率、羽化率等生长发育指标和产卵量、受精率、孵化率等生殖能力指标。结果表明，随NaF浓度升高和时间的延长，死亡率呈现上升趋势；亚致死浓度NaF(LC10)处理后，美国白蛾6龄幼虫体型缩小，发育历期延长；化蛹率、蛹质量显著降低和蛹期显著延长，依次为对照组的86.7%、83.3%和115.4%；产卵量、受精率和孵化率显著低于对照组，且NaF对雄性的影响比对雌性更为显著。本研究揭示了NaF对美国白蛾的生长发育和生殖能力影响，为进一步探讨环境污染物氟化物对植食性昆虫的潜在毒性提供了科学依据。Abstract: Fluoride is one of the environmental pollutants, which seriously damage the health of animals and plants. This research adopts diet mixture method to determine the toxicity of different concentrations of sodium fluoride (NaF) to the 6th instar H. cunea larvae. After 10 days of treatment, the median lethal concentration (LC50) and sublethal concentration (LC30) were 181.70 mg·L-1 (the 95% confidence interval was 159.60～209.10 mg·L-1) and 155.60 mg·L-1, respectively, and the maximal non-observed effective concentration (NOEC) was 80.00 mg·L-1. The indices of growth and development, including larval weight, development duration, pupal weight, pupal duration, pupation rate and emergence rate, and the indices of reproductive capacity, including fecundity, fertilization rate and hatching rate, were analyzed using the sublethal concentration (LC10) of NaF to treat the 6th instar H. cunea larvae. The results showed that the mortality rate increased with the increase of NaF concentration and extension of exposure time. After treatment with sublethal concentration (LC10) of NaF, the size of the 6th instar H. cunea larvae was shrinked and the developmental duration was prolonged. The pupation rate and pupal weight were significantly decreased while the pupal duration was significantly prolonged with 86.7%, 83.3% and 115.4% of the control group, respectively. The fecundity, fertilization rate and hatching rate were significantly lower than those of the control group, and the effect of NaF on males was more significant than that of females. This study reveals the effects of NaF on the growth and reproduction of H. cunea, and provides a scientific basis for further exploring the potential toxicity of environmental fluoride pollutants to phytophagous insects.
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Key words:
- NaF /
- Hyphantria cunea /
- reproductive toxicity /
- growth and development
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