斑马鱼中高通量毒性测试方法研究进展和应用现状
Research Progress and Application of Middle/High-throughput Toxicity Testing Methods Using Zebrafish
-
摘要: 基于哺乳动物的传统毒性测试方法存在周期长、成本高、通量低、有动物伦理限制等诸多问题。斑马鱼因为个体小、发育快、易于饲养等特点，适用于构建基于多孔板的中高通量毒性测试方法。本文对基于多孔板的斑马鱼急性毒性、发育毒性、行为和微行为分析等几个中高通量测试方法进行了综述，分析了各自的优缺点及应用现状。另外介绍了高通量测试在自动化操作方面的最近进展。最后分析了斑马鱼中高通量毒性测试方法未来的发展趋势和应用前景，提出应着重开展对现有方法的验证和优化工作，减少对人工的依赖，实现自动化操作和标准化分析，同时开发可更敏感检出发育毒性的分子或者信号通路的测试指标。随着斑马鱼高通量毒性测试方法不断的发展和优化，其在化学物质毒性筛查和风险评估中将发挥越来越重要的作用。Abstract: Traditional mammalian-based toxicity testing methods have many drawbacks such as long test duration, high cost, low throughput and animal ethical restrictions. Zebrafish is suitable for the construction of high-throughput toxicity testing methods due to its outstanding features, such as being smaller in size, rapid growth and easy to feed. In this paper, existing middle and high throughput testing methods for analyzing the acute toxicity, development toxicity and behaviors of zebrafish based on porous plates were reviewed, and their advantages, disadvantages and application status were analyzed. Recent research progress on automatic operations for high-throughput testing was also introduced. The development trend and application prospects of middle/high-throughput toxicity testing methods using zebrafish were discussed as well. It is believed that the validation and refinement of existing methods should be emphasized to improve automation and standardization, Meanwhile, more refined molecular or signaling pathway endpoints for sensitive detection of developmental toxicity should be developed. It is foreseeable that zebrafish-based middle/high-throughput testing methods will play significant roles in toxicity screening and risk assessment of chemicals.
-
Key words:
- high-throughput /
- zebrafish /
- development toxicity /
- toxicity testing
-
-
Attene-Ramos M S, Miller N, Huang R L, et al. The Tox21 robotic platform for the assessment of environmental chemicals:From vision to reality [J]. Drug Discovery Today, 2013, 18(15-16):716-723 Richard A M, Huang R L, Waidyanatha S, et al. The Tox2110K compound library:Collaborative chemistry advancing toxicology [J]. Chemical Research in Toxicology, 2021, 34(2):189-216 Jeong J, Kim D, Choi J. Application of ToxCast/Tox21 data for toxicity mechanism-based evaluation and prioritization of environmental chemicals:Perspective and limitations [J]. Toxicology in Vitro, 2022, 84:105451 Bambino K, Chu J. Zebrafish in toxicology and environmental health [J]. Current Topics in Developmental Biology, 2017, 124:331-367 Horzmann K A, Freeman J L. Making waves:New developments in toxicology with the zebrafish [J]. Toxicological Science, 2018, 163(1):5-12 Howe K, Clark M D, Torroja C F, et al. The zebrafish reference genome sequence and its relationship to the human genome [J]. Nature, 2013, 496(7446):498-503 Mao Y S, Hong K, Liao W, et al. Generation of a novel transgenic zebrafish for studying adipocyte development and metabolic control [J]. International Journal of Molecular Sciences, 2021, 22(8):3994 Yang Z, Chen S H, Xue S L, et al. Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model [J]. Biotechnology Letters, 2018, 40(11):1507-1508 Udvadia A J, Linney E. Windows into development:Historic, current, and future perspectives on transgenic zebrafish [J]. Developmental Biology, 2003, 256(1):1-17 Lai K P, Gong Z Y, Tse W K F. Zebrafish as the toxicant screening model:Transgenic and omics approaches [J]. Aquatic Toxicology, 2021, 234:105813 Organization for Economic Cooperation and Development (OECD). OECD guidelines for the testing of chemicals, section 2:Effects on biotic systems test No. 203:Acute toxicity for fish [R]. Paris:OECD, 2019 Strähle U, Scholz S, Geisler R, et al. Zebrafish embryos as an alternative to animal experiments:A commentary on the definition of the onset of protected life stages in animal welfare regulations [J]. Reproductive Toxicology, 2012, 33(2):128-132 Belanger S E, Rawlings J M, Carr G J. Use of fish embryo toxicity tests for the prediction of acute fish toxicity to chemicals [J]. Environmental Toxicology and Chemistry, 2013, 32(8):1768-1783 Braunbeck T, Kais B, Lammer E, et al. The fish embryo test (FET):Origin, applications, and future [J]. Environmental Science and Pollution Research, 2015, 22(21):16247-16261 Lammer E, Carr G J, Wendler K, et al. Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test? [J]. Comparative Biochemistry and Physiology Toxicology & Pharmacology, 2009, 149(2):196-209 Sobanska M, Scholz S, Nyman A M, et al. Applicability of the fish embryo acute toxicity (FET) test (OECD 236) in the regulatory context of Registration, Evaluation, Authorisation, and Restriction of Chemicals (REACH) [J]. Environmental Toxicology and Chemistry, 2018, 37(3):657-670 Kämmer N, Erdinger L, Braunbeck T. The onset of active gill respiration in post-embryonic zebrafish (Danio rerio) larvae triggers an increased sensitivity to neurotoxic compounds [J]. Aquatic Toxicology, 2022, 249:106240 Hu G, Siu S O, Li S, et al. Metabolism of calycosin, an isoflavone from Astragali radix, in zebrafish larvae [J]. Xenobiotica, 2012, 42(3):294-303 Wang Y H, Lv L, Yu Y J, et al. Single and joint toxic effects of five selected pesticides on the early life stages of zebrafish (Danio rerio) [J]. Chemosphere, 2017, 170:61-67 Xiong Y M, Chen X Y, Li F, et al. Zebrafish larvae acute toxicity test:A promising alternative to the fish acute toxicity test [J]. Aquatic Toxicology, 2022, 246:106143 Zhang G Z, Roell K R, Truong L, et al. A data-driven weighting scheme for multivariate phenotypic endpoints recapitulates zebrafish developmental cascades [J]. Toxicology and Applied Pharmacology, 2017, 314:109-117 Hamm J T, Ceger P, Allen D, et al. Characterizing sources of variability in zebrafish embryo screening protocols [J]. ALTEX, 2019, 36(1):103-120 Malev O, Babič S, Sima Cota A, et al. Combining short-term bioassays using fish and crustacean model organisms with ToxCast in vitro data and broad-spectrum chemical analysis for environmental risk assessment of the river water (Sava, Croatia) [J]. Environmental Pollution, 2022, 292(Pt B):118440 Mikut R, Dickmeis T, Driever W, et al. Automated processing of zebrafish imaging data:A survey [J]. Zebrafish, 2013, 10(3):401-421 Chen C Y, Gu Y Y, Philippe J, et al. Acoustofluidic rotational tweezing enables high-speed contactless morphological phenotyping of zebrafish larvae [J]. Nature Communications, 2021, 12(1):1118 Dong G Q, Wang N, Xu T, et al. Deep learning-enabled morphometric analysis for toxicity screening using zebrafish larvae [J]. Environmental Science & Technology, 2023, 57(46):18127-18138 Silva Brito R, Canedo A, Farias D, et al. Transgenic zebrafish (Danio rerio) as an emerging model system in ecotoxicology and toxicology:Historical review, recent advances, and trends [J]. The Science of the Total Environment, 2022, 848:157665 Colborn T D. Our Stolen Future [M]. New York:Penguin Books, 1996:20-30 Wang H, Tan J T, Emelyanov A, et al. Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio [J]. Gene, 2005, 356:91-100 Chen H, Hu J Y, Yang J, et al. Generation of a fluorescent transgenic zebrafish for detection of environmental estrogens [J]. Aquatic Toxicology, 2010, 96(1):53-61 Mattingly C J, McLachlan J A, Jr Toscano W A. Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (Danio rerio) [J]. Environmental Health Perspectives, 2001, 109(8):845-849 Cooper R, David A, Lange A K, et al. Health effects and life stage sensitivities in zebrafish exposed to an estrogenic wastewater treatment works effluent [J]. Frontiers in Endocrinology, 2021, 12:666656 Tong S K, Mouriec K, Kuo M W, et al. A cyp19a1b-gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells [J]. Genesis, 2009, 47(2):67-73 Poon K L, Wang X G, Lee S G P, et al. Editor's highlight:Transgenic zebrafish reporter lines as alternative in vivo organ toxicity models [J]. Toxicological Sciences, 2017, 156(1):133-148 Gu J, Wang H Y, Zhou L J, et al. Oxidative stress in bisphenol AF-induced cardiotoxicity in zebrafish and the protective role of N-acetyl N-cysteine [J]. The Science of the Total Environment, 2020, 731:139190 Sun M Q, Ding R Y, Ma Y M, et al. Cardiovascular toxicity assessment of polyethylene nanoplastics on developing zebrafish embryos [J]. Chemosphere, 2021, 282:131124 顾杰, 郭敏, 吉贵祥, 等. 利用转基因斑马鱼探究四溴联苯醚(BDE-47)的神经毒性作用[J]. 环境监控与预警, 2020, 12(5):99-104 Gu J, Guo M, Ji G X, et al. Detecting the neurotoxicity effects of 2,2',4,4'-tetrabromodiphenyl ether using transgenic zebrafish [J]. Environmental Monitoring and Forewarning, 2020, 12(5):99-104(in Chinese)
Wang G L, Rajpurohit S K, Delaspre F, et al. First quantitative high-throughput screen in zebrafish identifies novel pathways for increasing pancreatic β-cell mass [J]. eLife, 2015, 4:e08261 Everson J L, Tseng Y C, Eberhart J K. High-throughput detection of craniofacial defects in fluorescent zebrafish [J]. Birth Defects Research, 2023, 115(3):371-389 Orger M B, de Polavieja G G. Zebrafish behavior:Opportunities and challenges [J]. Annual Review of Neuroscience, 2017, 40:125-147 Norton W H J. Zebrafish Protocols for Neurobehavioral Research [M]. New York:Springer New York, 2012:191-203 Franco-Restrepo J E, Forero D A, Vargas R A. A review of freely available, open-source software for the automated analysis of the behavior of adult zebrafish [J]. Zebrafish, 2019, 16(3):223-232 Hussain A, Audira G, Malhotra N, et al. Multiple screening of pesticides toxicity in zebrafish and Daphnia based on locomotor activity alterations [J]. Biomolecules, 2020, 10(9):1224 Tombari R J, Mundy P C, Morales K M, et al. Developmental neurotoxicity screen of psychedelics and other drugs of abuse in larval zebrafish (Danio rerio) [J]. ACS Chemical Neuroscience, 2023, 14(5):875-884 Che X F, Huang Y, Zhong K Y, et al. Thiophanate-methyl induces notochord toxicity by activating the PI3K-mTOR pathway in zebrafish (Danio rerio) embryos [J]. Environmental Pollution, 2023, 318:120861 Kämmer N, Reimann T, Ovcharova V, et al. A novel automated method for the simultaneous detection of breathing frequency and amplitude in zebrafish (Danio rerio) embryos and larvae [J]. Aquatic Toxicology, 2023, 258:106493 Adatto I, Lawrence C, Thompson M, et al. A new system for the rapid collection of large numbers of developmentally staged zebrafish embryos [J]. PLoS One, 2011, 6(6):e21715 Zhang X P, Lu Z, Gelinas D, et al. Batch transfer of zebrafish embryos into multiwell plates [J]. IEEE Transactions on Automation Science and Engineering, 2011, 8(3):625-632 White D, Eroglu A, Wang G, et al. ARQiv-HTS, a versatile whole-organism screening platform enabling in vivo drug discovery at high-throughput rates [J]. Nature Protocol, 2016, 142:2432-2453 马聪. 基于剂量-效应简化转录组预测化学品对斑马鱼的胚胎发育毒性[D]. 南京:南京大学, 2019:37-47 Ma C. Prediction of chemical-induced embryo toxicity using dose-dependent reduced transcriptomics approach [D]. Nanjing:Nanjing University, 2019:37 -47(in Chinese)
Gou X, Ma C, Ji H M, et al. Prediction of zebrafish embryonic developmental toxicity by integrating omics with adverse outcome pathway [J]. Journal of Hazardous Materials, 2023, 448:130958 -
点击查看大图
计量
- 文章访问数: 1989
- HTML全文浏览数: 1989
- PDF下载数: 124
- 施引文献: 0
下载:
